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Tumor microenvironment response type nano assembly and application thereof in tumor combined treatment

A technology of nano-assembly and tumor microenvironment, which is applied in the combined treatment of tumors, and the field of preparation of tumor microenvironment-responsive nano-assembly, which can solve the problems that treatment methods cannot achieve good therapeutic effects, and achieve significant combination therapy Effect, simple preparation method, good stability

Pending Publication Date: 2022-03-18
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nowadays, a single treatment method cannot achieve a good therapeutic effect. Therefore, the idea of ​​integrating multiple treatment methods into one platform and building a multimodal combined treatment system has received a lot of attention

Method used

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  • Tumor microenvironment response type nano assembly and application thereof in tumor combined treatment
  • Tumor microenvironment response type nano assembly and application thereof in tumor combined treatment
  • Tumor microenvironment response type nano assembly and application thereof in tumor combined treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Nanosystem BSA@AuNCs-MnO 2 - Preparation of ICG / AS1411

[0043] (1) Preparation of bovine serum albumin-stabilized gold nanoclusters (BSA@AuNCs) with near-infrared fluorescence properties. BSA solution at a concentration of 50 mg / mL was mixed with HAuCl at a concentration of 10 mM 4 The solution was magnetically stirred and mixed at 37°C according to the volume ratio of 1:1, and NaOH solution was added to adjust the pH to 12, and the stirring was continued for 10-12 h to obtain a dark brown aqueous solution of gold nanoclusters. The aqueous solution was transferred into a dialysis bag (8KD-14KD) and dialyzed with deionized water for 12-15 h, and then stored at 4°C in the dark for future use.

[0044] (2) Add 0.1 M KMnO 4 The aqueous solution and 2 mg / mL ICG aqueous solution were sequentially added to the AuNCs aqueous solution obtained in step (1), and stirred vigorously for 3 h in the dark at room temperature, and the nanoparticle BSA@AuNCs-MnO was obtained through ...

Embodiment 2

[0047] Nanosystem BSA@AuNCs-MnO 2 - Preparation of ICG / AS1411

[0048] (1) Preparation of bovine serum albumin-stabilized gold nanoclusters (BSA@AuNCs) with near-infrared fluorescence properties. BSA solution at a concentration of 50 mg / mL was mixed with HAuCl at a concentration of 10 mM 4 The solution was magnetically stirred and mixed at 37°C according to the volume ratio of 1:1, and NaOH solution was added to adjust the pH to 12, and the stirring was continued for 10-12 h to obtain a dark brown aqueous solution of gold nanoclusters. The aqueous solution was transferred into a dialysis bag (8KD-14KD) and dialyzed with deionized water for 12-15 h, and then stored at 4°C in the dark for future use.

[0049] (2) Add 0.1 M KMnO 4 The aqueous solution and 2 mg / mL ICG aqueous solution were sequentially added to the AuNCs aqueous solution obtained in step (1), and stirred vigorously for 3 h in the dark at room temperature, and the nanoparticle BSA@AuNCs-MnO was obtained through ...

Embodiment 3

[0052] Nanosystem BSA@AuNCs-MnO 2 - Preparation of ICG / AS1411

[0053] (1) Preparation of bovine serum albumin-stabilized gold nanoclusters (BSA@AuNCs) with near-infrared fluorescence properties. BSA solution at a concentration of 50 mg / mL was mixed with HAuCl at a concentration of 10 mM 4 The solution was magnetically stirred and mixed at 37°C according to the volume ratio of 1:1, and NaOH solution was added to adjust the pH to 12, and the stirring was continued for 10-12 h to obtain a dark brown aqueous solution of gold nanoclusters. The aqueous solution was transferred into a dialysis bag (8KD-14KD) and dialyzed with deionized water for 12-15 h, and then stored at 4°C in the dark for future use.

[0054] (2) Add 0.1 M KMnO 4 The aqueous solution and 2 mg / mL ICG aqueous solution were sequentially added to the AuNCs aqueous solution obtained in step (1), and stirred vigorously for 3 h in the dark at room temperature, and the nanoparticle BSA@AuNCs-MnO was obtained through ...

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Abstract

The invention belongs to the technical field of biomedical nano materials, and relates to preparation of a tumor microenvironment response type nano assembly and application of the tumor microenvironment response type nano assembly in tumor combined treatment. According to the nano assembly, bovine serum albumin stable gold nanoclusters (BSA and AuNCs) serve as carriers, nano particles loaded with manganese dioxide (MnO2) and indocyanine green (ICG) are prepared in a biomineralization and hydrophobic acting force mode, and the surfaces of the nano particles are combined with amino modified AS1411 (BSA and AuNCs-MnO2-ICG / AS1411, AMIT for short). By utilizing the tumor microenvironment responsiveness of MnO2 and the tumor targeting property of AS1411, the enrichment degree of a nano system at a tumor part is improved; under 808nm laser irradiation, the nano system can realize O2 self-replenishment enhanced photodynamic therapy effect, stable photothermal effect and Mn < 2 + > and endogenous H2O2 triggered chemical kinetics therapy effect, and shows excellent tumor combined therapy capability.

Description

technical field [0001] The invention belongs to the field of medical nanomaterials, and in particular relates to the preparation of a tumor microenvironment-responsive nano assembly and its application in combined treatment of tumors. Background technique [0002] So far, cancer is still one of the most dangerous diseases in the world, and traditional cancer treatment methods, including surgery, chemotherapy, and radiotherapy, still have defects such as high recurrence, high trauma, and low treatment efficiency. With the development of nanotechnology, in recent years, a variety of mild non-invasive and spatiotemporal controllable treatment methods have attracted widespread attention due to their high efficacy and small side effects, such as photodynamic therapy (PDT), chemodynamic therapy (CDT) and photothermal therapy (PTT). PDT is the use of photosensitizers to absorb energy under specific wavelength laser irradiation to be excited, and further transfer the energy to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/32A61K47/54A61K47/69A61P35/00B82Y5/00B82Y30/00B82Y40/00G01K11/00
CPCA61K41/0052A61K41/0057A61K47/6929A61K47/549A61K33/32A61P35/00B82Y5/00B82Y30/00B82Y40/00G01K11/00A61K2300/00
Inventor 王慧敏孙玉东杨昌英王逸飞冉家兵
Owner CHINA THREE GORGES UNIV
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