Preparation method and application of electropositive polymer fluorescent carbon quantum dots with gene vector function
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A technology of carbon quantum dots and gene carriers, which is applied in the field of preparation of fluorescent carbon quantum dots, can solve the problems of unstable fluorescence properties and low quantum yields, and achieve the effects of improving quantum yields, wide sources, and high quantum yields
Active Publication Date: 2017-05-17
CHANGCHUN UNIV OF TECH
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[0004] The present invention aims to solve the problems of heavy metal ions in the existing semiconductor fluorescent nanoparticles, low quantum yield and
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specific Embodiment approach 1
[0031] Specific embodiment one: the preparation method of the polymer fluorescent carbon quantum dot of the positive gene carrier function of this embodiment, comprises the following steps:
[0032] 1. Add organic acid and cationic high molecular polymer to deionized water, ultrasonically dissolve at room temperature to form a uniform mixed solution; wherein the mass ratio of organic acid and cationic high molecular polymer is 1: (1 ~ 5), organic The mass ratio of acid and deionized water is 1:(10~20);
[0033] 2. Put the mixed solution obtained in step 1 into the reaction kettle, and after heating and reacting at 180-200°C for 5-12 hours, the color of the mixed solution turns dark brown;
[0034] 3. Add 2 times the volume of deionized water to the dark brown solution obtained in step 2, centrifuge at a high speed to remove the bottom solid, and retain the solution;
[0035] 4. Then add 2 to 3 times the volume of deionized water to the solution obtained in step 3, mix well an...
specific Embodiment approach 2
[0036] Embodiment 2: This embodiment differs from Embodiment 1 in that the organic acid molecule in Step 1 is folic acid, ethylenediaminetetraacetic acid, histidine, phthalic acid or ascorbic acid. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the cationic high molecular polymer described in step one is polyethyleneimine, chitosan, polyvinylpyridine, poly(dimethylamino) Ethyl methacrylate, cationic polyacrylamide, polydiallyldimethylammonium chloride, polyamidine or polyvinylamine. Others are the same as in the first or second embodiment.
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Abstract
The invention discloses a preparation method and application of electropositive polymer fluorescent carbon quantum dots with a gene vector function, and relates to the preparation method and application of the fluorescent carbon quantum dots. The invention aims at solving the problems of heavy metal ions, low yield of quanta and unstable fluorescent property in the existing semiconductor fluorescent nano particles. The preparation method comprises the following steps: 1, adding organic acid and a cationic high molecular polymer, performing ultrasonic dissolution at room temperature to form a mixed solution; 2, charging the mixed solution into a reaction kettle, and heating for reaction until the color of the mixed solution turns into black brown; 3, adding water with the volume being 2 times of that of the black brown solution into the black brown solution, removing solid by centrifuging, and reserving the solution; and 4, adding deionized water to the solution, uniformly mixing and then recentrifuging the solution, removing solid, and then performing dialyses treatment to obtain the water solution of polymer carbon quantum dots. The polymer fluorescent carbon quantum dots synthesized by the method have superior fluorescent stability and high yield of quanta which is over 60%. The preparation method is used for preparing the polymer fluorescent carbon quantum dots.
Description
technical field [0001] The invention relates to a preparation method and application of fluorescent carbon quantum dots. Background technique [0002] Cancer, also known as malignant tumor, is a disease caused by the abnormal mechanism of controlling cell growth and proliferation. Cancer entangles human beings like a ghost, swallowing millions of lives every year, causing people to "talk about cancer." In developed countries such as Europe and the United States, cancer is the second cause of human death, and it also ranks second or third in developing countries. The multiple and harmful nature of cancer has made the research on cancer treatment methods a global hot issue. Recently, the introduction of gene therapy provides a fundamental therapy for the treatment of cancer. Gene therapy refers to the use of molecular biology methods to introduce normal genes or genes with therapeutic effects into cancerous cells or tissues of the human body in a certain way, so as to corre...
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