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Targeted ZIF-8-polydopamine prodrug nanoparticles and preparation method and application thereof

A technology of ZIF-8- and polydopamine, which is applied in the field of biomedicine, can solve the problems of single nano-drug structure and lack of active targeting, and achieve the effect of simple operation

Pending Publication Date: 2021-11-23
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the defects existing in the prior art, the present invention provides a targeted ZIF-8-polydopamine prodrug nanoparticle and its preparation method and application to solve the single structure and lack of active targeting of nano-drugs in the prior art The combination of chemotherapy and photothermal therapy, which enhances intracellular uptake, greatly improves the effect of anti-tumor therapy through the synergistic treatment of the two combined therapies

Method used

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  • Targeted ZIF-8-polydopamine prodrug nanoparticles and preparation method and application thereof
  • Targeted ZIF-8-polydopamine prodrug nanoparticles and preparation method and application thereof
  • Targeted ZIF-8-polydopamine prodrug nanoparticles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method targeting ZIF-8-polydopamine prodrug nanoparticles, such as figure 1 As shown, the specific steps are as follows:

[0039] (1) Dissolve 2.5mg dopamine-doxorubicin (DA-DOX), 8.5mg dopamine (DA) and 5.72mg zinc chloride in a mixed solvent of 160mL ethanol / deionized water (volume ratio 4:1), and then Add 0.3mL ammonia water dropwise to adjust the pH value of the solution to 8.0-8.5.

[0040]

[0041] (2) After stirring for 0.5 h, 80 mL of 2-methylimidazole (2.07 mg) in ethanol / deionized water mixed solution was added dropwise thereto, and the reaction was stirred for another 48 h.

[0042] (3) After the reaction, the solution was placed in a dialysis bag with a molecular weight of 3500, and dialyzed with 500 mL of deionized water for 2 days, and the deionized water was changed every 8 hours in the middle. The yield of dopamine prodrug nanoparticles (ZIF-8-polydopamine-doxorubicin) is 68.4%-71.2%.

[0043]

[0044] The dynamic light scattering ...

Embodiment 2

[0049] Example 2 The effect of targeting ZIF-8-polydopamine prodrug nanoparticles on cervical cancer cells

[0050] Targeting ZIF-8-polydopamine prodrug nanoparticles (ZP-DOX-FA), ZIF-8-polydopamine prodrug nanoparticles (ZP-DOX) and doxorubicin (DOX) prepared in Example 1 Cell culture medium was used to prepare doxorubicin concentrations of 0.1, 0.2, 0.5, 1, 2, and 4 μg / mL, respectively, and then cultured with HeLa cells (cervical cancer adenocarcinoma) for 48 hours. In addition, for targeting ZIF-8- The polydopamine prodrug nanoparticles and ZIF-8-polydopamine prodrug nanoparticles need to set up another group respectively. After culturing for 4 hours, they are illuminated with near-infrared laser for 5 minutes (808nm, 2W / cm 2 ), continue to culture for 48h.

[0051] The cell viability test was carried out by the MTT method, and the results were as follows: Image 6 shown. Image 6 In the figure, the ordinate represents the activity of HeLa cells, and the lower abscissa D...

Embodiment 3

[0053] Example 3 Effect of Targeting ZIF-8-polydopamine Prodrug Nanoparticles on HeLa Tumor Growth

[0054] The mice inoculated with HeLa tumors were divided into 5 groups: normal saline, doxorubicin (5 mg / kg), ZIF-8-polydopamine prodrug nanoparticles (2 mg / mL) + NIR, targeting ZIF-8 - Polydopamine prodrug nanoparticles (2mg / mL), targeting ZIF-8-polydopamine prodrug nanoparticles (2mg / mL) + NIR. Inject once on the 0th day, and 12 hours after injection, ZIF-8-polydopamine prodrug nanoparticles+NIR and targeted ZIF-8-polydopamine prodrug nanoparticles+NIR were illuminated for 5min (808nm, 2W / cm 2 ), and the mice were weighed every other day and the tumor volume was measured, the results were as follows Figure 7 and 8 shown.

[0055] Figure 7 The middle ordinate represents the mouse tumor volume, Figure 8 The middle ordinate represents the body weight of the mice. Figure 7 , Figure 8 Among them, the abscissa indicates the number of days the mice received the experimen...

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Abstract

The invention discloses targeted ZIF-8-polydopamine prodrug nanoparticles and a preparation method and application thereof. The preparation method comprises the steps that dopamine-adriamycin, dopamine and zinc chloride are dissolved in a mixed solvent of ethanol / deionized water, then ammonia water is dropwise added, and the pH value of the solution is adjusted to 8.0-8.5; after a stirring reaction, an ethanol / deionized water mixed solution of 2-methylimidazole is dropwise added, the stirring reaction continues, and after the reaction is finished, the ZIF-8-polydopamine prodrug nanoparticles are obtained through dialysis; and finally, the ZIF-8-polydopamine prodrug nanoparticles and polyethylene glycol-folic acid are mixed and added into deionized water, and after the stirring reaction, the targeted ZIF-8-polydopamine prodrug nanoparticles are obtained through dialysis. The targeted ZIF-8-polydopamine prodrug nanoparticles and the preparation method and application thereof are used for solving the problems that in the prior art, a nano-drug is single in structure and lacks active targeting, and the problems of chemotherapy and photothermal therapy combined treatment and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a nanoparticle targeting ZIF-8-polydopamine prodrug and its preparation method and application. Background technique [0002] In recent years, cancer has overtaken heart disease as the leading cause of death facing humans worldwide. Therefore, the development of simple and efficient anticancer strategies is a major technical problem that needs to be solved urgently. At present, clinical chemotherapy is one of the most convenient and widely used effective cancer treatment methods, and the most widely used anticancer drug is doxorubicin. However, chemotherapy often has the risk of killing normal cells, destroying the immune system, and increasing the incidence of secondary cancers. Photothermal therapy, as an efficient new technology for cancer treatment, although highly selective and minimally invasive, is also subject to intermittent Clinical application problem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/704A61K47/69A61P35/00
CPCA61K41/0052A61K31/704A61K47/6949A61K47/6929A61P35/00A61K2300/00
Inventor 丁月马宇轩王陈威朱吕明
Owner NANTONG UNIVERSITY
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