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Intestinal probiotics and application thereof in treating tumors and replacing antibiotics

A probiotic preparation, tumor technology, applied in the biological field, can solve the problems of drug-resistant bacteria, antibiotic residues, endangering life and health, etc.

Active Publication Date: 2021-06-11
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extensive use of antibiotics will not only lead to the emergence of drug-resistant bacteria, but also cause a large amount of antibiotic residues in meat, eggs, and milk, seriously endangering people's lives and health
At present, many countries have banned the use of antibiotics as animal growth promoters, however, stopping the use of antibiotics in animal feed has brought great challenges to the development of livestock and poultry farming

Method used

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  • Intestinal probiotics and application thereof in treating tumors and replacing antibiotics
  • Intestinal probiotics and application thereof in treating tumors and replacing antibiotics
  • Intestinal probiotics and application thereof in treating tumors and replacing antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1, JY strain radiation mutagenesis, screening and the acquisition of excellent mutant strain JY300-8

[0080] 1, 12 C 6+ Mutation of JY strain by ion irradiation

[0081] Using the isolation and purification method of Lactobacillus, Lactobacillus JY was isolated and purified from corn stalks with MRS medium, and the JY strain was identified as Lactobacillus casei by 16S rDNA molecular identification (such as figure 1 shown).

[0082] In order to further improve its probiotic performance, the heavy ion beam provided by the TR4 biological irradiation terminal of Lanzhou Heavy Ion Accelerator National Laboratory (HIRFL) 12 C 6+ , to irradiate the JY bacterial suspension. The dose rate was 40Gy / min, the irradiation energy was 80MeV / u, and the irradiation doses were 0, 40, 80, 120, 160, 200, 300, and 400Gy respectively. Three repetitions were set for each dose. The survival rate curves under different doses were as follows: figure 2 shown by figure 2 It ...

Embodiment 2

[0104] Embodiment 2, isolation, purification and identification of Lactobacillus reuteri JMR-01

[0105] Using the method of separation and purification of Lactobacillus, assisted by JY300-8 12 C 6+ Isolation and purification of dominant Lactobacillus JMR-01 from the intestinal tract of BABLC breast cancer mice cured by ions, specifically: collecting JY300-8 auxiliary 12 C 6+ The feces of the cured breast cancer mouse model were aseptically diluted according to the gradient dilution method (the dilution gradient was 10 -1 ,10 -2 ,10 -3 ,10 -4 ), pick a suitable gradient (10 -4 ) on the MRS solid agar medium by streaking on the plate, cultured at 37°C for 36 hours, picked the single colony with the largest colony shape and smooth surface on the MRS agar plate, and continued to purify as above for 2-3 The second time, a Lactobacillus strain JMR-01 dominant in the fecal liquid was obtained.

[0106] Lactobacillus strain JMR-01 was molecularly identified according to the f...

Embodiment 3

[0123] Embodiment 3, bacterial strain suppresses the research of tumor cell proliferation

[0124] 1. In vitro anti-breast cancer cell research

[0125] In order to further verify the inhibitory effect of the dominant Lactobacillus-Lactobacillus reuteri JMR-01 isolated and purified from the intestinal microbiome cured of breast cancer on breast cancer cells, the following different probiotics were used to detect the inhibition of breast cancer Tubo cells by MTT assay Proliferation ability: Lactobacillus casei (TD, isolated from the laboratory, identified as Lactobacillus casei by 16S rRNA (such as Figure 10 Shown)), Lactobacillus rhamnosus (L. rhamnosus, Lactobacillus rhamnosus is isolated from probiotic freeze-dried powder of Jilin S&P Biotechnology Co., Ltd., product batch number: XZ-JDQ-1808A), implemented Lactobacillus casei JY300-8 obtained in Example 1 and Lactobacillus reuteri JMR-01 obtained in Example 2.

[0126] Specifically:

[0127] Breast cancer Tubo cells (Fe...

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Abstract

The invention discloses intestinal probiotics and application thereof in treating tumors and replacing antibiotics. The lactobacillus reuteri JMR-01 provided by the invention has the preservation number of CGMCC No. 21304. The invention also provides a complex microbial inoculant of the complex microbial inoculant and the following application of the complex microbial inoculant: 1) treatment and / or prevention of tumors; 2) inhibiting of tumor cell proliferation; 3) preventing or treating of diseases caused by pathogenic bacteria; 4) replacing of antibiotics; 5) inhibiting of proliferation of pathogenic bacteria; and 6) promoting or synergistical treating of tumors. The invention provides intestinal probiotics, a method for treating tumors based on the intestinal probiotics and improving the tumor treatment effect by assisting irradiation treatment, and a method for treating tumors by taking the intestinal probiotics as a raw material.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a new strategy for treating tumors based on intestinal probiotics and the application of alternative antibiotics in animal husbandry, in particular to an intestinal probiotic and its application in treating tumors and replacing antibiotics. Background technique [0002] Human gut microbes refer to a large number of microorganisms on the intestinal mucosa, including bacteria, archaea, fungi, protozoa, and viruses. In the long-term evolution process, the intestinal microbial flora and the host have become a symbiotic The ecological system is closely related to the body's disease, metabolism, immunity and so on. The intestinal flora is mainly composed of symbiotic bacteria (mainly Bifidobacterium and Lactobacillus), opportunistic pathogenic bacteria (mainly Enterococcus, Escherichia coli) and pathogenic bacteria (such as Salmonella, Clostridium perfringens, etc. )composition. Many studi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P35/00A61P31/04C12Q1/689C12R1/225C12R1/245
CPCC12N1/20A61K35/747A61P35/00A61P31/04C12Q1/689Y02A50/30
Inventor 王曙阳肖国青许富强王俊凯董妙音李强
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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