143results about How to "Improve tumor treatment effect" patented technology

The invention relates to a portable high-voltage nanosecond squarer which belongs to a medicinal device for inducing cancer cell apoptosis by using a high-voltage nanosecond square wave pulse electric field. The portable high-voltage nanosecond squarer mainly comprises a power supply system, a high-voltage direct current module, a low-voltage power supply, a pulse forming system and a pulse shaping and counting system. The portable high-voltage nanosecond squarer has output pulses with highest amplitude of 15kV, pulse width adjustment range of 50ns-1us, rising edge gradient of reaching 10ns and controllable recurrence frequency of 0.2-15Hz; and the quantity of the output pulses of the portable high-voltage nanosecond squarer can be set. The portable high-voltage nanosecond squarer can be adjusted in multiple-parameters (pulse amplitude, pulse width, recurrence frequency, pulse quantity and the like), large-range and flexible and independent way, and is beneficial to researching the optimal window parameter of the cancer cell apoptosis and promoting the cancer cell apoptosis as well as improving the cancer treatment effect. Meanwhile, the portable high-voltage nanosecond squarer has compact and light structure, reliable work, simple operation and convenient popularization and application, and can be widely applied to the treatment of various cancers.

The invention discloses an ultra-small MoS2 nanosheet. The surface of the MoS2 nanosheet is modified with polyvinylpyrrolidone, and the diameter of the modified MoS2 nanosheet ranges from 15 nm to 25 nm. The invention further discloses a preparation method of the ultra-small MoS2 nanosheet. The preparation method comprises the following steps: a sulfur source and a molybdenum source are dissolved in water and stirred until the resources are completely dissolved; polyvinylpyrrolidone is dissolved in the solution of the sulfur source and the molybdenum source; the solution is transferred into a stainless steel reaction kettle with a polyphenyl lining for closed reaction, a reaction product is washed with an ethanolamine water solution and distilled water, and the ultra-small MoS2 nanosheet with the surface modified with polyvinylpyrrolidone is obtained. The invention further provides an application of the ultra-small MoS2 nanosheet as a photo-thermal conversion material. The ultra-small MoS2 nanosheet has good colloid stability, photo-thermal conversion capacity and photo-acoustic imaging capacity and has broad application prospect in the fields of tumor treatment and the like.

The invention provides a composite gold nanorod carrier having photo-thermal / photodynamic therapy treatment performance and a preparation method thereof. The composite gold nanorod carrier uses a gold nanorod as a core and uses silicon dioxide as a shell layer, a near infrared fluorescent dye for photodynamic therapy is modified on the outer surface of the shell layer. The preparation method comprises the steps that a chloroauric acid solution and a cetyl trimethyl ammonium bromide solution are mixed, a sodium borohydride ice water mixed solution is added to obtain a nano gold seed solution A; a silver nitrate solution and a chloroauric acid solution are added to a binary surface active agent solution, a hydrochloric acid is added after reaction to obtain a solution B, then ascorbic acid and the solution A are added, and a gold nanorod is obtained after reaction; a tetraethoxysilane methanol solution and an aminopropyl trimethoxy silane methanol solution are added to a gold nanorod solution to obtain composite nanoparticles; 1-(3-dimethyl amino propyl)-3-ethyl carbodiimide hydrochloride is added to the near infrared fluorescent dye, then N-hydroxyl succinimide is added to obtain a solution C; the composite nanoparticle solution is mixed with the solution C to obtain a product.

The invention discloses a mesoporous polydopamine loaded manganese carbonyl multifunctional nano diagnosis and treatment agent and a preparation method and an application thereof. The multifunctionalnano diagnosis and treatment agent is a water-soluble mesoporous polydopamine carrier-coated hydrophobic manganese carbonyl drug, wherein the mass ratio of mesoporous polydopamine to manganese carbonyl is 1-8:1. The multifunctional nano diagnosis and treatment agent realizes gas and photo-thermal collaborative treatment under MRI and photoacoustic imaging guiding, is hopeful for increasing tumourtreatment effect, has good biocompatibility, and has clinical application potential.

The invention discloses a co-delivery nano-carrier of drugs and genes, a preparation method and an application thereof; and particularly relates to a co-delivery nano-carrier TCPL-siRNA-PPX which can carry a chemotherapeutic drug and a genetic drug simultaneously. The drug delivery system is composed of a polymer prodrug carrier TCPL, siRNA and a multifunctional polyanionic polyer PPX through electrostatic adsorption among the components in a self-assembly manner. The co-delivery nano-carrier can achieve targetedly delivery of the drug and the gene to the same tumor cell, release the siRNA at the cytoplasm, silence Bcl-2 protein, promote apotosis and relieve inhibition of the Bcl-2 on lonidamine, and can deliver the chemotherapeutic drug, lonidamine, which is mitochondrion-acted to mitochondria so that the chemotherapeutic drug and the gene can synergistically trigger the apotosis of a mitochondrial pathway and kill tumor cells cooperatively. Through in-vivo and in-vitro activity evaluation, the drug delivery system is proved to be better than drug delivery carriers which deliver single components respectively at the same time, can significantly improve the anti-cancer activity of the drug and the gene and has a definite synergistic treatment effect.

The invention relates to a hypoxia response polymer nanoparticle and application thereof. The method comprises the following steps: firstly crosslinking 4,4-diaminoazobenzene and terephthalaldehyde taken as monomers, so as to form a conjugated polymer link, loading a photosensitizer and chemotherapy drugs through noncovalent interaction, and then forming the hypoxia response polymer nanoparticle through a nanoprecipitation method. The hypoxia response polymer nanoparticle can successfully carry the photosensitizer and the chemotherapy drugs to tumor cells, and produce active oxygen during illumination, so as to realize tumor killing. Azo bonds contained in the nanoparticle can be degraded under the action of reductase in cells, and chemotherapy drug release is realized. In addition, cell hypoxia caused during a photodynamic therapy process can further improve drug release, and the hypoxia response polymer nanoparticle can properly realize excellent tumor therapy effect through combination therapy effect of photodynamic therapy and chemical therapy.

The invention discloses an acid-responsive nanometer micelle for drug loading, a preparation method and an application thereof. The nano-micelles were self-assembled from hydrophilic segment polyethylene glycol (PEG) and hydrophobic segment pH-sensitive polyaspartyl diisopropylethylenediamine / di-n-butylethylenediamine (PAsp (DIP / DBA)). The nano-micelles can prolong the drug circulation time, aggregate in the tumor site, increase the local drug concentration, and respond to the micro-acid environment of the tumor tissue. As a drug carrier of stimulation response, the nano-micelles can rapidly release the loaded chemotherapeutic drug adriamycin in the tumor site, and play a significant anti-tumor effect. At the same time, the nano-micelles loaded with magnetic resonance contrast agent superparamagnetic iron oxide can be used for tumor magnetic resonance imaging and monitoring drug uptake and aggregation. This method utilizes nano-drug aggregation at tumor site and acid responsiveness ofcarrier to realize rapid drug release to improve tumor therapeutic effect, and endows nano-micellar MRI visualization function, which provides a promising innovative strategy for cancer diagnosis andtreatment, and has broad application prospects.

The invention discloses a porphyrin-based metal organic framework nano-carrier with a sonodynamic combined starvation therapy anti-tumor function and a preparation method of the porphyrin-based metal organic framework nano-carrier. The preparation method comprises the following steps: dissolving a porphyrin-based ligand and metal salt in a solvent, and heating to obtain porphyrin-based metal organic framework particles; and dispersing the porphyrin-based metal organic framework particles into water, adding high-valence metal acid or high-valence metal salt and a reducing agent, and mixing with a cell membrane and glucose oxidase to obtain the cell membrane modified porphyrin-based metal organic framework nano-carrier loaded with the metal nanoparticles and the glucose oxidase. The nano-carrier can enhance the accumulation at the tumor site and improve the tumor targeted hunger treatment effect; and meanwhile, tumor hypoxia is relieved, sonodynamic reaction substrates are increased, and the sonodynamic effect is improved. The preparation process is simple and controllable, the material source is wide, and scale amplification can be realized; and in vivo, good stability and tumor sonodynamic and hunger treatment combined treatment effect are achieved, and good application prospects are achieved in the field of tumor treatment.

The invention provides a hollow manganese dioxide nano-particle as well as a preparation method and application thereof. The preparation method comprises: a silver nanocube is first prepared, and thenthe silver nanocube is used as a template, and the hollow manganese dioxide nano-particle is synthesized with a template method in a one-step process; the hollow manganese dioxide nano-particle has the characteristics of being simple in preparation process and the like; at the same time, the hollow manganese dioxide nano-particle, prepared with the method, has the advantages of stable properties,controllable size and structure and the like, and can be applied in the fields of drug carriers, tumor treatment, magnetic resonance imaging and the like.

The invention relates to a polypeptide and chemotherapy drug combined drug-loaded micelle and a preparation method and an application thereof. Polypeptide with a tumor targeting therapeutical effect and a chemotherapy drug are combined to prepare the tumor-targeting polypeptide-PEGylated phospholipid complex-chemotherapy drug drug-loaded micelle; the polypeptide with the tumor targeting therapeutical effect is specifically combined with a chemotactic factor receptor CXCR4, the CXCR4 polypeptide antagonist is used for increasing a targeting penetration ability of the drug to tumor tissues, the PEGylated phospholipid micelle is used for increasing the drug biological stability and the drug loading amount, and compared with a single chemotherapy drug doxorubicin, the combined drug-loaded micelle shows a stronger characteristic of inhibiting tumor cell vitality. The polypeptide and chemotherapy drug combined drug-loaded micelle provides a feasible method and technique for improving the tumor therapeutic effect.

The invention discloses a synergistically reinforced anti-tumor near-infrared photoresponsive NaYF4: Yb, Er-NH2 / RBS / GO / BNN6 nanocomposites, NaYF4: Yb, Er-NH2 layer, NO donor RBS layer, GO layer, NO donor BNN6 layer. The NaYF4: Yb, Er-NH2 / RBS / GO / BNN6 nanocomposites have good thermal stability and high near-infrared response. NO emission can be triggered and controlled effectively by the switching of near infrared laser, the adjustment of irradiation time and power density. NaYF4: Yb, Er and GO, NO donors RBS and BNN6 can play a synergistic role, greatly enhance the response to near-infrared light, can significantly improve the therapeutic effect of tumor, showing a synergistic enhancement of the anti-tumor effect.

The invention discloses a penetration-enhanced gold nanocluster drug-loaded targeting preparation and a preparation method and application thereof. The targeting preparation comprises gold nanoclusters, a cross-linking agent and a tumor treatment drug, wherein the gold nanoclusters are obtained by assembling gold nanospheres with the cross-linking agent, and the surface of the gold nanoclusters ismodified with the targeting tumor treatment drug of human epidermal growth factor receptor-2. The preparation can be used as an antitumor drug carrier, specifically targets to a tumor, enables the drug to be efficiently released at a tumor site through tumor microenvironment reduction and NIR irradiation, and improves the treatment effect in combination with thermal therapy and action. By the preparation, the advantages of gold in imaging aspects such as CT imaging and photoacoustic imaging (PA) can also be combined for medical diagnosis, and a purpose of multi-mode accurate tumor diagnosis and treatment is achieved.

The invention discloses a targeted nano carbon quantum dot carrier CQDs-PEG-Tf of an accessory immune cell therapy and a preparation method thereof and application of a carrier mediated antitumor drug an immune cells in the targeted tumor therapy. It is proved through in vitro experiments that the carrier can be combined with the tumor cell surface over-expression receptor specifically, and the good biological safety to immune cells is achieved by means of a carrier mediated antitumor drug under a certain concentration. The carrier mediated antitumor drug is combined with the immune cells to double target and kill tumor cells, the double anti-tumor effect is achieved, and toxic and side effects of the antitumor drug on normal cells can be reduced. The carrier is simple in preparation method and easy to carry out and has a high targeting property, nontoxicity, excellent biocompatibility and biodegradability. The carrier can be applicable to preparation of targeted anticancer drugs and the targeted therapy of accessory immune cells on various cancer diseases.

The invention discloses an acid-sensitive paclitaxel prodrug and a preparing method and an application thereof. The acid-sensitive paclitaxel prodrug has a structure which is shown in the first formula. According to the acid-sensitive paclitaxel prodrug, a polymer precursor is amphipathy two-inlayed-section copolymer of vinyl ether functionlization, and drug molecules are covalently linked to the amphipathy two-inlayed-section copolymer through acetal bonds. The paclitaxel prodrug can be subjected to self-assembly in an aqueous solution, so that prodrug nano-micelle is formed, wherein a polyethylene glycol hydrophilic segment is used as the outer surface, a lateral group is used for polycaprolactone functionlization, and antineoplastic drug paclitaxel which is linked to polymer main chains through the acetal bonds is used as a hydrophobic core. The drug loading capacity of the micelle is higher, and leakage and burst release of the drug can be avoided in the body circulation process. The polymer prodrug has the pH-sensitive acetal bonds, the drug cannot be released under the weak alkaline environment in normal cells, the drug is slowly released under the acid environment in tumour cells, the tumour cells can be killed in a specificity mode, and accordingly the toxic and side effects of the drug are lowered.

The invention discloses a tumor drug-resistant target site and application of the tumor drug-resistant target site. The invention discloses influence of NAC1 (Nucleus Accumbens-1) on tumor medicine treatment, and thus provides a purpose of an NAC1 inhibitor in medicine for reducing or eliminating tumor chemotherapy drug resistance and application of the NAC1 inhibitor as a tumor drug-resistant mark and a drug-resistant tumor prediction and diagnosis and drug-resistant tumor medicine design and screening target. The invention provides a novel mechanism for overcoming tumor drug resistance and improving the curative effect, and provides a novel target for resisting tumor drug resistance.

The invention discloses a liposome-gadolinium meso-porous silicon compound for cancer combined therapy and three kinds of imaging, and a preparation method thereof. The preparation method comprises the following steps: preparation of GdMSNs; preparation of DOX@GdMSNs; and preparation of DOX@GdMSNs-ICG-TSLs. The liposome-gadolinium meso-porous silicon compound DOX@GdMSNs-ICG-TSLs for cancer combined therapy and three kinds of imaging can be excited by a near-infrared light source, and has the advantages of realization of phototherapy and chemotherapy combined therapy, realization of fluorescence, photoacoustic and nuclear magnetic imaging integration, good biocompatibility, great improvement of the tumor treatment effect, reduction of toxic and side effects, and simplicity and easiness in preparation and use.

The invention discloses a nano diagnosis and treatment agent as well as a preparation method and an application thereof. The nano diagnosis and treatment agent is formed by self-assembling organic ligand molecules, Fe <2+> and amphiphilic molecules, wherein a structural formula of the organic ligand molecule is shown in the specification,-R is-CH3 or-CH2CH3, the novel organic ligand molecule designed by the invention can keep the stable valence state of Fe <2+>, Fe <2+> is delivered to a tumor site through a nano self-assembly technology, and a chemical kinetic treatment effect is generated. In addition, the organic ligand molecules also have near-infrared photothermal conversion capability, so that the photothermal therapy is realized, and meanwhile, the chemodynamic therapy effect is improved. The novel drug delivery mode provides a new strategy for tumor synergistic treatment.

The invention discloses a polymer nanocarrier capable of promoting endocytosis and cell nucleus targeting and a preparation method of the polymer nanocarrier. The polymer nanocarrier material is an amphipathic block polymer, a hydrophobic end is a polyester capable of being biologically degraded and absorbed, a hydrophilic chain segment is polyethylene glycol with a terminal linked with polypeptide capable of promoting endocytosis and cell nucleus targeting, the polypeptide is modified by a small molecule, and an amido bond, linked with the polypeptide, of the small molecule responds to a faintly acid environment of tumors, so that the endocytosis and the nucleus targeting drug delivery are promoted; and a cell nucleus can be targeted by virtue of a cell nucleus targeting signal. The polymer nanocarrier material and medicines can form medicine-carrying nanoparticles in water through self-assembling, and the preparation method is simple.

The invention provides a gynostemma pentaphylla saponin long-circulating liposome preparation and a preparation method thereof. The invention discloses a method for preparing gynostemma pentaphylla saponin long-circulating liposome after modification of beta-sitosterol by polyethylene glycol. Compared with common phosphatide polyethylene glycol modified long-circulating liposome, the gynostemma pentaphylla saponin long-circulating liposome prepared in the invention has the following advantages: material synthesis cost is greatly reduced, separation is easier, yield is substantially increased, and cost for the long-circulating liposome is substantially reduced. The novel long-circulating liposome has the characteristics of easy and wide availability of raw materials, a cheap price, good stability, capacity of substantially prolonging detention time of gynostemma pentaphylla saponin in a human body, realizing passive targeting of gynostemma pentaphylla saponin to tumor cells and improving tumor treatment effects and an excellent antihyperlipidemic effect.

The invention relates to an amorphous calcium carbonate composite nano-medicine with an effect of inducing ferroptosis of tumor cells and a preparation method of the amorphous calcium carbonate composite nano-medicine, and belongs to the technical field of medicines. After the composite nano-medicine enters the tumor cells, the degradation of nanoparticles is promoted by means of the acidic environment of lysosomes, meanwhile, the lysosomes are subjected to permeabilization to release doxorubicin and ferrous ions through the protonation of dendrimers, wherein ferrous ions can promote the conversion of polyunsaturated fatty acids of cell membranes to lipid peroxides and induce ferroptosis of the tumor cells, and DOX can promote oxidative stress to increase the content of hydrogen peroxide in the cells while inducing apoptosis, so that iron-dependent ferroptosis caused by lipid peroxidation induced by the ferrous irons is further enhanced. The composite nanoparticles can not only increase the therapeutic effect of tumors, but also reduce the side effects caused by chemotherapy.

The invention provides a medicine composition for treating tumors and an application thereof. The medicine composition is composed of acriflavine or pharmaceutically acceptable salts thereof, melphalan or pharmaceutically acceptable salts thereof, and a medicinal carrier, wherein the molar ratio of the acriflavine to the melphalan is 1:100 to 1:10, and the tumors comprise blood tumors and solid tumors. The forms of the medicine comprise single preparations prepared from acriflavine or melphalan respectively, or a compound preparation prepared from acriflavine and melphalan. The medicine composition provided by the invention has the effects of improving the curative effect on tumors, the toxicity of the medicine composition can be reduced, and the growth speeds of the tumors can be slowed down.

The invention relates to a portable high-voltage nanosecond squarer which belongs to a medicinal device for inducing cancer cell apoptosis by using a high-voltage nanosecond square wave pulse electric field. The portable high-voltage nanosecond squarer mainly comprises a power supply system, a high-voltage direct current module, a low-voltage power supply, a pulse forming system and a pulse shaping and counting system. The portable high-voltage nanosecond squarer has output pulses with highest amplitude of 15kV, pulse width adjustment range of 50ns-1us, rising edge gradient of reaching 10ns and controllable recurrence frequency of 0.2-15Hz; and the quantity of the output pulses of the portable high-voltage nanosecond squarer can be set. The portable high-voltage nanosecond squarer can be adjusted in multiple-parameters (pulse amplitude, pulse width, recurrence frequency, pulse quantity and the like), large-range and flexible and independent way, and is beneficial to researching the optimal window parameter of the cancer cell apoptosis and promoting the cancer cell apoptosis as well as improving the cancer treatment effect. Meanwhile, the portable high-voltage nanosecond squarer has compact and light structure, reliable work, simple operation and convenient popularization and application, and can be widely applied to the treatment of various cancers.

The invention relates to preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof. The preparation is mainly prepared from dipalmitoyl phosphatidylcholine (DPPC), hollow gold nanoparticles and therapeutic agents. A preparation method of the preparation comprises the following steps: preparing thermosensitive liposome via a conventional method, coating the thermosensitive liposome with the hollow gold nanoparticles by using a film extrusion method, and then coating the thermosensitive liposome with the therapeutic agents. The preparation is capable of reducing toxic and side effects of systemic drug therapy and is also capable of achieving the triple integration therapy effect with combination of drug therapy, thermal therapy and radiation therapy; the tumor killing effect is improved.

The invention discloses intestinal probiotics and application thereof in treating tumors and replacing antibiotics. The lactobacillus reuteri JMR-01 provided by the invention has the preservation number of CGMCC No. 21304. The invention also provides a complex microbial inoculant of the complex microbial inoculant and the following application of the complex microbial inoculant: 1) treatment and / or prevention of tumors; 2) inhibiting of tumor cell proliferation; 3) preventing or treating of diseases caused by pathogenic bacteria; 4) replacing of antibiotics; 5) inhibiting of proliferation of pathogenic bacteria; and 6) promoting or synergistical treating of tumors. The invention provides intestinal probiotics, a method for treating tumors based on the intestinal probiotics and improving the tumor treatment effect by assisting irradiation treatment, and a method for treating tumors by taking the intestinal probiotics as a raw material.

The invention relates to a preparation method, product and application of a molybdenum oxide nano material. The preparation method comprises the steps that 1, molybdenyl acetylacetonate is dissolved into a mixed solution of octadecene, oleic acid and hexadecylamine, and the materials are stirred and react at the temperature of 90-150 DEG C to generate a molybdenum oxide nano intermediate; 2, the mixed solution containing the molybdenum oxide nano intermediate in step 1 continues to be subjected to a hydrothermal reaction to obtain the urchin-like hollow molybdenum oxide nano material. The obtained molybdenum oxide nano material can achieve specific toxicity activation on tumor parts for efficient tumor imaging and treatment, and can also be rapidly degraded in normal tissue to ensure goodbiological safety.

The invention provides a new target for regulating and controlling tumor cell metastasis and an application thereof; the application is an application of a DcR3 inhibitor in preparation of medicines for reducing or eliminating tumor cell metastasis. The invention discloses a new mechanism of the DcR3 in regulation and control of tumor metastasis, and the DcR3 is clear and definite to be used as an anti-tumor migration target; through studies, the DcR3 is indicated to be capable of regulating and controlling expression of crucial molecule E-cadherins in an EMT generation process, promote tumor cytoskeleton remodeling, and then promote the migration of tumor cells. In general, the application can reduce or alleviate malignant tumor metastasis, thereby playing a role in improving the effect of tumor therapy.

The invention provides a preparation method of a cancer cell membrane chimeric liposome nano drug delivery system. The preparation method comprises the following steps of: dissolving high-purity cholesterol, hydrogenated soybean phospholipid, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and indometacin in absolute ethyl alcohol under a dark condition to prepare an ethanol solution; carrying out rotary evaporation on the obtained ethanol solution to form a thin film; hydrating the thin film to obtain a hydrated thin film; adding a cancer cell membrane suspension solution into the hydrated thin film and extruding to obtain chimeric indometacin liposome; mixing the chimeric indometacin liposome and ZSTK474 carrier-free nanoparticles; and extruding to obtain the cancer cell membrane chimeric liposome nano drug delivery system. By adopting the preparation method of the cancer cell membrane chimeric liposome nano drug delivery system, provided by the invention, an active targeting function on tumor tissues is increased, the anti-tumor efficiency of a drug is easy to improve and the dosage of a tumor cell membrane is extremely saved; and the preparation method is convenient for clinical application.

The invention relates to the field of virology and tumor therapy. In particular, the present invention provides a recombinant herpes simplex virus (HSV) capable of specifically replicating at a high level in a tumor cell and effectively killing the tumor cell, but replicating at low levels in normal cells, thereby the recombinant herpes simplex virus of the present invention not only has high lethality against tumor cells, but also has significantly decreased side effects (especially neurotoxicity). Further, the present invention relates to a viral vector constructed based on the recombinant herpes simplex virus, a pharmaceutical composition comprising the recombinant herpes simplex virus or the viral vector, and the use of the recombinant herpes simplex virus or the viral vector. The recombinant herpes simplex virus of the present invention can be used to infect and kill tumor cells, and can be used for gene drug delivery into tumor cells for gene therapy.

The invention relates to an anti-tumor drug and a preparation method thereof. The anti-tumor drug consists of the following components in parts by weight: 0.1-0.3 parts by weight of betulin, 20-30 parts by weight of dendrobium nobile and 5-10 parts by weight of ginseng. The preparation method of the anti-tumor drug comprises the following steps: crushing the dendrobium nobile into powder, so that dendrobium nobile powder is obtained; crushing the ginseng into powder, so that ginseng powder is obtained; and weighing 0.1-0.3 parts by weight of betulin, 20-30 parts by weight of the dendrobium nobile powder and 5-10 parts by weight of the ginseng powder, and uniformly mixing the materials, so that the anti-tumor drug is obtained. The anti-tumor drug and the preparation method thereof disclosed by the invention have the advantages of being good in therapeutic effect, rapid to take effects, simple in preparation method and the like.