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A kind of preparation method of polydopamine-encapsulated polyethyleneimine-stabilized gold nanostar photothermal therapeutic agent

A technology of polyethylenimine and polydopamine, which is applied in the preparation of X-ray contrast agents, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of high material concentration and the inability to realize tumor photothermal therapy etc. to achieve the effects of low raw material cost, good X-ray attenuation and absorption performance, and good attenuation ability

Inactive Publication Date: 2018-04-06
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method uses a high concentration of materials in the photothermal treatment of cancer, and cannot achieve photothermal treatment of tumors within the material safety range.
[0004] A search of literature and patents on CT contrast agents and photothermal therapy at home and abroad shows that there is no report on the use of polydopamine-coated gold nanostar structures for CT imaging and photothermal therapy of tumors.

Method used

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  • A kind of preparation method of polydopamine-encapsulated polyethyleneimine-stabilized gold nanostar photothermal therapeutic agent
  • A kind of preparation method of polydopamine-encapsulated polyethyleneimine-stabilized gold nanostar photothermal therapeutic agent
  • A kind of preparation method of polydopamine-encapsulated polyethyleneimine-stabilized gold nanostar photothermal therapeutic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) 500mg sodium citrate (Na 3 C 6 h 5 o 7 2H 2 O, Mw=294.1) was added into 49.5mL water to prepare 1% sodium citrate solution. Then heat 10mL aqueous solution of 1mM chloroauric acid to boiling, add 1.5mL 1% sodium citrate solution therein, stir and react under boiling conditions for 10 minutes, cool to room temperature and filter with 0.22μm nitrocellulose membrane to obtain The golden seed solution, stand for subsequent use;

[0048] (2) Add 13.8 μL of thioglycolic acid to 10 mL of ultrapure water, and then add 100 mg of EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, Mw=191.70) to activate 0.5-3h, then add 50mg polyethyleneimine dissolved in 15mL water dropwise while stirring, after reacting for 1-2 days, dialyze the reaction product in PBS buffer solution with dialysis membrane (MWCO=8000-14000) for 2 days, then Then dialyze with distilled water for 2 days, and finally freeze-dry the purified product to obtain partially thiol-modified polyet...

Embodiment 2

[0053] During the synthesis process, the obtained product pD@AuNSs-PEI of Example 1 was characterized by ultraviolet absorption as follows: figure 2 , the surface plasmon resonance (SPR) peak of gold seeds is about 520nm ( figure 2 a), Demonstrating the formation of gold seeds. pD@AuNSs-PEI has a broad plasmon resonance absorption peak in the range of 600 to 900 nm, demonstrating the successful synthesis of gold nanostar particles coated with polydopamine and stabilized by polyethyleneimine, and its application It offers possibilities in near-infrared imaging and photothermal therapy. The AuNSs-PEI and pD@AuNSs-PEI obtained in Step 4 and Step 5 in Example 1 were centrifuged at high speed (6000-7000r / min) for 3 times, redispersed in ultrapure water, and freeze-dried. Take 2 mg of freeze-dried PEI-SH, AuNSs-PEI, and pD@AuNSs-PEI respectively, and compress them with potassium bromide for infrared spectroscopic analysis. By comparing the infrared spectra of PEI-SH, AuNSs-PEI,...

Embodiment 3

[0055] Take 5 μL of the pD@AuNSs-PEI (Example 1) nanoparticle aqueous solution prepared in the present invention, and then prepare 100 μL of nanoparticle suspension with ultrapure water. Drop 5 μL of the nanoparticle suspension on the surface of the copper grid and dry it in the air for TEM testing (see Figure 4 ). TEM results show that pD@AuNSs-PEI has a star-shaped morphology, uniform particle size distribution, and a particle size of about 49.1nm. High-resolution TEM shows that the surface of the nanoparticles is covered with an organic layer, which is polydopamine and polyethylene. imine.

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Abstract

The invention relates to a preparation method for polydopamine-coated polyethyleneimine-stablized gold-nanometer-star photothermal treatment agent. The preparation method comprises the following steps: preparing a gold seed solution from chloroauric acid and sodium citrate; activating mercaptoacetic acid with EDC, then adding an aqueous PEI solution and carrying out a reaction, dialysis, cooling and drying so as to obtain PEI-SH; adding dopamine hydrochloride into a Tris buffer so as to obtain dopamine Tris buffer; adding the gold seed solution into a chloroauric acid solution, adding a AgNO3 solution and an ascorbic acid solution under stirring, adding PEI-SH after stirring reaction and carrying out a reaction so as to obtain AuNSs-PEI; and adding AuNSs-PEI into the dopamine Tris buffer and carrying out stirring and centrifugation so as to obtain the photothermal treatment agent. The preparation method has the advantages of simplicity, mild reaction conditions, easy operation and industrialization prospects; and the prepared star-like nanometer agent has application potential in the fields of CT imaging diagnosis and photothermal treatment of cancers.

Description

technical field [0001] The invention belongs to the field of preparation of photothermal therapeutic agents, in particular to a preparation method of a polydopamine-wrapped polyethylenimine-stabilized gold nano-star photothermal therapeutic agent. Background technique [0002] Cancer has become one of the main killers that endanger human health all over the world. Studies have shown that the cure of cancer is closely related to the early detection. The earlier the detection is, the higher the cure rate is. Therefore, early diagnosis of cancer is the key to overcoming cancer treatment. Clinically, computed tomography (CT) imaging technology has high spatial and density resolution, so it has been widely used and has become a commonly used diagnostic tool. In terms of cancer diagnosis and treatment, an ideal nanoparticle system that can be used for early diagnosis and treatment of cancer cells should have both imaging diagnostic and therapeutic functions, and can also be effic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/34A61K47/32A61K9/51A61K49/04A61P35/00
Inventor 史向阳李杜温诗辉
Owner DONGHUA UNIV
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