37results about How to "Realize photothermal therapy" patented technology

The invention relates to a preparation method for polydopamine-coated polyethyleneimine-stablized gold-nanometer-star photothermal treatment agent. The preparation method comprises the following steps: preparing a gold seed solution from chloroauric acid and sodium citrate; activating mercaptoacetic acid with EDC, then adding an aqueous PEI solution and carrying out a reaction, dialysis, cooling and drying so as to obtain PEI-SH; adding dopamine hydrochloride into a Tris buffer so as to obtain dopamine Tris buffer; adding the gold seed solution into a chloroauric acid solution, adding a AgNO3 solution and an ascorbic acid solution under stirring, adding PEI-SH after stirring reaction and carrying out a reaction so as to obtain AuNSs-PEI; and adding AuNSs-PEI into the dopamine Tris buffer and carrying out stirring and centrifugation so as to obtain the photothermal treatment agent. The preparation method has the advantages of simplicity, mild reaction conditions, easy operation and industrialization prospects; and the prepared star-like nanometer agent has application potential in the fields of CT imaging diagnosis and photothermal treatment of cancers.

The invention provides a preparation method of Cu2MoS4 nano-enzyme, and the method comprises the following steps: mixing cuprous oxide, an organic carbon source, an inorganic molybdenum source and anorganic sulfur source in an organic solvent, and carrying out high-temperature calcination to obtain the hollow Cu2MoS4 nano-enzyme. Compared with the prior art, the Cu2MoS4 nano-enzyme prepared by the invention has catalase activity and greatly relieves tumor hypoxia, and meanwhile, the nano-enzyme also has peroxidase activity and can consume glutathione overexpressed in a tumor, so that an antioxidant defense system of the tumor is destroyed, and the chemokinetic treatment efficiency is enhanced; two pairs of redox couples exist, the redox couples can react with hydrogen peroxide to generatehigh-toxicity hydroxyl radicals, a remarkable chemical and dynamic curative effect is achieved, meanwhile, the nano-enzyme has strong absorption in the whole near-infrared area, and photo-thermal therapy can be achieved; and under laser excitation, superoxide anion free radicals can be generated and used for photodynamic therapy.

The invention discloses a green preparation method of sea urchin shaped nanogold and application of the nanogold in tumor imaging and treatment. Chloroauric acid is mainly used as a gold source, a stabilizer, a reducing agent and a growth inhibitor are successively added, and the nanogold is prepared by adopting a one-step synthesis method. The method is simple, mild, green and friendly to the environment. The synthesized sea urchin shaped nanogold can serve as a contrast agent to be applied to multiple medical imaging modes such as opto-acoustic imaging (PA), positron emission computed tomography imaging (PET) and infrared thermal imaging (TI), patients can obtain multiple diagnostic effects only by needing to bear one contrast agent for dose, the dosage of contrast agent can be reduced, and the toxic and side effects are thus reduced. In addition, the material can achieve conversion of light and heat under near-infrared light irradiation of 808 nm, can absorb a large number of X rays, has higher light-heat conversion efficiency, and can be used for the preparation of a therapeutic agent for light and heat treatment (PTT), radiotherapy (RT), or combined therapy of PTT / RT of tumor cells.

The invention relates to a polydopamine-covered magnetic mesoporous silica nano-material as well as a preparation and an application thereof. The polydopamine-covered magnetic mesoporous silica nano-material is prepared by the following steps: ultrasonically dispersing HMSs in a solvent, and adding APTES, so as to obtain HMSs-NH2; then, adding an activated Fe3O4NPs solution, and conducting stirring and centrifuging so as to obtain HMSs-Fe3O4NPs; and then, dispersing the HMSs-Fe3O4NPs in a trimethylaminomethane hydrochloride buffer solution Tris-HCl containing dopamine hydrochloride, implementing open stirring at room temperature, and conducting centrifuging and washing, so that the polydopamine-covered magnetic mesoporous silica nano-material HMSs-Fe3O4 / PD NPs is obtained. The preparationmethod provided by the invention is broad in raw material source, low in cost and easy for operation and separation; the prepared nano-material is uniform in grain size distribution and excellent in water solubility and bio-compatibility, and the nano-material is applied to magnetic resonance imaging and photo-thermal and radiotherapy combined treatment; and a broad application prospect is achieved.

The invention relates to a preparation method of a hollow silicon-star gold core / shell nano material for wrapping adriamycin. The preparation method comprises the following steps of dissolving an HMSs (Hollow Mesoporous Silica Sphere), ultrasonically dispersing the dissolved HMSs, adding MPTMS (Mercaptopropyltrimethoxysilane) into the dispersed HMSs, introducing nitrogen into an obtained first mixture, refluxing the obtained first mixture in an oil bath, so as to obtain HMSs-SH (Hollow Mesoporous Silica Sphere-Sulfhydryl), afterwards, dissolving the HMSs-SH in water, adding an Au NPs (Aurum Nanoparticles) solution into an obtained second mixture, agitating the obtained second mixture, so as to obtain an HMSs / Au seed, dissolving the HMSs / Au seed in water, ultrasonically dispersing an obtained third mixture, adding a chloroauric acid solution into the dispersed third mixture, agitating an obtained fourth mixture, adding a silver nitrate solution and an ascorbic acid solution into the agitated fourth mixture, continuously agitating an obtained fifth mixture, so as to obtain an HMSs / Au NSs (Normal Saline Solution), dispersing the HMSs / Au NSs in water, adding an HS-PEG-RGD (Hassium-Polyethylene Glycol-Arginine-Glycine-Aspartic Acid) aqueous solution into an obtained sixth mixture, agitating an obtained seventh mixture, so as to obtain an HMSs / Au-PEG-RGD NSs, afterwards, dispersing the HMSs / Au-PEG-RGD NSs into ultrapure water, adding a DOX*HCL aqueous solution into an obtained eighth solution, agitating an obtained ninth mixture in a light-shielding manner, so that the hollow silicon-star gold core / shell nano material for wrapping the adriamycin is obtained. The nano material provided by the invention owns a specific targeting effect and excellent biocompatibility on a U87MG cell, has a synergistic enhancement effect on chemical therapy and photothermal therapy, and is wide in application prospect.

The invention relates to a photothermal preparation based on biliverdin, comprising biliverdin, biliverdin gel, biliverdin- Metal chelates, biliverdin-Biomolecular complex, biliverdin-Biomolecule-Metal functional assembly. The photothermal preparation has the following advantages: 1) near-infrared absorption and good tissue penetration depth, The preparation of this series of biliverdin-based photothermal preparations is of great significance for the development of multifunctional phototherapeutic drugs, and has great potential applications in tumor, dermatosis (such as pigment diseases), vascular diseases and so on. 3) Biocompatibility and metabolic mechanism are clear.

The invention relates to a preparation method and application of a drug delivery system of a targeting gold-silver alloy nanocage, in order to effectively solve the problems of an existing tumor treatment medicines which are high in side effect and poor in treatment effect; the system is a drug delivery system formed by loading a gene drug on a targeting gold-silver alloy nanocage gene vector, wherein the mass ratio of the targeting gold-silver alloy nanocage gene vector to the gene drug is at ((1-12)*106) to 1; and the targeting gold-silver alloy nanocage gene vector is formed by linking targeting tumor molecule folic acid and a cationic polymer, namely polyethyleneimine (PEI), to the surface of a gold-silver alloy nanocage by virtue of a chemical bond or an electrostatic interaction. The drug delivery system disclosed by the invention is good in physical and chemical stability, simple and feasible in preparation condition, rich in raw material source, low in cost and low in side effect; and the drug delivery system can be used for effectively inhibiting tumor cell proliferation in coordination with thermal therapy by loading the gene drug and targeting to tumor parts, being an innovation of vector in tumor gene therapy and photo-thermal therapy.

The invention provides application of a covalent organic framework material to preparation of one or a plurality of tumor treatment medicine, photodynamic therapy medicaments and photothermal therapymedicaments. The covalent organic framework material is of a structure shown as a formula (I). The covalent organic framework material of the structure shown as the formula (I) is used for tumor treatment medicine, photodynamic therapy medicaments and photothermal therapy medicaments; after the optical excitation, the heat can be released, so that the photothermal therapy of the tumor is realized;the photodynamic and photothermal synergetic therapy can be realized under the single wavelength, so that the powerful cell killing is realized. In addition, the covalent organic framework material has an ERP effect; the enrichment of nanosheets in an in vivo experiment is improved; meanwhile, the photoacoustic imaging capability is realized; the optimal enrichment time of the covalent organic framework material can be determined, so that the tumor treatment under the imaging guidance is realized. The formula is shown in description.

The invention discloses application of aspirin in preparation of a platelet targeted drug delivery system. According to the invention, aspirin is utilized to inhibit platelet aggregation in vitro, prevent platelet deformation and maintain a relatively high proportion of complete platelets, so that relatively high encapsulation efficiency of nanoparticles and antitumor drugs is further realized, aneffective antitumor effect is realized, and the use process is safe and efficient.

The invention belongs to the technical field of photothermal reagents, and particularly relates to a photothermal reagent and synthesis thereof and application of serving as the photothermal reagent under the excitation of a near-infrared 808-nm laser.The designed compound has a property of pH sensitivity and can identify tumor cells by responding to a cellular microenvironment.In the tumor cells, the compound in a weak acid environment can serve as the photothermal reagent to achieve a photothermal therapy under the induction of near-infrared light.In normal cells, due to the fact that the normal cells are alkalescent, a photothermal reaction almost does not exist under the induction of the near-infrared light.

The present invention relates a PH-sensitive block polymer, a FRET (fluorescence resonance energy transfer) composite and their preparation methods; the PH-sensitive block polymer has a structure of polyhistidine-polyethylene glycol-polycaprolactone-lipoic acid, wherein the polyethylene glycol has a molecular weight of 2000-5000, the polycaprolactone has a molecular weight of 2000-10000, and chain segment length of the polyhistidine is composed of 10-30 histidines. The functionalized block copolymer polyhistidine-polyethylene glycol-polycaprolactone-lipoic acid (PLH-PEG-PCL-LA) is used to construct an integrated diagnostic and treatment model that is based on fluorescence resonance energy transfer (FRET) principle, may perform specific near-infrared imaging for a tumor acid microenvironment and engages in therapy. The model can mark a tumor acidic microenvironment to allow for real-time fluorescence imaging and can also transfer gold rods that are photosensitive particles to provide photothermal therapy for tumors.

The invention discloses a preparation method of a nucleolus targeted photo-thermal reagent based on perylene bisimide and an application thereof. The preparation method comprises the following steps: firstly, reacting liquid bromine with perylene anhydride to obtain bromine-substituted perylene bisimide; and then introducing substituent groups into an imide site and a bay site through a reaction between amino and acid anhydride and a nucleophilic substitution reaction between amino and bromine, so as to obtain the perylene bisimide-based nucleolus targeted photo-thermal reagent. The photo-thermal reagent designed and synthesized by the invention is a compound capable of generating strong specific binding with nucleic acid, the binding force enables the photo-thermal reagent to be rapidly taken by cells (within three minutes) and enriched to a nucleolus part with concentrated DNA and RNA, and the targeting effect on the nucleolus is superior to that of a commercial dye nucleolus green. The photothermal reagent designed and synthesized by the invention is good in water solubility, good in light, heat and chemical stability, high in absorbance in a near-infrared region, good in photothermal conversion efficiency and excellent in photothermal killing effect on tumor cells, and can realize photothermal therapy of cancer cells.

The invention discloses an anti-tumor nano-medicine based on hierarchical release and a preparation method thereof. The preparation method includes coating anti-tumor medicine paclitaxel with nano-liposome so as to obtain paclitaxel nano-liposome; mixing a gold nanoparticle solution with the paclitaxel nano-liposome, adding a gold chloride solution and hydroxylamine hydrochloride, and incubating so as to obtain branched gold-spherical-shell-modified paclitaxel nano-liposome; subjecting dopamine to polymerizing in a Tris-HCl buffer solution so as to obtain a polydopamine solution, and then adding doxorubicin to obtain a doxorubicin-loaded polydopamine solution; mixing the prepared branched gold-spherical-shell-modified paclitaxel nano-liposome with the doxorubicin-loaded polydopamine, incubating and centrifugally purifying so as to obtain the final nano-medicine. The anti-tumor nano-medicine prepared by the preparation method solves the problem of insufficient medicine delivery depth intraditional tumor treatment, combines a local treatment mode with a systemic treatment mode and shows excellent biological safety and tumor inhibiting capability.

The invention provides a tumor diagnosis and treatment medicine. The tumor diagnosis and treatment medicine comprises an indocyanine green compound and albumin, wherein the indocyanine green compoundis combined with albumin through electrostatic interaction; and the structural formula of the indocyanine green compound is shown in the description. The tumor diagnosis and treatment medicine can simultaneously realize fluorescence imaging and photothermal therapy, so that diagnosis and treatment integration is achieved. Besides, the tumor diagnosis and treatment medicine has good targeting property and biocompatibility, has relatively long circulation time in vivo, and can meet the requirements of clinical application. The invention further provides a preparation method of the tumor diagnosis and treatment medicine.

The invention discloses a preparation method and application of hydrogel based on poly-N-PPG-PEG, the preparation method comprises the steps that firstly, a poly-N-PPG-PEG nano-composite is prepared,then PPG-PEG nano-composite powder and cyclodextrin (alpha-CD) are prepared into aqueous solutions with certain concentrations respectively, the aqueous solutions are mixed uniformly and stood at theroom temperature for a period of time, and the poly-N-PPG-PEG is prepared. The ultraviolet absorption peak of the prepared hydrogel is shifted to a near-infrared second window, the laser penetration depth can be increased through laser radiation of the near-infrared second window, the absorption of laser by a living tissue is reduced, and damage to the normal tissue is reduced. The hydrogel is injectable type intelligent hydrogel with temperature and shear sensitivity double response, can load chemotherapy drugs so that the drugs can be released at fixed points as required, and belongs to thephoto-thermal hydrogel carrying system for enhancing chemotherapy and photo-thermal combined treatment.

The invention provides an application of an activable photosensitizer in the preparation of a tumor photothermal therapeutic reagent. The provided activable photosensitizer itself has no photothermaleffects, and can produce obvious photothermal effects when the activable photosensitizer is reduced by the nitroreductase in the tumor hypoxic environment, and thereby the targeted photothermal treatment of the tumor is realized without affecting normal cells.

The invention discloses targeted ZIF-8-polydopamine prodrug nanoparticles and a preparation method and application thereof. The preparation method comprises the steps that dopamine-adriamycin, dopamine and zinc chloride are dissolved in a mixed solvent of ethanol / deionized water, then ammonia water is dropwise added, and the pH value of the solution is adjusted to 8.0-8.5; after a stirring reaction, an ethanol / deionized water mixed solution of 2-methylimidazole is dropwise added, the stirring reaction continues, and after the reaction is finished, the ZIF-8-polydopamine prodrug nanoparticles are obtained through dialysis; and finally, the ZIF-8-polydopamine prodrug nanoparticles and polyethylene glycol-folic acid are mixed and added into deionized water, and after the stirring reaction, the targeted ZIF-8-polydopamine prodrug nanoparticles are obtained through dialysis. The targeted ZIF-8-polydopamine prodrug nanoparticles and the preparation method and application thereof are used for solving the problems that in the prior art, a nano-drug is single in structure and lacks active targeting, and the problems of chemotherapy and photothermal therapy combined treatment and the like.

The invention relates to the technical field of chemical synthesis, in particular to a compound, a preparation method thereof, a fluorescent probe and an antitumor drug. The invention discloses a compound as shown in a formula (I) which is described in the specification. The compound is accurately targeted and recognized through a hyaluronic acid ligand-receptor mediation effect; the overexpressedesterase in tumor cells releases TP-PPh3 by destroying an ester bond between the TP-PPh3 and hyaluronic acid, and fluorescence quenched by aggregation of the TP-PPh3 can be recovered. At mitochondrial respiration period, the proton pump in the membrane transports protons to the space in the mitochondrial membrane to form a mitochondrial transmembrane potential with high negativity, so that triphenylphosphine cations have the mitochondrial targeting capability, a mitochondrial region of a tumor can emit red fluorescence under the irradiation of near-infrared light, and fluorescence imaging ofthe tumor part is realized. When a laser of 650-980nm is used for irradiating tumor tissues, the TP-PPh3 can convert absorbed light energy into heat energy, so that tumors are ablated, and photothermal therapy is realized.

The invention discloses a gold / iron carbide heterogeneous nanometer particle and a preparation method and application thereof. The gold / iron carbide heterogeneous nanometer particle is of a janus heterogeneous structure and can serve as a bio-imaging probe, functional modification is conducted through the bio-imaging probe, and application of a photo-thermal treatment probe under guidance of multimode imaging (nuclear magnetic resonance imaging / multispectral photoacoustic tomography imaging / computerized tomography imaging) is achieved. The probe targets tumor cells in a specificity mode, and selective photo-thermal killing for the specific tumor cells can be achieved; and at the same time, due to optical and magnetic properties of the probe, the probe can serve as a contrast agent of multimode imaging to monitor the tumor therapeutic effect.

The invention discloses a composite nano material used for early diagnosis and treatment of tumors and a preparation method thereof. The novel nanomaterial is a composite of bismuth sulfide nanoparticles and gold nanoclusters. The preparation method is as follows: first, use bovine serum albumin (BSA) as a stabilizer and reducing agent to prepare bismuth sulfide nanoparticles (Bi 2 S 3 NPs), followed by the addition of chloroauric acid, Au 3+ In situ growth into gold nanoclusters (AuNCs) under alkaline conditions, and then bismuth sulfide-gold nanoclusters (Bi 2 S 3 ‑AuNCs) composites. Thanks Bi 2 S 3 With the functions of photothermal therapy and CT imaging, AuNCs has the ability of fluorescence imaging, AuNCs and Bi 2 S 3 NPs have photothermal therapy synergistic enhancement, and thus the formed complex can achieve better fluorescence imaging and CT imaging-guided photothermal therapy.

The present invention provides the application of one or more of covalent organic framework materials in the preparation of drugs for treating tumors, photodynamic therapy and photothermal therapy; the covalent organic framework materials have the formula (I ) structure shown. In the present invention, the covalent organic framework material having the structure of formula (I) is used in drugs for treating tumors, drugs for photodynamic therapy and drugs for photothermal therapy, which can release heat after light excitation, thereby realizing photothermal therapy of tumors , and the synergistic therapy of photodynamic and photothermal can be realized under a single wavelength, thus achieving powerful killing of cells. In addition, the present invention also found that the covalent organic framework material has an EPR effect, which improves the enrichment of nanosheets in vivo experiments. At the same time, it has the ability of photoacoustic imaging, and can determine the optimal enrichment time of the covalent organic framework material, thereby realizing Imaging-guided tumor therapy.

The present invention relates to a photothermal preparation based on biliverdin, including: biliverdin, biliverdin gel, biliverdin-metal chelate, biliverdin-biomolecular complex, biliverdin-biomolecule-metal functional assembly. The prepared photothermal preparation has the following advantages: 1) near-infrared absorption and good tissue penetration depth; 2) high photothermal conversion efficiency; 3) biocompatibility and clear metabolic mechanism. The preparation of this series of biliverdin-based photothermal preparations is of great significance for the development of multifunctional phototherapeutic drugs, and has great application potential in tumors, skin diseases (such as pigmentary diseases), and vascular diseases.

The invention discloses a composite nanosphere as well as a preparation method and application thereof. The composite material nanosphere comprises an inner core particle, a metal nano shell layer, a mercapto acid modification layer and a polyethyleneimine compound modification layer, the inner core particles are formed by coating a fluorocarbon compound with a film-forming substance; the surface of the inner core particle is coated with the metal nano shell layer; the sulfydryl acid modification layer is connected to the metal shell layer through sulfydryl; the polyethyleneimine compound layer is connected to the mercapto acid modified layer through an amido bond. The composite material nanosphere has multiple functions of photothermal therapy, enhancement of macrophage gene transfection, ultrasonic enhancement of imaging and the like.

The invention discloses an NO-loaded supramolecular polypeptide nano-drug as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The NO-loaded supramolecular polypeptide nano-drug is prepared from pyridine-poly (L-cysteine)-S-nitroso group, column [5] arene, adriamycin and indocyanine green. The NO-loaded supramolecular polypeptide nano-drug is prepared through the host-guest interaction of water-soluble column [5] arene and pyridinium salt, and is used for simultaneously conveying a chemotherapeutic drug adriamycin and a photothermal conversion agent indocyanine green. Under irradiation of near-infrared light, indocyanine green absorbs light energy and converts the light energy into heat energy, and meanwhile, the heat energy enables the nano-drug to release NO, so that multidrug resistance of tumors is reversed, the chemotherapy effect of adriamycin is synergistically promoted, and triple synergistic treatment of photo-thermal treatment-NO gas treatment-chemotherapy is formed.

The invention discloses a green preparation method of sea urchin shaped nanogold and application of the nanogold in tumor imaging and treatment. Chloroauric acid is mainly used as a gold source, a stabilizer, a reducing agent and a growth inhibitor are successively added, and the nanogold is prepared by adopting a one-step synthesis method. The method is simple, mild, green and friendly to the environment. The synthesized sea urchin shaped nanogold can serve as a contrast agent to be applied to multiple medical imaging modes such as opto-acoustic imaging (PA), positron emission computed tomography imaging (PET) and infrared thermal imaging (TI), patients can obtain multiple diagnostic effects only by needing to bear one contrast agent for dose, the dosage of contrast agent can be reduced, and the toxic and side effects are thus reduced. In addition, the material can achieve conversion of light and heat under near-infrared light irradiation of 808 nm, can absorb a large number of X rays, has higher light-heat conversion efficiency, and can be used for the preparation of a therapeutic agent for light and heat treatment (PTT), radiotherapy (RT), or combined therapy of PTT / RT of tumor cells.