Porphyrin-based metal organic framework nano-carrier with sonodynamic combined starvation therapy anti-tumor function and preparation method of porphyrin-based metal organic framework nano-carrier

An organic framework and starvation therapy technology, applied in the field of porphyrin-based metal organic framework nanocarriers and their preparation, can solve the problems of reducing the effect of sonodynamic therapy, less tumor accumulation of sonosensitizers, and low loading capacity.

Inactive Publication Date: 2021-07-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sonosensitizer is easily eliminated quickly in the body, the accumulation of tumor tissue is small, there is a self-quenching effect, and the sonodynamic effect is not satisfactory
In addition, the hypoxic microenvironment at the tumor site cannot provide enough oxygen for sonodynamic therapy, which also reduces the effect of sonodynamic therapy in tumor killing.
[0003] At present, variou

Method used

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  • Porphyrin-based metal organic framework nano-carrier with sonodynamic combined starvation therapy anti-tumor function and preparation method of porphyrin-based metal organic framework nano-carrier
  • Porphyrin-based metal organic framework nano-carrier with sonodynamic combined starvation therapy anti-tumor function and preparation method of porphyrin-based metal organic framework nano-carrier
  • Porphyrin-based metal organic framework nano-carrier with sonodynamic combined starvation therapy anti-tumor function and preparation method of porphyrin-based metal organic framework nano-carrier

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Tetracarboxyphenyl porphyrin and ZrOCl 2 Dissolve in DMF to make the concentrations 0.83mg / mL and 2.5mg / mL respectively, stir and react at 90°C for 5 hours, centrifuge to remove the supernatant after the reaction, add solvent to wash the particles, and centrifuge to remove the supernatant to obtain porphyrin metal-organic framework nanoparticles;

[0021] 2) Disperse the porphyrin-based metal-organic framework particles obtained in step 1) into water, add H 2 PtCl 6 The solution was adjusted to a concentration of 0.26 mg / mL, and stirred at a constant temperature of 25°C for 60 min. Add NaBH dropwise 4 , so that the concentration was 0.40 mg / mL, and stirred at a constant temperature of 25 ° C for 180 min. adding water to wash the particles, and centrifuging to remove the supernatant to obtain porphyrin-based metal-organic framework nanoparticles loaded with metal nanoparticles on the surface;

[0022] 3) Mix the porphyrin-based metal-organic framework nanoparticl...

Embodiment 2

[0024] Step is the same as Example 1, but in step 2) with RhCl 3 instead of H 2 PtCl 6 , successfully prepared porphyrin-based metal-organic framework nanocarriers.

Embodiment 3

[0026] The steps are the same as in Example 1, but in step 3), platelet cell membranes are used instead of red blood cell membranes to successfully prepare porphyrin-based metal organic framework nanocarriers.

[0027] The electron micrographs of porphyrin-based metal-organic framework nanoparticles and nanocarriers prepared by the method of the present invention are shown in figure 1 . and figure 1 a) Compared to, figure 1 b) The electron microscope picture of the surface loaded with metal nanoparticles can see granular substances; figure 1 In c), the cell membrane layer covering the surface can be seen, with a thickness of about 7 nm.

[0028] figure 2 is the reactivity of porphyrin-based metal-organic framework nanocarriers in solution. figure 2 In a), the nanocarriers loaded with metal nanoparticles (PPGE) produced more oxygen in hydrogen peroxide solution compared with the control group (PGE and blank), demonstrating the ability of metal nanoparticles to mimic cata...

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Abstract

The invention discloses a porphyrin-based metal organic framework nano-carrier with a sonodynamic combined starvation therapy anti-tumor function and a preparation method of the porphyrin-based metal organic framework nano-carrier. The preparation method comprises the following steps: dissolving a porphyrin-based ligand and metal salt in a solvent, and heating to obtain porphyrin-based metal organic framework particles; and dispersing the porphyrin-based metal organic framework particles into water, adding high-valence metal acid or high-valence metal salt and a reducing agent, and mixing with a cell membrane and glucose oxidase to obtain the cell membrane modified porphyrin-based metal organic framework nano-carrier loaded with the metal nanoparticles and the glucose oxidase. The nano-carrier can enhance the accumulation at the tumor site and improve the tumor targeted hunger treatment effect; and meanwhile, tumor hypoxia is relieved, sonodynamic reaction substrates are increased, and the sonodynamic effect is improved. The preparation process is simple and controllable, the material source is wide, and scale amplification can be realized; and in vivo, good stability and tumor sonodynamic and hunger treatment combined treatment effect are achieved, and good application prospects are achieved in the field of tumor treatment.

Description

technical field [0001] The invention belongs to the field of preparation of anti-tumor nanomaterials, and relates to a porphyrin-based metal-organic framework nano-carrier with the anti-tumor function of sonodynamic combined with starvation therapy and a preparation method thereof. Background technique [0002] Due to the increasing morbidity and mortality, tumor has become a major killer of human health. Excessive production of reactive oxygen species (ROS) in local tumors can cause apoptosis and necrosis of tumor tissue, and finally achieve the effect of tumor killing. Ultrasound-based sonodynamic therapy has the advantages of low cost, convenient use, deep penetration, and non-invasiveness, and has broad potential application prospects in tumor treatment. However, the sonosensitizer is easily eliminated in the body quickly, the accumulation of tumor tissue is small, there is a self-quenching effect, and the sonodynamic effect is not satisfactory. In addition, the hypoxi...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K38/44A61K9/50A61K47/46A61K47/69A61P35/00B82Y5/00A61K33/24A61K33/242A61K33/243
CPCA61K9/5068A61K33/24A61K38/443A61K41/0033A61K47/6949A61P35/00A61K33/242A61K33/243B82Y5/00C12Y101/03004A61K2300/00
Inventor 仝维鋆黄品同包宇衡陈继繁
Owner ZHEJIANG UNIV
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