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Anti-tumor nano-medicine based on hierarchical release and preparation method thereof

A nano-drug and anti-tumor technology, used in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of low penetration into the depths of tumors and easy to be removed, and achieve good biocompatibility, no reagents. Poison, easy to prepare effect

Inactive Publication Date: 2019-06-21
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nano-drug carriers are widely used in targeted tumor therapy due to their enhanced penetration and retention effects. However, although nano-drug carriers with large sizes can highly accumulate at tumor sites, their penetration into tumor depths is very low, while small Although the size of the nano-drug carrier has good permeability, it is extremely easy to be removed during the delivery process

Method used

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  • Anti-tumor nano-medicine based on hierarchical release and preparation method thereof
  • Anti-tumor nano-medicine based on hierarchical release and preparation method thereof
  • Anti-tumor nano-medicine based on hierarchical release and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Dissolve 3mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station), 30mg soy lecithin (purchased from Shenyang Tianfeng Biological Co., Ltd.), and 1 mg paclitaxel (purchased from Shanghai Yuanye Biological Co., Ltd.) in 3 mL of absolute ethanol , Ultrasonically dissolve it, and drop it into 8mL distilled water drop by drop, magnetically stir for 1 hour to obtain paclitaxel nanoliposomes; add 5μL of 200mM sodium borohydride solution to 1.0mL of 0.5mM gold chloride solution, Shake vigorously to prepare a gold nanoparticle solution; mix 400 μL of each gold nanoparticle solution and paclitaxel nanoliposomes, add 300 μL of 1.5 mM gold chloride solution and 30 μL of 100 mM hydroxylamine hydrochloride, and incubate for 8 hours to obtain branches Paclitaxel nanoliposomes modified with a gold spherical shell; 0.5 mg of dopamine was placed in 5 mL of Tris-HCl buffer solution with a pH of 8.5 to polymerize the polydopamine solution, and then 1 mL of dopamine ...

Embodiment 2

[0041] Dissolve 4mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station), 40mg soybean lecithin (purchased from Shenyang Tianfeng Biological Co., Ltd.) and 2mg paclitaxel (purchased from Shanghai Yuanye Biological Co., Ltd.) in 4 mL of absolute ethanol , Dissolve it by ultrasound, drop it into 9mL distilled water drop by drop, magnetically stir for 1.5 hours to obtain paclitaxel nanoliposomes; add 7.5μL of 225mM sodium borohydride solution to 1.5mL of 0.75mM gold chloride solution , Shake vigorously to prepare a gold nanoparticle solution; mix each 450μL of gold nanoparticle solution with paclitaxel nanoliposomes, add 400μL of 1.75mM gold chloride solution and 40μL of 150mM hydroxylamine hydrochloride, and incubate for 9 hours to obtain a fraction Dendritic gold spherical shell modified paclitaxel nanoliposomes; 0.75 mg of dopamine was placed in 7.5 mL of Tris-HCl buffer solution with a pH of 8.5 to polymerize to obtain a polydopamine solution, and then ...

Embodiment 3

[0044] Dissolve 5mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station), 50mg soybean lecithin (purchased from Shenyang Tianfeng Biological Co., Ltd.), and 3mg paclitaxel (purchased from Shanghai Yuanye Biological Co., Ltd.) in 5 mL of absolute ethanol , Drop by drop into 10mL of distilled water, and magnetically stir for 2 hours to obtain paclitaxel nanoliposomes; add 10μL of 250mM sodium borohydride solution to 2.0mL of 1.0mM gold chloride solution and shake vigorously to prepare gold nanoparticles Solution; mix 500 μL of each gold nanoparticle solution and paclitaxel nanoliposomes, add 500 μL of 2.0 mM gold chloride solution and 50 μL of 200 mM hydroxylamine hydrochloride, and incubate for 10 hours to obtain branched gold spherical shell modified paclitaxel nanoparticles Liposome; Place 1.0 mg of dopamine in 10 mL of Tris-HCl buffer solution with a pH of 8.5 to polymerize to obtain a polydopamine solution, and then add 2 mL of doxorubicin at a concen...

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Abstract

The invention discloses an anti-tumor nano-medicine based on hierarchical release and a preparation method thereof. The preparation method includes coating anti-tumor medicine paclitaxel with nano-liposome so as to obtain paclitaxel nano-liposome; mixing a gold nanoparticle solution with the paclitaxel nano-liposome, adding a gold chloride solution and hydroxylamine hydrochloride, and incubating so as to obtain branched gold-spherical-shell-modified paclitaxel nano-liposome; subjecting dopamine to polymerizing in a Tris-HCl buffer solution so as to obtain a polydopamine solution, and then adding doxorubicin to obtain a doxorubicin-loaded polydopamine solution; mixing the prepared branched gold-spherical-shell-modified paclitaxel nano-liposome with the doxorubicin-loaded polydopamine, incubating and centrifugally purifying so as to obtain the final nano-medicine. The anti-tumor nano-medicine prepared by the preparation method solves the problem of insufficient medicine delivery depth intraditional tumor treatment, combines a local treatment mode with a systemic treatment mode and shows excellent biological safety and tumor inhibiting capability.

Description

Technical field [0001] The invention relates to a nano medicine, in particular to an anti-tumor nano medicine based on hierarchical release and a preparation method. Background technique [0002] Cancer has always been one of the greatest threats to human health. Traditional treatment methods such as chemotherapy and radiotherapy cannot completely cure tumors. Nano drug carriers can be targeted to tumor sites due to their enhanced penetration and retention effects, which largely solves the problem of low drug delivery efficiency. In addition, the slow drug release and responsive release of nano carriers reduce the toxic side effects of drugs. At the same time, nano-medicine carriers are easy to modify some photosensitizers with high biocompatibility. Under external stimulation, photosensitizers can generate heat or active oxygen, which shows excellent prospects in photothermal and photodynamic therapy of tumors. It can work together with chemotherapeutic drugs, showing extremely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34A61K31/704A61K31/337A61K9/127A61K41/00A61P35/00
Inventor 高大威何雨初张旭武丛聪李磊骆丽垚
Owner YANSHAN UNIV
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