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Composite gold nanorod carrier having photo-thermal/photodynamic therapy treatment performance and preparation method thereof

A photodynamic therapy and gold nanorod technology, which is applied in the field of gold nanorod composite carrier and its preparation, can solve the problems affecting the application of near-infrared fluorescent dyes, low quantum yield, etc., and achieve synergistic enhancement of photothermal and photodynamic therapy effects, The effect of enhancing the effect of tumor treatment and significantly improving the effect of tumor treatment

Inactive Publication Date: 2017-01-04
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fluorescent dyes are prone to agglomeration in water, resulting in fluorescence quenching, and the quantum yield is low. These problems affect the application of near-infrared fluorescent dyes in photodynamic therapy.

Method used

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  • Composite gold nanorod carrier having photo-thermal/photodynamic therapy treatment performance and preparation method thereof
  • Composite gold nanorod carrier having photo-thermal/photodynamic therapy treatment performance and preparation method thereof
  • Composite gold nanorod carrier having photo-thermal/photodynamic therapy treatment performance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Mix 5mL 0.5mM chloroauric acid with 5mL 0.2M cetyltrimethylammonium bromide solution, stir well, add freshly prepared 0.6mL 0.01M NaBH 4 , stirred rapidly for 2 minutes, the color of the solution changed from yellow to brownish yellow, and the gold seed solution continued to grow for 30 minutes at room temperature.

[0039] Weigh 1.4g of cetyltrimethylammonium bromide and 0.2468g of sodium oleate, add 50ml of water, heat and stir to dissolve, cool to room temperature, add 4.8ml of 4nM silver nitrate and 50ml of 0.5mM chloroauric acid, and let stand for 15min , continue to stir for 1.5h, add 0.26ml, 0.35ml and 0.55ml of concentrated hydrochloric acid in parallel in three parts, stir for 15min, then add 0.25ml of 64mM ascorbic acid, stir rapidly for 30s, finally add 0.16ml of the gold seed solution prepared above, stir for 30s, 30s ℃ for 12 hours, centrifuged and washed, dispersed in water to obtain AuNR solutions with different aspect ratios (AR). TEM images of AuNR nan...

Embodiment 2

[0041] According to the steps of Example 1, by adjusting the feeding amount of hydrochloric acid to 0.27mL, such as figure 2 A shows AuNR nanoparticles with an aspect ratio of 3.3. The UV absorption spectrum of this AuNR nanoparticles is as follows image 3 Shown; AuNR nanoparticles have strong plasmon resonance absorption at 783nm. Take 10ml of the prepared AuNR solution, add 100l of 0.1M sodium hydroxide, stir for 15min, add 12μL of 10% tetraethylorthosilicate (TEOS) in methanol and 12μL of 1% aminopropyltrimethoxysilane (APTMS) in methanol , a total of three times, with an interval of 30min each time, stirred in 30°C water for 24h, centrifuged, washed and dispersed in water to obtain AuNR@SiO 2 solution. AuNR@SiO 2 TEM images of composite nanoparticles such as figure 2 As shown in B, an obvious silicon dioxide layer can be seen from the transmission electron microscope image, and its thickness is about 12.2nm. AuNR@SiO 2 The UV absorption spectrum of the nanopartic...

Embodiment 3

[0043] Take 2.5ml 4μg / ml cyanine dye (IR795) solution, add 15μL 1mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, stir at room temperature for 30min, then add 15μL 1mM N-hydroxysuccinimide was reacted for 30min. 10nM AuNR@SiO 2 Add the composite nanoparticles to the above solution, and continue to shake at room temperature in the dark for 5 hours. After the reaction, the suspension is centrifuged and washed twice, and the collected precipitate is redispersed in an equal volume of deionized water to obtain AuNR@SiO 2 - IR795 core shell nanoparticles. After connecting to IR795, the UV absorption spectrum and transmission electron microscope picture are consistent with AuNR@SiO 2 of no significant change. AuNR@SiO 2 The infrared spectrum of -IR795 composite nanocarrier is as Figure 4 As shown, after modifying IR795, 1657cm -1 The peak is enhanced, which is the characteristic peak of the amide bond, indicating that IR795 has been successfully connected to the...

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Abstract

The invention provides a composite gold nanorod carrier having photo-thermal / photodynamic therapy treatment performance and a preparation method thereof. The composite gold nanorod carrier uses a gold nanorod as a core and uses silicon dioxide as a shell layer, a near infrared fluorescent dye for photodynamic therapy is modified on the outer surface of the shell layer. The preparation method comprises the steps that a chloroauric acid solution and a cetyl trimethyl ammonium bromide solution are mixed, a sodium borohydride ice water mixed solution is added to obtain a nano gold seed solution A; a silver nitrate solution and a chloroauric acid solution are added to a binary surface active agent solution, a hydrochloric acid is added after reaction to obtain a solution B, then ascorbic acid and the solution A are added, and a gold nanorod is obtained after reaction; a tetraethoxysilane methanol solution and an aminopropyl trimethoxy silane methanol solution are added to a gold nanorod solution to obtain composite nanoparticles; 1-(3-dimethyl amino propyl)-3-ethyl carbodiimide hydrochloride is added to the near infrared fluorescent dye, then N-hydroxyl succinimide is added to obtain a solution C; the composite nanoparticle solution is mixed with the solution C to obtain a product.

Description

technical field [0001] The invention relates to a nanocomposite carrier, in particular to a gold nanorod composite carrier with photothermal / photodynamic therapy properties and a preparation method thereof. Background technique [0002] Malignant tumor (cancer) is the second largest disease that seriously threatens human health, with low cure rate and high mortality. The incidence of cancer in my country is currently in a period of rapid rise. According to the World Health Organization's global cancer report: in 2015, more than 2.8 million people died of cancer in my country. At present, conventional treatment methods such as radiotherapy, chemotherapy, and surgical treatment are mostly used clinically, but these methods have great limitations, such as drug resistance, high late metastasis rate, and poor prognosis (Omoti, A.E. & Omoti, C.E. Ocular toxicity of systemic anticancer chemotherapy. Pharm Pract (Granada) 4, 55-59 (2006)). Therefore, new tumor treatment methods su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00B82Y5/00A61K47/48A61P35/00
CPCA61K41/0052A61K41/0057A61K49/0065A61K49/0093B82Y5/00A61K2300/00
Inventor 任磊王秀敏陆敏丹亢宁
Owner XIAMEN UNIV
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