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Preparation method and application of drug delivery system of targeting gold-silver alloy nanocage

A gold-silver alloy and delivery system technology, which is applied in the field of medicine, can solve the problems of large side effects of drugs and poor treatment effects, and achieve the effects of small side effects, simple and feasible preparation conditions, and rich sources of raw materials

Inactive Publication Date: 2015-12-09
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above situation and overcoming the defects of the prior art, the purpose of the present invention is to provide a preparation method and application of a drug delivery system targeting gold-silver alloy nanocages, which can effectively solve the problem of large side effects and poor therapeutic effect of existing tumor treatment drugs. good question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In concrete implementation, the present invention can be realized by the following steps:

[0022] 1) Put 10mg of folic acid, 8mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and 5mg of N-hydroxysuccinimide in 20ml of PBS buffer with a pH value of 5, at room temperature Stir magnetically for 12 hours to obtain an activated folic acid solution;

[0023] 2) Weigh 60 mg of PEG with active groups and add it to the activated folic acid solution, adjust the pH value to 8 with 0.1M NaOH solution, stir magnetically at room temperature for 18 hours, and dialyze with a dialysis bag with a molecular weight of 3500Da for 72 hours to remove unreacted folic acid. Get SH-PEG-NH with active group PEG 2 reaction solution;

[0024] 3) Add 300ml of gold-silver alloy nanocage to the reaction solution prepared in step 2), stir and mix evenly, adjust the pH of the solution to 9 with 0.1M NaOH solution, magnetically stir at room temperature for 48h, and centrifuge at 1200...

Embodiment 2

[0028] In concrete implementation, the present invention can be realized by the following steps:

[0029] 1) Put 9mg of folic acid, 7.5mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and 4.5mg of N-hydroxysuccinimide in 20ml of PBS buffer with a pH value of 5 , stirred magnetically at room temperature for 12 hours to obtain an activated folic acid solution;

[0030] 2) Weigh 50 mg of PEG with an active group and add it to the activated folic acid solution, adjust the pH value to 7 with 0.1M NaOH solution, stir magnetically at room temperature for 18 hours, and dialyze for 72 hours with a dialysis bag with a molecular weight of 3500Da to remove unreacted folic acid. Get SH-PEG-NH with active group PEG 2 reaction solution;

[0031] 3) Add 250ml of gold-silver alloy nanocages to the reaction solution prepared in step 2), stir and mix evenly, adjust the pH of the solution to 9 with 0.1M NaOH solution, magnetically stir at room temperature for 48 hours, and cen...

Embodiment 3

[0035] In concrete implementation, the present invention can be realized by the following steps:

[0036] 1) Put 8mg of folic acid, 7mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and 4mg of N-hydroxysuccinimide in 20ml of PBS buffer with a pH value of 5, at room temperature Stir magnetically for 12 hours to obtain an activated folic acid solution;

[0037] 2) Weigh 40 mg of PEG with active groups and add it to the activated folic acid solution, adjust the pH value to 9 with 0.1M NaOH solution, stir magnetically at room temperature for 18 hours, and dialyze with a dialysis bag with a molecular weight of 3500Da for 72 hours to remove unreacted folic acid. Get SH-PEG-NH with active group PEG 2 reaction solution;

[0038] 3) Add 200ml of gold-silver alloy nanocages to the reaction solution prepared in step 2), stir and mix evenly, adjust the pH value to 9 with 0.1M NaOH solution, magnetically stir at room temperature for 48h, and centrifuge at 12000r / min for...

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Abstract

The invention relates to a preparation method and application of a drug delivery system of a targeting gold-silver alloy nanocage, in order to effectively solve the problems of an existing tumor treatment medicines which are high in side effect and poor in treatment effect; the system is a drug delivery system formed by loading a gene drug on a targeting gold-silver alloy nanocage gene vector, wherein the mass ratio of the targeting gold-silver alloy nanocage gene vector to the gene drug is at ((1-12)*106) to 1; and the targeting gold-silver alloy nanocage gene vector is formed by linking targeting tumor molecule folic acid and a cationic polymer, namely polyethyleneimine (PEI), to the surface of a gold-silver alloy nanocage by virtue of a chemical bond or an electrostatic interaction. The drug delivery system disclosed by the invention is good in physical and chemical stability, simple and feasible in preparation condition, rich in raw material source, low in cost and low in side effect; and the drug delivery system can be used for effectively inhibiting tumor cell proliferation in coordination with thermal therapy by loading the gene drug and targeting to tumor parts, being an innovation of vector in tumor gene therapy and photo-thermal therapy.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of a drug delivery system (preparation) targeting gold-silver alloy nanocages. Background technique [0002] Gene therapy is the introduction of target genes into specific tissues and cells of patients for proper expression to correct or compensate diseases caused by gene defects or abnormalities, so as to achieve the purpose of treating diseases. Compared with traditional treatment methods, gene therapy has indisputable superiority. It corrects the abnormal genes that cause diseases from the root, can selectively treat a variety of diseases that seriously threaten human health, and has achieved certain therapeutic effects. At present, gene therapy has become a new research hotspot in the medical field. However, exposed gene therapy drugs have disadvantages such as easy degradation by ribozymes, poor endocytosis, and poor cell targeting ability. Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K41/00A61K47/02A61K47/34A61K47/12A61K31/7088A61P35/00
Inventor 胡玉荣黄胜楠邱晓静鲍士金王静闫莉娟李焕杰王卫萍刘莹张宁霞牛振喜
Owner ZHENGZHOU UNIV
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