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Virus-like hollow manganese oxide loaded near-infrared two-b region excited rare earth nanocrystal as well as preparation method and application thereof

A nanocrystal and rare earth technology, applied in the field of biomedical nanomaterials, can solve the problems of fluorescence tissue penetration, signal-to-noise ratio limitation, etc., and achieve the effects of high tissue penetration, high resolution, and strong tissue penetration.

Active Publication Date: 2022-01-14
MENGCHAO HEPATOBILIARY HOSPITAL OF FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of fluorescence tissue penetration and signal-to-noise ratio of NIR I, it is not suitable for fluorescence imaging of tumors located in deep organs, such as brain tumors, ovarian tumors, liver tumors, and lymphatic metastases.

Method used

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  • Virus-like hollow manganese oxide loaded near-infrared two-b region excited rare earth nanocrystal as well as preparation method and application thereof
  • Virus-like hollow manganese oxide loaded near-infrared two-b region excited rare earth nanocrystal as well as preparation method and application thereof
  • Virus-like hollow manganese oxide loaded near-infrared two-b region excited rare earth nanocrystal as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] β-NaErF 4 :2% Ho@NaYF 4 Preparation of core-shell nanocrystals:

[0126] First, Er(CH with a molar ratio of 49:1 3 CO 2 ) 3 4H 2 O(408.123mg), Ho(CH 3 CO 2 ) 3 4H 2 O (9.000 mg), a total of 1 mmol of rare earth raw materials, 6 mL of oleic acid, and 15 mL of octadecene were sequentially added to a 100 mL three-necked flask and mixed uniformly. Heated to 140 °C under vacuum and kept stirring for 30 minutes to remove water and oxygen in the system. Finally cooled to room temperature to obtain a clear and transparent solution. Dissolve 4mmol of ammonium fluoride and 2.5mmol of sodium hydroxide in 5mL of methanol respectively, mix quickly and add the above clear and transparent solution, keep stirring at 50°C for 1 hour to nucleate, then raise the temperature to 70°C, and Keep it down for 60min (to remove excess methanol, oxygen and water molecules). Subsequently, high-purity argon was introduced into the solution system while heating to 300° C. (heating rate 10°...

Embodiment 2

[0133] Preparation of virus-like hollow mesoporous manganese oxide particles loaded with IR1064:

[0134] (1) Synthesis of virus-like silicon mesoporous nanoparticles by two-phase method;

[0135] Add 60mL of ultrapure water and 1.5g of cetyltrimethylammonium bromide (CTAB) into a 100mL flask, dissolve and stir at 60°C for 0.5h, then add 0.75mL of 25% triethanolamine. After 0.5 h, a mixed solution of 16 mL cyclohexane and 4 mL tetraethyl orthosilicate was added, and after 48 h of reaction, the precipitate was obtained by centrifugation at 10,000 rpm for 10 min, and then washed three times with water and ethanol to obtain viroid silicon mesoporous nanoparticles.

[0136] (2) Preparation of virus-like hollow mesoporous manganese oxide

[0137] 100 mg of virus-like silicon mesoporous nanoparticles prepared in step (1) were ultrasonically dispersed in 50 mL of deionized water, and then 0.09 g of Mn(NO 3 ) 3 ·6H 2 O, and stirred in an oil bath at 90° C. for 0.5 h at 600 rpm. T...

Embodiment 3

[0142] Preparation of compound probes of rare earth nanocrystals excited by near-infrared 2b region excitation of IR1064-loaded hollow virus manganese oxide surface:

[0143] (1) β-NaErF wrapped with oleic acid prepared in Example 1 4 :2% Ho@NaYF 4 Rare earth nanocrystals (0.1 mmol) were dispersed in 5 mL of chloroform, and then 1 mL containing 25 mg of DSEP-PEG was added 2000 -COOH in chloroform. After the mixed solution was stirred in a glass bottle for 24 hours, the chloroform evaporated spontaneously in the air atmosphere. After the chloroform evaporated, put the glass bottle in an oven at 50°C for 0.5h to promote the further evaporation of chloroform. Finally, 5 mL of deionized water was added to the above-mentioned hydrophilic carboxyl phospholipid-modified rare earth nanocrystalline particles, and the excess carboxyl phospholipid was washed at least three times by an ultracentrifuge (17500 rpm, 30 min) by ultrasonication. Some large aggregates present after centrifu...

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Abstract

The invention discloses a virus-like hollow oxide loaded near-infrared two-b region excited rare earth nanocrystal and a preparation method and application thereof. According to the nanocrystal and the preparation method, an Er and Ho doped NaErF4:2%Ho@NaYF4 rare earth nanomaterial is synthesized through a hydrothermal method, then the nanomaterial is modified on a surface of a virus-like hollow manganese oxide poured into IR1064 through an amidation reaction, and a fluorescent composite probe which is good in biocompatibility and responds to a tumor microenvironment is obtained. According to the fluorescent composite probe, after the manganese oxide is degraded at a tumor, manganese ions are used for chemodynamic therapy and nuclear magnetic resonance imaging contrast agents of metastasis, released rare earth nanocrystals have excitation of 1530 nm and emitted lights of 650 nm and 1180 nm, the light of 1180 nm can be used for tumor imaging navigation surgical resection with no background interference and high resolution and tissue penetrating power in a near-infrared second region, and the light of 650 nm can used for near-infrared first region fluorescence imaging.

Description

technical field [0001] The invention belongs to the field of biomedical nanomaterials, and in particular relates to a rare-earth nanocrystal excited by a near-infrared 2b region supported by a virus-like hollow manganese oxide, a preparation method thereof, and an application for surgical navigation and postoperative chemokinetic therapy. Background technique [0002] Among the current treatment methods for cancer, complete and complete surgical resection is the most common and ideal method of choice. However, due to the limitation of its own resolution in the current routine preoperative examination methods for tumors, it is difficult to effectively judge and find the location, margin and micrometastasis of the tumor, and the remaining tumor cells after surgical resection will lead to postoperative tumor progression in patients. relapse. Accordingly, there is a need for an efficient imaging method for real-time, accurate, objective and precise positioning of the tumor loca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F1/07B82Y30/00B82Y40/00G01N21/64
CPCB82Y30/00B82Y40/00G01N21/6428G01N21/6486G01N2021/6432
Inventor 刘小龙王培园吴名李佳琦李阳蔺金燕罗强
Owner MENGCHAO HEPATOBILIARY HOSPITAL OF FUJIAN MEDICAL UNIV
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