Method for rapidly detecting nuclear polyhedrosis virus by loop-mediated isothermal amplification (LAMP)
A technology for karyotype polyhedrosis and virus, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and the determination/inspection of microorganisms. performance, improved detection efficiency, and consistent sensitivity
Active Publication Date: 2019-12-17
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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Problems solved by technology
However, the existing routine PCR detection of nuclear polyhedrosis virus is time-consuming, relatively complicated to operate, and the steps of the instruments used are cumbersome and costly, and the practicability is affected.
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[0042] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.
[0043] The experimental methods used in the examples of the present invention are conventional methods unless otherwise specified.
[0044] The materials and reagents used in the examples of the present invention can be obtained from commercial sources unless otherwise specified.
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Abstract
The invention provides a method for rapidly detecting a nuclear polyhedrosis virus by loop-mediated isothermal amplification (LAMP). A larch inchworm nuclear polyhedrosis virus F protein is used as atarget gene to design and synthesize a specific loop-mediated isothermal amplification (LAMP) primer set p7, the sequence of the primer set p7 is shown in SEQ ID NO:37 to 42, by using an isothermal amplification technology for detection and analysis, rapid detection of 10 min can be realized, sensitivity reaches 1 million times or above, specificity is good, the sensitivity of fluorescent LAMP isthe same as that of developing LAMP, and thus the detection efficiency and the sensitivity of detecting the nuclear polyhedrosis virus by LAMP are greatly improved.
Description
technical field [0001] The invention relates to the technical field of virus detection, in particular to a method for rapidly detecting nuclear polyhedrosis virus by LAMP. Background technique [0002] Nuclear polyhedrosis virus is a natural control factor of Lepidoptera insects. The virus spreads rapidly and spreads rapidly among species. It is an important control measure and can reduce forestry economic losses. Establishing a rapid and accurate detection technology for nuclear polyhedrosis virus will help to know early whether there is a virus in the pest, whether a large amount of virus needs to be sprayed, and whether the pest is infected by the virus after the control measures are taken to achieve effective control wait. However, the existing conventional PCR method for detecting nuclear polyhedrosis virus has problems such as long time-consuming, relatively complicated operation, cumbersome instrument steps, and high cost, which affect its practicability. [0003] L...
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IPC IPC(8): C12Q1/70C12Q1/6844C12R1/93
CPCC12Q1/701C12Q1/6844C12Q2531/119C12Q2563/107
Inventor 曲良建陈怡萌王少博王玉珠
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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