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A mesoporous silica nanoparticle with two-photon absorption function and its preparation method and application

A technology of mesoporous silica and two-photon absorption, which is applied in the field of biological applications of nanomaterials, can solve the problems of unsatisfactory dispersion and small particle size, and achieve the effects of mild conditions, low cost, and simple preparation methods

Active Publication Date: 2019-01-15
北京柏欧利诺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the invention has small particle size, biocompatibility and biodegradability, the dispersibility is not ideal enough

Method used

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  • A mesoporous silica nanoparticle with two-photon absorption function and its preparation method and application
  • A mesoporous silica nanoparticle with two-photon absorption function and its preparation method and application
  • A mesoporous silica nanoparticle with two-photon absorption function and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] In this example, the following method is used to prepare a pharmaceutical composition of mesoporous silica nanoparticles with two-photon absorption function:

[0067] (1) Take 200 μL of methanol solution of amino-functionalized polyglycidyl ether (the degree of amino-functionalization of PG-NH2 is 30%, and the concentration is 3 mg / mL), add 2000 μL of sodium carbonate buffer solution with pH=8, stir well, and then add 1 mg 100 μL of dimethylformamide (DMF) solution of FITC / mL, protected from light and stirred at a speed of 1500 rpm for 24 h;

[0068] (2) Purification of FITC-PG: Add the above reaction solution into a dialysis bag with a molecular permeability of 3000 Da, dialyze the clear solution with pure water for 24 hours, freeze-dry it at -40°C for 30 hours, add 2 mL of methanol to dissolve it, and obtain FITC - PG methanol stock solution;

[0069] (3) Adsorption of TEOS: Take 600 μL of the above-mentioned FITC-PG methanol stock solution, add 200 μL TEOS (PG-NH cr...

Embodiment 2

[0090] In this example, the following method is used to prepare a pharmaceutical composition of mesoporous silica nanoparticles with two-photon absorption function:

[0091] (1) Take 200 μL of ethanol solution of amino-functionalized polyglycidyl ether (the degree of amino-functionalization of PG-NH2 is 1%, and the concentration is 3 mg / mL), add 2000 μL of sodium carbonate buffer solution with pH=7.1, stir well, and then add 1 mg 60 μL of dimethylformamide (DMF) solution of FITC / mL, protected from light and stirred at a speed of 100 rpm for 48 h;

[0092] (2) Purification of FITC-PG: Add the above reaction solution into a dialysis bag with a molecular permeability of 3000 Da, dialyze the clear solution with pure water for 24 hours, freeze-dry it at -80°C for 12 hours, add 2 mL of ethanol to dissolve it, and obtain FITC - PG ethanol stock solution;

[0093] (3) Adsorption of TEOS: Take 600 μL of the above-mentioned FITC-PG ethanol stock solution, add 200 μL of TEOS (PG-NH cros...

Embodiment 3

[0096] In this example, the following method is used to prepare a pharmaceutical composition of mesoporous silica nanoparticles with two-photon absorption function:

[0097] (1) Take 200 μL of dimethyl sulfoxide solution of amino-functionalized polyglycidyl ether (the degree of amino-functionalization of PG-NH2 is 70%, and the concentration is 3 mg / mL), add 2000 μL of sodium carbonate buffer solution with pH=10, After stirring evenly, 300 μL of 1 mg / mL FITC in dimethylformamide (DMF) solution was added, protected from light, and stirred at 3000 rpm for 1 h;

[0098] (2) Purification of FITC-PG: Add the above reaction solution into a dialysis bag with a molecular permeability of 10,000 Da, dialyze the clear solution with pure water for 24 hours, freeze-dry it at -60°C for 48 hours, and add 2 mL of dimethyl sulfoxide Dissolve to obtain FITC-PG dimethyl sulfoxide stock solution;

[0099] (3) Adsorption of TEOS: Take 600 μL of the above FITC-PG dimethyl sulfoxide stock solution, ...

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Abstract

The invention relates to a mesoporous silica nanoparticle having a two-photon absorption function and a preparation method and application thereof. The preparation method includes: cross-linking amino-functionalized polyglycerol with fluorescein, performing purifying to obtain fluorescein-modified polyglycerol, absorbing silica precursor, and performing hydrolysis, solid-liquid separation, washing and drying to obtain the mesoporous silica nanoparticle having the two-photon absorption function. The invention further provides a pharmaceutical composition prepared with the mesoporous silica nanoparticle; the pharmaceutical composition comprises a photosensitive pharmaceutical carried on the mesoporous silica nanoparticle. The preparation method is simple, conditions are mild, the cost is low, and no toxic or harmful matters occur to a reaction process; the prepared nanoparticle has regular shape, moderate pore diameter density and particle size and good dispersity and enables fluorescence to rarely quench; the pharmaceutical enables resonance transfer of fluorescence energy under stimulation of two photons and enters deep in in-vivo tumor tissues for treatment.

Description

technical field [0001] The invention belongs to the field of biological applications of nanomaterials, in particular to a mesoporous silica nanoparticle and its preparation method and application, in particular to a mesoporous silica nanoparticle with two-photon absorption function and its preparation method and application application. Background technique [0002] Photodynamic therapy is a new type of cancer treatment that uses laser light to irradiate photosensitizing drugs to generate reactive oxygen molecules, which interfere with the growth of tumor cells and lead to their apoptosis. Compared with traditional radiotherapy and chemotherapy, this method effectively avoids the damage of drugs to normal tissue cells and reduces the physical burden of cancer patients during treatment. However, since tumor tissues are generally located inside the human body, and the photosensitizing drugs of photodynamic therapy that have been clinically approved can only effectively absorb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/04A61P35/00
Inventor 杨洋刘惠玲韩明娟王安河李峻柏
Owner 北京柏欧利诺生物科技有限公司
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