Preparation method of cancer cell membrane chimeric liposome nano drug delivery system

Abstract

The invention provides a preparation method of a cancer cell membrane chimeric liposome nano drug delivery system. The preparation method comprises the following steps of: dissolving high-purity cholesterol, hydrogenated soybean phospholipid, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and indometacin in absolute ethyl alcohol under a dark condition to prepare an ethanol solution; carrying out rotary evaporation on the obtained ethanol solution to form a thin film; hydrating the thin film to obtain a hydrated thin film; adding a cancer cell membrane suspension solution into the hydrated thin film and extruding to obtain chimeric indometacin liposome; mixing the chimeric indometacin liposome and ZSTK474 carrier-free nanoparticles; and extruding to obtain the cancer cell membrane chimeric liposome nano drug delivery system. By adopting the preparation method of the cancer cell membrane chimeric liposome nano drug delivery system, provided by the invention, an active targeting function on tumor tissues is increased, the anti-tumor efficiency of a drug is easy to improve and the dosage of a tumor cell membrane is extremely saved; and the preparation method is convenient for clinical application.

Description

technical field [0001] The invention belongs to the technical fields of tumor targeted therapy, immunotherapy and targeted delivery, and in particular relates to a preparation method of a cancer cell membrane chimeric liposome nano drug delivery system. Background technique [0002] Tumor is one of the diseases with the highest fatality rate in the world. The occurrence of tumor is closely related to the excessive activation of PI3K signaling pathway in tumor cells. Therefore, targeting and inhibiting the PI3K signaling pathway of tumor cells can produce anti-tumor effect. The PI3K inhibitor ZSTK474 has shown good anti-tumor effect in preclinical animal experiments, but the effect of clinical trials is not satisfactory. This may be related to its distribution in other tissues other than the tumor, resulting in adverse reactions that lead to patients' intolerance and discontinuation of the drug, thereby affecting the curative effect. [0003] The non-steroidal anti-inflammat...

AI Technical Summary

Problems solved by technology

However, the source of cancer cell membrane is limited, which limits large-scale preparation and application

[0006] At present, the combination of molecular targeted anti-tumor therapy and immunotherapy, while directly killing tumor cells, while improving the microenvironment of tumor immunosuppression, there are few studies. The use of carrier-free nanoparticles and cell membrane chimeric drug-loaded liposome-modified nanoparticles delivery system has not been reported

Images