Method for pretreating lignocellulose by using renewable ionic liquid aqueous solution

A technology of lignocellulose and ionic liquid, which is applied in the field of lignocellulose utilization and chemical industry application, can solve the problems of reducing the economic feasibility of the pretreatment process, stirring, filtering, centrifuging difficulties, reducing the yield of reducing sugar, etc., and achieves super Strong water resistance, lower system viscosity, lower energy consumption

Inactive Publication Date: 2013-04-17
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

[0004] However, the viscosity of ionic liquids is much higher than that of conventional molecular solvents, which makes stirring, filtering, centrifuging and other operations extremely difficult during the pretreatment process.
In addition, hemicellulose is a polymer sensitive to acid, alkali and heat, which is easily lost during pretreatment, reducing the yield of reducing sugar
At the same time, the use of pure ionic liquid as a pretreatment solvent requires drying of the ionic liquid and the lignocellulosic raw material to be pretreated, resulting in high energy consumption, which significantly reduces the economic feasibility of the pretreatment process, so it is urgent to develop highly resistant Ionic liquid pretreatment process subject to moisture
At present, the price of ionic liquids is still relatively high, and there is still a relatively high cost of using such solvents to pretreat lignocellulose

Method used

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  • Method for pretreating lignocellulose by using renewable ionic liquid aqueous solution
  • Method for pretreating lignocellulose by using renewable ionic liquid aqueous solution

Examples

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Effect test

Embodiment 1

[0027] 50wt%[choline][glycine] aqueous solution pretreatment of rice straw

[0028] a) Pretreatment: Accurately weigh 150mg of rice straw powder (150~350μm) and 3g of [choline][glycine] ionic liquid aqueous solution (the content of ionic liquid is 50wt%; Synthesized by the method described above), put them together in a 15mL Erlenmeyer flask, and stirred at 90°C for 12 hours under the protection of nitrogen; then cooled to room temperature, added 1 times the volume of the pretreatment solvent to dilute with deionized water, filtered, and then used 3 times the volume of deionized water to wash the filter residue 5 times, and the filter residue was dried to obtain the pretreated rice straw.

[0029] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U of Trichoderma reesei Cellulase (purchased from Sigma-Aldrich China Branch) was sealed and placed in a ...

Embodiment 2

[0031] 50wt%[choline][tryptophan] aqueous solution pretreatment of rice straw

[0032] a) Pretreatment: Accurately weigh 150 mg of rice straw powder (150–350 μm) and 3 g of [choline][tryptophan] ionic liquid aqueous solution (50 wt% ionic liquid content), put them together in a 15 mL Erlenmeyer flask, and Under protection, stir at 90°C for 12 hours; then cool to room temperature, add 2 times the volume of pretreatment solvent with deionized water to dilute, filter, then wash the filter residue with 3 times the volume of deionized water for 5 times, and the filter residue is dried That is, the pretreated rice straw is obtained.

[0033] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U of Trichoderma reesei Cellulase, after being sealed, is placed in a constant temperature oscillator at 200r / min and 50°C for reaction. After 12 hours, 200 μL was sam...

Embodiment 3

[0035] 50wt%[choline][phenylalanine] aqueous solution pretreatment of rice straw

[0036] a) Pretreatment: Accurately weigh 150mg of rice straw powder (150-350μm) and 3g of [choline][phenylalanine] ionic liquid aqueous solution (50wt% ionic liquid content), put them together in a 15mL Erlenmeyer flask, Under the protection of nitrogen, stir at 90°C for 12 hours; then cool to room temperature, add 1 times the volume of pretreatment solvent with deionized water to dilute, filter, then wash the filter residue with 3 times the volume of deionized water for 5 times, and dry the filter residue After that, the pretreated rice straw is obtained.

[0037] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U of Trichoderma reesei Cellulase, after being sealed, is placed in a constant temperature oscillator at 200r / min and 50°C for reaction. After 12 hours, 200...

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Abstract

The invention discloses a method for pretreating lignocellulose by using a renewable ionic liquid aqueous solution, including the steps of: (1) taking a choline and amino acid ionic liquid aqueous solution as a pretreatment solvent, mixing lignocellulose and the pretreatment solvent under the protection of nitrogen, stirring at 50-120 DEG C, cooling to room temperature, filtering, washing residue, and drying to obtain the pretreated lignocellulose; and (2) weighing the pretreated lignocelluloses, adding a citrate buffer, adding cellulase, reacting for 3-12 h with 150-250 r / min at 40-60 DEG C to obtain sugar liquid mainly including glucose and xylose. The pretreatment process not only can effectively enhance the efficiency of enzymatic hydrolysis of lignocelluloses, and improve the yield of fermentable reducing sugar, but also has the advantages of environmental protection, renewability, low viscosity, easy operation, low cost, and low power consumption.

Description

technical field [0001] The invention belongs to the field of lignocellulose utilization and chemical industry application, and specifically relates to a method for enhancing lignocellulose enzymatic hydrolysis by pretreatment of [choline][amino acid] ionic liquid aqueous solution. Background technique [0002] With the increasing scarcity of petroleum resources, rising fuel prices and the outbreak of the energy crisis in recent years, the development and utilization of renewable resources such as biomass have gradually attracted attention. Compared with fossil resources, biomass resources have many advantages: their annual output is huge, and the production of fuel and chemical raw materials using biomass as raw materials can also effectively reduce CO 2 emissions and environmental pollution. As the most widely distributed and abundant renewable biomass resource on the earth, lignocellulose has attracted much attention. Lignocellulose is mainly composed of lignin, cellulos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCY02P20/54
Inventor 李宁宗敏华侯雪丹
Owner SOUTH CHINA UNIV OF TECH
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