33results about How to "Improve escape ability" patented technology

Provided are methods and compositions for obtaining functionally enhanced derivative effector cells obtained from directed differentiation of genomically engineered iPSCs. The derivative cells provided herein have stable and functional genome editing that delivers improved or enhanced therapeutic effects. Also provided are therapeutic compositions and the used thereof comprising the functionally enhanced derivative effector cells alone, or with antibodies or checkpoint inhibitors in combination therapies.

The invention discloses a genetic vector system of a nanoparticle with multiple oxidation-reduction stimulus response as well as a preparation method and application of the genetic vector system. The genetic vector system is composed of a shielding system, a cation material and foreign plasmid DNA (deoxyribonucleic acid); and the shielding system and the cation material both have oxidation-reduction stimulus response, so that a ternary complex nanoparticle with multiple oxidation-reduction stimulus response is formed. The genetic vector of the ternary complex nanoparticle is low in cytotoxicity, and capable of well compressing and compounding the plasmid DNA under physiological conditions, successfully transferring a supported genetic substance into a cell and entering the cell; and the reduction environment in the cell can be more rapidly broken to release the supported gene, so that the expression of gene substances is realized, the transfection process is finished, and the gene transfection efficiency can be remarkably increased.

The invention relates to the technical field of ocean platforms, in particular to an ocean platform with an escape device. The ocean platform comprises a salvage box, a hinged door, threaded hole blocks, an escape box, a mounting plate, a mounting ring, a locking motor and an escape motor, the hinged door is hinged to the salvage box, the two threaded hole blocks are fixedly connected to the salvage box, the mounting plate is fixedly connected to the escape box, the mounting ring is fixedly connected to the lower portion of the escape box, the inner side of the mounting ring is matched with the outer side of the salvage box, two locking motors are fixedly connected to the escape box, output shafts of the two locking motors penetrate through the lower portion of the escape box, locking studs are fixedly connected to the output shafts of the two locking motors, the two locking studs are in threaded transmission connection with the two threaded hole blocks correspondingly, the escape motor is installed on the lower portion of the mounting plate, and a plurality of shifting plates are evenly and fixedly connected to the axis of an output shaft of the escape motor in the circumferential direction. The ocean platform has the escape assisting function and further has the rescue and salvage function.

The invention discloses a star-shaped multi-targeting functional gene carrier based on POSS and its application. The carrier is made by the following method: octaamino POSS reacts with 2-bromoisobutyryl bromide to generate a macromolecular initiator POSS-(Br )n, through two-step atom transfer polymerization, the hydrophilic dimethylaminoethyl methacrylate and poly(ethylene glycol) methacrylate are grafted on POSS, and double bonds are introduced at the end of the polymer, and finally , modify the targeting functional polypeptide to the polymer to obtain a star-shaped multi-targeting functional gene carrier based on POSS, which can be used to prepare a gene delivery system. The star-shaped structure of the gene carrier of the present invention can make the positive charge more Concentration, thereby enhancing the ability of the polymer to load genes; it is easier to form stable micelles with smaller particle sizes, which is more conducive to cell uptake. Using the PDMAEMA chain segment as the main chain segment to load the compression gene can enhance the endosome escape ability.

The invention discloses a multi-targeted modified gene-loaded compound, a preparation method and application. The method comprises the following steps: preparing a membrane targeted peptide modified polycation gene vector (I); evenly mixing a TAT-NLS polypeptide aqueous solution and a nucleic acid aqueous solution to obtain mixed liquor, and preparing the gene vector (I) into suspension liquid; and (4) evenly mixing the suspension liquid and the mixed liquor, and standing to obtain the multi-targeted modified gene-loaded compound. The multi-targeted modified gene-loaded compound is specifically recognized by REDV peptide in membrane targeted peptide and an integrin receptor on the surface of an endothelial cell, and uptake of the cell to the compound is improved. The compound which enters the cell is positioned in a lysosome / endosome structure, polyethyleneimine and TAT jointly act, the escaping capacity of the endosome of the compound is improved, and a target gene is promoted to enter cytoplasm. Through interaction of a nuclear localization signal NLS and a nuclear membrane, nuclear internalization of the target gene is promoted. Transfection efficiency of the target gene in the endothelial cells is high.

The invention relates to a degradable nanocomposite coating loaded with miRNAs, a preparation method and application thereof. The construction method includes the following steps: S1: construction of miRNAs-loaded nanoparticles; S2: modification of miRNAs-loaded nanoparticles; S3: deposition and thiolation of polydopamine coating; S4: miRNAs-loaded nanoparticles / hyaluronic acid hydrogel Preparation of adhesive composite coatings. Through the construction of the coating, the invention can realize the controlled release and intracellular delivery of miRNAs-loaded nanoparticles, selectively inhibit blood coagulation reaction and excessive proliferation of smooth muscle, promote the growth of endothelial cells, and effectively improve the biocompatibility of materials.

The invention discloses a gene vector system of a redox sensitive shielding system having targeting function, its preparation and application in the field of gene therapy. The gene vector system disclosed herein comprises a redox sensitive shielding system having targeting function, a cationic high-molecular material and plasmid DNA; wherein the cationic high-molecular material and plasmid DNA form compound particles, the redox sensitive shielding system having targeting function shields the compound surface by electrostatic action, thus the toxicity of the vector can be reduced, the loaded genetic material is transferred into the cell successfully, the expression of the genetic material is realized, the transfection process is completed, the targeting of gene transfection can be raised, and simultaneously the gene transfection efficiency is raised.

The invention provides a kind of chitosan cross-linked gel and its preparation method and application, the method utilizes chitosan and imidazole-4,5-dicarboxylate dimethyl to prepare imidazole cross-linked chitosan, and adopts Coated with hyaluronic acid, the gel has strong proton buffering capacity and drug loading function, and has CD44 targeting, which can be used as drug excipients and has broad application prospects.

The invention discloses a glass lifer and a vehicle with the same. The glass lifter comprises an electric motor, a hand-cranking device, a planetary gear mechanism, a lifting driving assembly and a switching device; a planet gear is arranged on a planet carrier and meshes between a sun gear and a gear ring; two of the sun gear, the planet carrier and the gear ring respectively serve as a first input end and a second input end and the remaining one serves as an output end; the first input end is connected with the electric motor; the second input end is connected with the hand -cranking device; the lifting driving assembly is connected with the output end and glass so as to drive glass to rise and fall; and the switching device is arranged for unlocking or locking the hand-cranking device so as to realize power input switching between the electric motor and the hand-cranking device. Therefore, vehicle door glass can be provided with two driving modes of electric driving and hand cranking; and in a dangerous state, a driver and passengers can open vehicle windows by the hand-cranking device to escape, thereby improving the escaping capabilities of the driver and the passengers.

A dual-strap backpack-type individual high-rise escape apparatus, characterized in that: the individual high-rise escape apparatus is packed in a dual-strap backpack; an individual high-rise escape apparatus rear housing (71) is provided on the left and right sides respectively with one waistband ring (92), each waistband ring (92) is connected to the rear housing (71), a waistband (6) is fixed on or run through the waistband rings (92); the dual-strap backpack is provided on both the left and right sides respectively with one waistband hole, the left and right extremities of the waistband (6) run through the waistband holes of the corresponding sides; a steel-wire cable hole (22) is also provided on the upper part of the dual-strap backpack, and the outer extremity of a steel-wire cable of the individual high-rise escape apparatus is run through the steel-wire cable hole (22) and then tied to a hook (3), that is, the hook (3) is outside of the dual-strap backpack. The positive effects of the apparatus are: a transmission mechanism of the individual high-rise escape apparatus is in a sealed state, thus preventing other impurities from entering the transmission mechanism, and significantly increasing the reliability of escape; secondly, it can be equipped with an auxiliary apparatus for the escape apparatus, thus increasing escape effect.

The invention relates to the technical field of safety of vehicles, and provides a door opening assisting device for a vehicle and a control method of the device. The device comprises a pressure sensor, a door switching determining module, a control module and at least one pair of magnetic elements; two magnetic elements in each pair of magnetic elements are respectively oppositely arranged on a door and a vehicle frame; the door switching detecting module is used for detecting the state of the handle on the door and sends an opening signal to the control module when detecting that the door handle is pulled; the pressure sensor is used for detecting the water pressure value borne by the door and sends the water pressure value to the control module; when determining that the water pressure value is more than the preset value and the opening signal is received synchronously, the control module controls the two magnetic elements of each pair of magnetic elements to generate interaction repulsive to assist to open the door. According to the device, the control module controls the magnetic elements to produce a magnetic field which assists to open the door, and therefore, a user can easily pull and open the door in case of falling into deep water, and as a result, the escape capacity of the user is improved, and the safety performance of the vehicle is improved.

The present invention relates to the technical field of offshore platforms, more specifically an offshore platform with an escape device, including a salvage box, a hinged door, a threaded hole block, an escape box, a mounting plate, a mounting ring, a locking motor and an escape motor. A hinged door is hingedly connected, two threaded hole blocks are fixedly connected to the salvage box, a mounting plate is fixedly connected to the escape box, and a mounting ring is fixedly connected to the lower part of the escape box. There are two locking motors fixedly connected, the output shafts of the two locking motors run through the lower part of the escape box, the output shafts of the two locking motors are fixedly connected with locking studs, and the two locking studs are respectively connected with The two threaded hole blocks are connected by screw transmission, the escape motor is installed on the lower part of the mounting plate, and the output shaft axis of the escape motor is evenly fixed and connected with a plurality of toggle plates in the circumferential direction. The present invention has an auxiliary escape function. Further, the present invention has rescue Salvage function.

The invention discloses a ternary complex nanodrug, a preparation method and application thereof in preparation of a light controlled release nano-delivery system. The ternary complex nanodrug comprises a thioketal bond crosslinked low molecular weight PEI-based polymer, a hyaluronic acid polymer attached with a photosensitizer, and a nucleic acid drug. The photosensitizer contained in the complexcan generate reactive oxygen species (ROS) under the control of exogenous red light, realize photochemical internalization (PCI) mediated lysosome escape and photopromoted polymer degradation, thus finally realizing the purposes of reducing the toxic and side effects of materials, improving the transfection efficiency, and enhancing the tumor gene therapeutic effect.