Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicine composition for treating tumors and application thereof

A technology of composition and medicine, applied in the field of pharmaceutical use

Active Publication Date: 2014-07-23
ZHEJIANG UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the combined use of acridine yellow and alkylating agent for the treatment of tumors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine composition for treating tumors and application thereof
  • Medicine composition for treating tumors and application thereof
  • Medicine composition for treating tumors and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 : In colon cancer HCT116, cervical cancer Hela and multiple myeloma RPMI-8226 cell lines, the effects of acriflavine and melphalan alone or in combination on cell proliferation.

[0025] Experimental materials: colon cancer HCT116, cervical cancer Hela and multiple myeloma RPMI-8226 cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences; acridine yellow, melphalan and sulforhodamine B were purchased from Sigma; water-soluble tetrazolium salt -WST-8 kit was purchased from Dojindo Dongren Chemical Technology Company.

[0026] experimental method:

[0027] (1) Cell treatment: Cells were treated with 0.5X10 4 The density of cells per well was seeded in 96-well plates, pretreated by adding acriflavine DMSO solution (final concentration 0.5 or 0.75 μM) for 8 hours, and then adding gradient concentrations of melphalan for 24 hours.

[0028] (2) Determination of cell proliferation: For adherent cells, discard the supernatant, add ...

Embodiment 2

[0030] Example 2 : Acridine yellow increases tumor cell apoptosis induced by melphalan.

[0031] Experimental materials: the source of cell lines and medicines are the same as in Example 1. Ethidium bromide (PI) was purchased from BD Company.

[0032] Instrument: FACSCalibur flow cytometer was purchased from BD Company.

[0033] Experimental method: 10 cells per well for adherent cells 6 The density of each was seeded in Corning 6-well cell culture plates, and the suspension cells were seeded at 10 6 The density of each mL of culture medium is inoculated in a 3 mL cell culture flask. After overnight, acriflavine was added for pretreatment for 8 hours, and then melphalan was added for co-cultivation. After 24 hours, the cells collected by centrifugation were washed 3 times with PBS, fixed with 75% alcohol at -20°C for 2 hours, centrifuged and washed 3 times, then digested with PBS solution containing RNase for 30 minutes, and stained with ethidium bromide And the percent...

Embodiment 3

[0035] Example 3 : Acridine yellow can increase the effect of melphalan on transplanted tumors in nude mice

[0036] Experimental materials: The source of the drug is the same as that in Example 1. The nude mice are from Shanghai Slack Company, all of them are female, and they are 2-3 weeks old.

[0037]Experimental method: nude mice were randomly divided into 4 experimental groups, namely the control group, acridine yellow group, and melphalan combined with acridine yellow melphalan combined group, 5 mice in each group, subcutaneously inoculated Hela cell line or HCT116 cell line, 10 7 The tumor volume was measured and recorded every day or every other day. When the tumor grows to 70mm 3 At the same time, the control group was given normal saline, the acriflavine group was given 10 mg / kg, the melphalan group was given 0.25 mg / kg every other day, and the combination group was given both drugs. The experimental period is about 2 weeks.

[0038] Experimental results: The tu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a medicine composition for treating tumors and an application thereof. The medicine composition is composed of acriflavine or pharmaceutically acceptable salts thereof, melphalan or pharmaceutically acceptable salts thereof, and a medicinal carrier, wherein the molar ratio of the acriflavine to the melphalan is 1:100 to 1:10, and the tumors comprise blood tumors and solid tumors. The forms of the medicine comprise single preparations prepared from acriflavine or melphalan respectively, or a compound preparation prepared from acriflavine and melphalan. The medicine composition provided by the invention has the effects of improving the curative effect on tumors, the toxicity of the medicine composition can be reduced, and the growth speeds of the tumors can be slowed down.

Description

technical field [0001] The invention belongs to the field of pharmaceutical application, and relates to a combined pharmaceutical composition for treating tumors, in particular to a pharmaceutical composition of acriflavine and melphalan for treating tumors, and pharmaceutical applications thereof. Background technique [0002] Tumor is a serious public health problem in the world. According to the 2012 China Cancer Annual Report, the current incidence of cancer in my country is 285.91 / 100,000. The number of new cancer cases in the country is estimated to be about 3.12 million every year, with an average of 6 people per minute every day. diagnosed as a malignant tumor. The lifetime probability of Chinese residents suffering from cancer is 22%. The national cancer mortality rate is 180.54 / 100,000. An estimated 2.7 million cancer deaths occur each year. The probability of death of Chinese residents from cancer is 13%. [0003] Alkylating agents are commonly used clinical ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/473A61P35/00A61K31/196
Inventor 杨波何俏军曹戟朱虹应美丹林冠宇
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com