The invention discloses a nano-carrier for
drug and
gene co-delivery and a preparation method and application thereof, and particularly relates to a co-delivery nano-carrier TCPL-siRNA-PPX for simultaneously carrying a chemotherapeutic
drug and a
gene-based
drug. A
polymeric prodrug carrier TCPL, siRNA and a multifunctional polyanionic
polymer PPX are self-assembled by
electrostatic adsorption between ingredients to form the
drug delivery system; a drug and a
gene can be targeted to be delivered to the same tumor
cell, and moreover, siRNA is released in
cytoplasm to
silence the Bc1-2
protein, promoting
apoptosis and releasing the inhibition of Bcl-2 on
lonidamine; the chemotherapeutic drug acting on mitochondria,
lonidamine, is delivered to the mitochondria; both cooperatively trigger the
apoptosis of the mitochondrial pathway, jointly killing the tumor
cell. By in-vitro and in-vivo activity evaluation, the invention proves that the
system is better than the simultaneous delivery of each
single component, can remarkably increase anti-
cancer activity, and has a definite synergistic
treatment effect.