Primers, probes and kit for detecting 19-Del mutation of epidermal growth factor receptor (EGFR) gene

A kit and gene technology, applied in the direction of biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of insufficient sensitivity, low sensitivity, and inability to meet clinical needs, so as to ensure sensitivity and specificity, Consistent detection sensitivity and improved amplification efficiency
CN110438206AActive Publication Date: 2019-11-12HANGZHOU D A GENETIC ENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU D A GENETIC ENG
Publication Date
2019-11-12

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Abstract

The invention discloses nucleic acid sequences and a kit for detecting 19-Del mutation of an epidermal growth factor receptor (EGFR) gene and belongs to the technical field of molecular diagnosis. Theprimers, the probes and the kit for detecting 19-Del mutation of the EGFR gene can detect 53 common mutation types (covering 99% of mutation occurrence rate or above) of EGFR-TKIs targeting drugs forclinical NSCLC patients with high sensitivity and high specificity. The invention also discloses the kit for detecting 19-Del mutation of the EGFR gene. The kit can realize quantitative detection ofmutation of 19-Del of the EGFR gene in one hole conveniently, quickly, sensitively, specifically and accurately, can be used as the reference basis of EGFR-TKIs targeting drugs and can be used for dynamically monitoring curative effects of drugs in real time.
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Description

technical field

[0001] The invention belongs to the technical field of molecular diagnosis, and in particular relates to a primer, a probe and a kit for detecting EGFR gene 19-Del mutation. Background technique

[0002] In recent years, targeted drugs such as epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) have become important in advanced non-small cell lung cancer (NSCLC). one of the treatment modalities. NCCN, ESMO, CSCO and other domestic and foreign tumor diagnosis and treatment and clinical practice guidelines recommend that all NSCLC patients with adenocarcinoma components must undergo EGFR gene mutation detection to guide the selection of EGFR-TKI targeted therapy.

[0003] A large number of clinical studies have found that patients with non-small cell lung cancer cells carrying EGFR gene sensitive mutations, use EGFR tyrosine kinase inhibitors (EGFR-TKI) such as Iressa (gefitinib), Tarceva (erlotinib) Niger), compared with standard first-li...

Claims

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