35results about How to "Achieving Photodynamic Therapy" patented technology

The invention relates to a hypoxia response polymer nanoparticle and application thereof. The method comprises the following steps: firstly crosslinking 4,4-diaminoazobenzene and terephthalaldehyde taken as monomers, so as to form a conjugated polymer link, loading a photosensitizer and chemotherapy drugs through noncovalent interaction, and then forming the hypoxia response polymer nanoparticle through a nanoprecipitation method. The hypoxia response polymer nanoparticle can successfully carry the photosensitizer and the chemotherapy drugs to tumor cells, and produce active oxygen during illumination, so as to realize tumor killing. Azo bonds contained in the nanoparticle can be degraded under the action of reductase in cells, and chemotherapy drug release is realized. In addition, cell hypoxia caused during a photodynamic therapy process can further improve drug release, and the hypoxia response polymer nanoparticle can properly realize excellent tumor therapy effect through combination therapy effect of photodynamic therapy and chemical therapy.

The invention discloses a ternary complex nanodrug, a preparation method and application thereof in preparation of a light controlled release nano-delivery system. The ternary complex nanodrug comprises a thioketal bond crosslinked low molecular weight PEI-based polymer, a hyaluronic acid polymer attached with a photosensitizer, and a nucleic acid drug. The photosensitizer contained in the complexcan generate reactive oxygen species (ROS) under the control of exogenous red light, realize photochemical internalization (PCI) mediated lysosome escape and photopromoted polymer degradation, thus finally realizing the purposes of reducing the toxic and side effects of materials, improving the transfection efficiency, and enhancing the tumor gene therapeutic effect.

The invention relates to a preparation method of a composite magnetic nano photosensitizer. The preparation method includes preparing Fe3O4 magnetic nanoparticles, using tetraethoxysilane as a modifier to coat the surface of the Fe3O4 magnetic nanoparticles with a thin layer of silica, and fitting quantum dots CdT and a photosensitizer on the surface under the adsorbing action of silica mesoporousmicrospheres to obtain magnetic nano photosensitizer particles Fe3O4@SiO2@Qdots/PS. Specific bioluminescence of tumor cells can be used as a light source; the photosensitizer is excited to generate active oxygen clusters and convert electricity energy of an alternating electric field into thermal energy; the composite magnetic nano photosensitizer is a novel multifunctional photosensitizer.

The invention discloses a virus-like hollow oxide loaded near-infrared two-b region excited rare earth nanocrystal and a preparation method and application thereof. According to the nanocrystal and the preparation method, an Er and Ho doped NaErF4:2%Ho@NaYF4 rare earth nanomaterial is synthesized through a hydrothermal method, then the nanomaterial is modified on a surface of a virus-like hollow manganese oxide poured into IR1064 through an amidation reaction, and a fluorescent composite probe which is good in biocompatibility and responds to a tumor microenvironment is obtained. According to the fluorescent composite probe, after the manganese oxide is degraded at a tumor, manganese ions are used for chemodynamic therapy and nuclear magnetic resonance imaging contrast agents of metastasis, released rare earth nanocrystals have excitation of 1530 nm and emitted lights of 650 nm and 1180 nm, the light of 1180 nm can be used for tumor imaging navigation surgical resection with no background interference and high resolution and tissue penetrating power in a near-infrared second region, and the light of 650 nm can used for near-infrared first region fluorescence imaging.

The invention relates to application of an Yb<3+>-based fluoride nanocrystal as a photosensitizer in a photodynamic therapy and belongs to the field of the photodynamic therapy. According to the application, the problem of poor tissue penetration depth of exciting light caused by the wavelength of the exciting light of the photosensitizer in a visible region rang in the traditional photodynamic therapy is solved. The Yb<3+>-based fluoride nanocrystal is used as the photosensitizer to be applied to the photodynamic therapy. A preparation method of the Yb<3+>-based fluoride nanocrystal comprises the following steps: (1) adding YbCl3.6H2O into oleic acid and octadecene; (2) heating and dissolving; (3) adding NaOH and NH4F into methanol; (4) heating to react after mixing and water removal; (5) collecting NaYbF4 nanocrystals; (6) carrying out bond linking with Tween; and (7) carrying out bond linking with folic acid. According to the application, by irradiating by virtue of a light source with the wavelength of about 1 micron, deep tissue solid tumors can be treated by virtue of the wavelength in a biological tissue penetration window range.

The invention discloses polyurethane and a preparation method of polyurethane and ROS (Reactive Oxygen Species)-responsive polyurethane drug-loading micelles. The method comprises the following steps:dissolving hydrophobic drugs and prepared polyurethane solids into a dimethyl sulfoxide solution, slowly dropwise adding the solution into deionized water while intense stirring, continuously stirring, filling in a dialysis bag of MW=3500 after stirring completion, dialyzing for two days, dialyzing to remove non-entrapped hydrophobic drugs; and finally obtaining the stable ROS-responsive polyurethane drug-loading micelles with near-infrared light photothermal therapy and drug synergism. The preparation method of the polyurethane drug-loading micelles disclosed by the invention is simple and novel, and compared with the novel photosensitizer-ruthenium complex for photothermal therapy and the reported drug carrier before, the carrier disclosed by the invention is stable before subjected tonear-infrared stimulation when bonded into the main polymer chain, and effective release of the drugs and treatment of tumor cells can be realized once the carrier is initiated.

The invention belongs to the technical field of biomedical nano materials, and relates to preparation of a tumor microenvironment response type nano assembly and application of the tumor microenvironment response type nano assembly in tumor combined treatment. According to the nano assembly, bovine serum albumin stable gold nanoclusters (BSA and AuNCs) serve as carriers, nano particles loaded with manganese dioxide (MnO2) and indocyanine green (ICG) are prepared in a biomineralization and hydrophobic acting force mode, and the surfaces of the nano particles are combined with amino modified AS1411 (BSA and AuNCs-MnO2-ICG/AS1411, AMIT for short). By utilizing the tumor microenvironment responsiveness of MnO2 and the tumor targeting property of AS1411, the enrichment degree of a nano system at a tumor part is improved; under 808nm laser irradiation, the nano system can realize O2 self-replenishment enhanced photodynamic therapy effect, stable photothermal effect and Mn < 2 + > and endogenous H2O2 triggered chemical kinetics therapy effect, and shows excellent tumor combined therapy capability.

The invention discloses diiodostyrene type boron fluoride dipyrrole-hyaluronic acid as well as a preparation method and an application thereof. A hydrophobic photosensitizer diiodostyrene type boron fluoride dipyrrole with poor biocompatibility and poor targeting performance is connected onto hyaluronic acid to obtain an amphipathic molecule which is self-assembled to obtain a nano particle of the diiodostyrene type boron fluoride dipyrrole-hyaluronic acid; in addition to good water solubility, biocompatibility and CD44 special targeting performance, the nano particle further has the following advantages that firstly, passive targeting performance is realized, the accuracy of introducing the photosensitizer to a tumor part is improved, and tumor photodynamic therapy is realized; secondly, the intelligent monitoring for judging whether the photosensitizer nano particle accurately reaches the tumor part is realized; thirdly, after the nano particle enters the cells, the nano particle is self-assembled and dissociated, reactive oxygen species are recovered under illumination, the generation of the reactive oxygen species is intelligently controlled, and the defect that dosage for clinical tumor photodynamic therapy needs to keep in a dark place is solved.

The invention discloses a carrier capable of inhibiting tumor multidrug resistance and a preparation method thereof. The preparation method includes: allowing PEG to have reaction with 3,3'-thiodi(propionic acid) to prepare double-terminal-carboxylated PEG, adding targeting molecules to prepare targeting molecule-PEG, adding pyrene to prepare the targeting molecule-PEG-pyrene amphiphilic polymer carrier. The prepared targeting molecule-PEG-pyrene amphiphilic polymer carrier has the advantages that the carrier can form polymer polymeric micelles in water through self-assembling, the micelles can achieving efficient loading through the hydrophobic effect of the pyrene and drug molecules and pi-pi conjugate action, high glutathione concentration in tumor cells promote pyrene molecules to falloff when the micelles enter the tumor cells, the micelles disintegrate to accelerate drug release, the free pyrene molecules form nano aggregation states through self-assembling again in an in-situ manner in the cells under the hydrophobic effect and the pi-pi conjugate action, the pyrene molecules in the nano aggregation states can generate active oxygen under the stimulation of external light,and treatment integrating phototherapy and chemotherapy is achieved.

The invention discloses charge reversal type supramolecular polypeptide prodrug nanoparticles, a preparation method thereof and application of the charge reversal type supramolecular polypeptide prodrug nanoparticles in preparation of tumor treatment drugs, and belongs to the technical field of biological medicines. The charge reversal type supramolecular polypeptide prodrug nanoparticles are prepared by loading dihydroporphin on column [5] arene-poly (L-lysine) and pyridine-poly (L-lysine). After the nanoparticles enter tumor cells, under the irradiation of 660 nm near-infrared light, the loaded dihydroporphin can release active oxygen, so that the tumor cells are killed, and photodynamic therapy is realized; and meanwhile, the active oxygen can break a copper mercaptan link bond linked with adriamycin, so that free chemotherapeutic drug adriamycin is released, tumor cells are killed, and chemotherapy is carried out.

The invention discloses a bacterial carrier used for photodynamic therapy, and a preparation method and application of the bacterial carrier. Non-pathogenic Escherichia coli is used as a carrier to load a photosensitizer, a photodynamic therapy method is combined to realize the photodynamic therapy of tumor and realize delivery of the photosensitizer, the photosensitizer intake of tumor cells is significantly improved, the sensitivity of the focus to the photodynamic therapy method is increased, a treatment effect can be obviously improved, and the biological safety is good.

The invention relates to a preparation method of a rare earth upconversion composite nanomaterial, a product and application. The preparation method comprises the following steps that 1, sodium trifluoroacetate, yttrium trifluoroacetate, ytterbium trifluoroacetate and erbium trifluoroacetate are added into mixed liquid of octadecene and oleic acid; water removal and degassing treatment is performed to obtain reaction liquid; 2, then, the reaction liquid is added into the mixed liquid of octadecene and oleic acid at the temperature of 270 to 320 DEG C for reaction; after the separation, the rare earth upconversion nanomaterial is obtained; 3, the rare earth upconversion nanomaterial is subjected to assembly and modification of an amphiphilic macromolecular photosensitizer by an emulsified solvent volatilization method; the rare earth upconversion composite nanomaterial is obtained. The composite material can respond and disintegrate under the stimulation of tumor microenvironment; underthe irradiation of near-infrared light with high tissue penetrating force and through the absorption by the upconversion nanomaterial, a visible light excited photosensitizer molecule is formed through conversion; the photodynamic reaction is generated for killing tumor cells; the selective photodynamic therapy on deep tumor is realized.

The invention discloses a metal organic framework coated nano-particle capable of automatically supplying H2O2/O2 and consuming GSH and a preparation method and application of the metal organic framework coated nano-particle. The metal organic framework coated nano-particle is a core-shell structure compound and comprises a calcium peroxide nano-particle inner core, a calcium peroxide nano-particle outer core and a metal organic framework coated nano-particle inner core, the periphery of the calcium peroxide nanoparticle core is coated with the metal organic framework shell, and pores of the metal organic framework are loaded with a photosensitizer; and the phase change material is modified on the peripheral surface of the metal organic framework shell. The prepared nano-particles automatically supply H2O2/O2 and consume GSH, can effectively enhance the PDT/CDT curative effect, can be combined with chemotherapy drugs to amplify chemotherapy, avoids multi-drug resistance, and realizes multi-mode synergistic therapy of tumor photothermal therapy, photodynamic therapy, chemical dynamic therapy and chemotherapy.

The invention discloses a preparation method and application of a water-soluble ultrathin carbon nitride two-dimensional nanosheet, and the preparation method comprises the following steps: calcining a nitrogen-containing precursor at 550 DEG C to obtain initial blocky carbon nitride; stripping the initial blocky carbon nitride in a mixed acid solution of concentrated sulfuric acid and concentrated nitric acid to obtain porous carbon nitride; performing long-time ultrasonic stripping reaction on the porous carbon nitride to obtain a final product. The method is simple in preparation process, low in cost and good in repeatability. The nanosheet provided by the invention has a good fluorescence imaging function and can be used for dyeing cells; the nanosheet provided by the invention can generate a large amount of active oxygen under an illumination condition, so that the function of photodynamic therapy is realized; the nanosheet provided by the invention can be used as a photoresponsive non-viral gene vector, so that the delivery of DNA is promoted, and cell nucleuses can be targeted. The nanosheet provided by the invention can load a P53 gene and can realize gene and photodynamic combined therapy on tumors.

The invention discloses a near-infrared two-photon photosensitive dye SBOPI as well as a preparation method and application thereof. The near-infrared two-photon photosensitive dye SBOPI comprises a push-pull electronic conjugated structure of thiophene-boron dipyrromethene and indole and a lysosome targeting site. The preparation method of the near-infrared two-photon photosensitive dye SBOPI comprises the step that the near-infrared two-photon photosensitive dye SBOPI is prepared by reacting iodo thiophene-boron dipyrromethene SBOP with 1-(2-morpholinoethyl)-1H-indole-3-formaldehyde. According to the application of the near-infrared two-photon photosensitive dye SBOPI, under irradiation of near-infrared light, the near-infrared two-photon photosensitive dye SBOPI can efficiently releasesinglet oxygen, inhibit tumor cell migration and promote tumor cell apoptosis. As a near-infrared band two-photon photosensitizer and fluorescent dye with excellent performance, the near-infrared bandtwo-photon photosensitizer has wide application in the aspects of near-infrared band fluorescence imaging, fluorescence sensing, fluorescence labeling and two-photon photodynamic therapy.