Targeted nanoparticle drug delivery system of accessory immune cell therapy and preparation method thereof

An immune cell therapy and nano-drug-loading technology, applied in the field of targeted nano-drug-loading systems, can solve the problems of high dosage of anti-tumor drugs for tumor cells, inability to identify all tumor cells, and large toxic and side effects of normal cells, and avoid space Steric hindrance effect, avoid toxic side effects, high affinity effect

Active Publication Date: 2016-09-21
徐妍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the shortcomings of the above-mentioned immune cell therapy that cannot fully recognize and kill tumor cells, as well as the shortcomings of existing anti-tumor drugs such as high dosage, short half-life in vivo, large toxic and side effects on normal cells, and lack of specificity for tumor cells, this The primary purpose of the invention is to provide an active targeting carbon quantum dot nano drug-loading system and its preparation method for assisting immune cell therapy to achieve efficient double killing of tumor cells

Method used

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  • Targeted nanoparticle drug delivery system of accessory immune cell therapy and preparation method thereof
  • Targeted nanoparticle drug delivery system of accessory immune cell therapy and preparation method thereof
  • Targeted nanoparticle drug delivery system of accessory immune cell therapy and preparation method thereof

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Embodiment 1

[0047] The targeted nano-drug loading system of the present invention uses nano-material carbon quantum dots CQDs as the carrier, polyethylene glycol PEG as the cross-linked product, transferrin Tf as the targeting molecule, and is formed by covalent coupling. The nano-carrier CQDs-PEG-Tf has a particle size of 224.5±90.2nm. structured as figure 1 shown.

[0048] Its preparation process is as follows:

[0049] (1) Preparation of carrier carbon quantum dots-polyethylene glycol (CQDs—PEG—OH)

[0050] Dissolve 0.2g of ascorbic acid in 2mL of ultrapure water, and stir it magnetically for 15min to form a transparent and uniform solution. The solvent filter is filtered, and washed and purified in a dialysis bag (molecular weight cut off: 8000) to obtain an aqueous solution of carbon quantum dots. The obtained aqueous solution of carbon quantum dots is evaporated in a rotary evaporator to remove most of the solvent, and then dried in a vacuum oven at 35-65° C. to obtain carbon qu...

Embodiment 2

[0064] The targeted nano-drug loading system of the present invention uses nano-material carbon quantum dots CQDs as the carrier, polyethylene glycol PEG as the cross-linked product, transferrin Tf as the targeting molecule, and is formed by covalent coupling. The nano-carrier CQDs-PEG-Tf has a particle size of 224.5±90.2nm. structured as figure 1 shown.

[0065] Its preparation process is as follows:

[0066] (1) Preparation of carrier carbon quantum dots-polyethylene glycol (CQDs—PEG—OH)

[0067] Dissolve 0.2g of ascorbic acid in 2mL of ultrapure water, stir it magnetically for 15min to form a transparent and uniform solution, add 4mL of PEG-200 and stir for 20min, use a microwave power of 300W, set the temperature at 85°C, and microwave for 140s. The solvent filter is filtered, and washed and purified in a dialysis bag (molecular weight cut off: 8000) to obtain an aqueous solution of carbon quantum dots. The obtained aqueous solution of carbon quantum dots is evaporated...

Embodiment 3

[0081] The targeted nano-drug loading system of the present invention uses nano-material carbon quantum dots CQDs as the carrier, polyethylene glycol PEG as the cross-linked product, transferrin Tf as the targeting molecule, and is formed by covalent coupling. The nano-carrier CQDs-PEG-Tf has a particle size of 224.5±90.2nm. structured as figure 1 shown.

[0082] Its preparation process is as follows:

[0083] (1) Preparation of carrier carbon quantum dots-polyethylene glycol (CQDs—PEG—OH)

[0084] Dissolve 0.2g of ascorbic acid in 2mL of ultrapure water, stir it magnetically for 15min to form a transparent and uniform solution, add 4mL of PEG-200 and stir for 20min, use a microwave power of 400W, set the temperature at 100°C, and microwave for 200s. The solvent filter is filtered, and washed and purified in a dialysis bag (molecular weight cut off: 8000) to obtain an aqueous solution of carbon quantum dots. The obtained aqueous solution of carbon quantum dots is evaporate...

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Abstract

The invention discloses a targeted nano carbon quantum dot carrier CQDs-PEG-Tf of an accessory immune cell therapy and a preparation method thereof and application of a carrier mediated antitumor drug an immune cells in the targeted tumor therapy. It is proved through in vitro experiments that the carrier can be combined with the tumor cell surface over-expression receptor specifically, and the good biological safety to immune cells is achieved by means of a carrier mediated antitumor drug under a certain concentration. The carrier mediated antitumor drug is combined with the immune cells to double target and kill tumor cells, the double anti-tumor effect is achieved, and toxic and side effects of the antitumor drug on normal cells can be reduced. The carrier is simple in preparation method and easy to carry out and has a high targeting property, nontoxicity, excellent biocompatibility and biodegradability. The carrier can be applicable to preparation of targeted anticancer drugs and the targeted therapy of accessory immune cells on various cancer diseases.

Description

technical field [0001] The invention relates to a targeted nano-drug loading system for assisting immune cell therapy, in particular to a carbon quantum dot nano-targeted carrier and a preparation method thereof as well as the preparation of targeted anti-tumor drugs and the combination of the targeted anti-tumor drugs with immune cells application in tumor targeted therapy. technical background [0002] At present, somatic cell immunotherapy has become one of the important means of adjuvant therapy after surgery, radiotherapy and chemotherapy for cancer patients. It has good effects on promoting the reconstruction of the patient's immune system, eliminating residual lesions and purifying bone marrow. Studies have shown that immune cells have dual functions of killing tumor cells and improving anti-tumor immunity. They can chemotaxis and recognize tumor cells, and have obvious killing effects on tumor cells, tumor stem cells and other tumor cells in the non-proliferative pha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/02A61K47/34A61K35/17A61P35/00
Inventor 徐妍陈蕾郭世磊
Owner 徐妍
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