Liposome-gadolinium meso-porous silicon compound for cancer combined therapy and three kinds of imaging, and preparation method thereof

A combined therapy and liposome technology, applied in the field of nano-biomedicine, can solve the problems of easy drug resistance, achieve the effect of simple preparation and application process, reduce toxic and side effects, and improve curative effect

Inactive Publication Date: 2018-04-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term single use of this chemotherapy drug is prone to drug resistance

Method used

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  • Liposome-gadolinium meso-porous silicon compound for cancer combined therapy and three kinds of imaging, and preparation method thereof
  • Liposome-gadolinium meso-porous silicon compound for cancer combined therapy and three kinds of imaging, and preparation method thereof
  • Liposome-gadolinium meso-porous silicon compound for cancer combined therapy and three kinds of imaging, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation method of the liposome-gadolinium mesoporous silicon complex used for combined cancer therapy and three kinds of imaging comprises the following steps:

[0051] (1) Preparation of GdMSNs: 0.015g of Gd 2 o 3 Add to 0.64mL, 2M NaOH aqueous solution, ultrasonic (100W) 1.5h, get Gd 2 o 3 / NaOH mixed solution; heat 100mL ultrapure water to 30°C, add 0.15g CTAB under stirring condition, stir for 30min, raise the temperature to 80°C, add the above 0.64mL Gd dropwise 2 o 3 / NaOH mixed solution, then add 0.75mL TEOS dropwise, stir for 2h, centrifuge at 7500rpm for 8min, wash the precipitate with absolute ethanol three times, add 40mL absolute ethanol to the washed precipitate, and reflux at 60°C for 36h , remove the template agent CTAB, separate the solid from the liquid, wash the solid three times with absolute ethanol, and centrifuge at 7500rpm for 8min each time to obtain GdMSNs, and the GdMSNs are dispersed in 10mL of water;

[0052] (2) Preparation of D...

Embodiment 2

[0055] The preparation method of the liposome-gadolinium mesoporous silicon complex used for combined cancer therapy and three kinds of imaging comprises the following steps:

[0056] (1) Preparation of GdMSNs: 0.02g of Gd 2 o 3 Add to 0.85mL, 2M NaOH aqueous solution, ultrasonic (100W) 2h, get Gd 2 o 3 / NaOH mixed solution; heat 100mL ultrapure water to 30°C, add 0.2g CTAB under stirring condition, stir for 35min, raise the temperature to 80°C, add the above 0.85mL Gd dropwise 2 o 3 / NaOH mixed solution, then add 1mL TEOS dropwise, stir for 2h, centrifuge at 8000rpm for 10min, wash the precipitate 4 times with absolute ethanol, add 50mL absolute ethanol to the washed precipitate, reflux at 65°C for 48h, Remove the template agent CTAB, separate the solid from the liquid, wash the solid with absolute ethanol 4 times, and centrifuge at 8000rpm for 10min each time to obtain GdMSNs, which are dispersed in 10mL of water;

[0057] (2) Preparation of DOX@GdMSNs: Take 6mL of the ...

Embodiment 3

[0060] The preparation method of the liposome-gadolinium mesoporous silicon complex used for combined cancer therapy and three kinds of imaging comprises the following steps:

[0061] (1) Preparation of GdMSNs: 0.025g of Gd 2 o 3 Add to 1.06mL, 2M NaOH aqueous solution, ultrasonic (100W) 2.5h, get Gd 2 o 3 / NaOH mixed solution; heat 125mL ultrapure water to 40°C, add 0.25g CTAB under stirring condition, stir for 40min, raise the temperature to 70°C, add the above 1.06mL Gd dropwise 2 o 3 / NaOH mixed solution, then add 1.25mL of TEOS dropwise, stir for 2.5h, centrifuge at 8500rpm for 12min, wash the precipitate with absolute ethanol 4 times, add 60mL of absolute ethanol to the washed precipitate, and reflux at 65°C 48h, remove the template agent CTAB, separate the solid from the liquid, wash the solid 4 times with absolute ethanol, and centrifuge at 8500rpm for 12min each time to obtain GdMSNs, which are dispersed in 10mL of water;

[0062] (2) Preparation of DOX@GdMSNs: T...

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Abstract

The invention discloses a liposome-gadolinium meso-porous silicon compound for cancer combined therapy and three kinds of imaging, and a preparation method thereof. The preparation method comprises the following steps: preparation of GdMSNs; preparation of DOX@GdMSNs; and preparation of DOX@GdMSNs-ICG-TSLs. The liposome-gadolinium meso-porous silicon compound DOX@GdMSNs-ICG-TSLs for cancer combined therapy and three kinds of imaging can be excited by a near-infrared light source, and has the advantages of realization of phototherapy and chemotherapy combined therapy, realization of fluorescence, photoacoustic and nuclear magnetic imaging integration, good biocompatibility, great improvement of the tumor treatment effect, reduction of toxic and side effects, and simplicity and easiness in preparation and use.

Description

technical field [0001] The invention belongs to the field of nano-biomedicine, and relates to a liposome-gadolinium mesoporous silicon complex used for combined cancer treatment and three imaging and a preparation method. Background technique [0002] The treatment of cancer has always attracted much attention. Photothermal therapy and photodynamic therapy are effective methods developed in recent decades for the non-surgical treatment of various malignant tumors, which have the advantages of minimal invasiveness and high selectivity. The combined treatment method consisting of phototherapy and chemotherapy overcomes the limitations of each other to a greater extent, and at the same time greatly improves the killing effect on tumors and reduces the occurrence of side effects. [0003] Fluorescence imaging is based on the fact that fluorescent substances are excited by specific external energy (such as near-infrared rays, etc.), causing their electronic orbits to transition ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K41/00A61K49/00A61K47/04A61K49/08A61K49/22A61P35/00
CPCA61K31/704A61K41/0057A61K47/02A61K49/0034A61K49/08A61K49/22A61K2300/00
Inventor 李楠孙琪游青王金平刘丽王怡丹宋宜霖程玉王思宇谭丰苹
Owner TIANJIN UNIV
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