Preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof

A heat-sensitive liposome, tumor treatment technology, applied in the field of medicine to achieve the effect of improving the effect, enhancing the sensitivity, and improving the therapeutic effect

Active Publication Date: 2017-08-18
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although hyperthermia cannot replace surgery, radiotherapy or chemotherapy as an independent tumor treatment plan, it has obvious synergistic and complementary effects on chemotherapy, radiotherapy and surgery and other tumor treatment methods

Method used

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  • Preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof
  • Preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof
  • Preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Synthesis and characterization of hollow gold nanoparticles

[0042] 100ml ultrapure water, add 1ml 0.05M sodium citrate solution and 100μL 0.4M cobalt chloride solution, nitrogen protection; add 1mL 0.1M sodium borohydride solution, stir for 15min, add 200μL 1% chloroauric acid, stir for 30min with air , Centrifuge at 15000 rpm for 15 minutes, and re-disperse and precipitate with ultrapure water to obtain hollow gold nanoparticles; use transmission electron microscope and ultraviolet full-wavelength scanner to detect the product, the results are as follows figure 1 with figure 2 .

Embodiment 2

[0044] Comparison of Photothermal Conversion Ability of Several Gold Nanomaterials

[0045] The solid gold nanoparticles, gold nanostars and hollow gold nanoparticles were prepared according to relevant literature methods, and concentrated by centrifugation to adjust the gold content to be the same; respectively take 1mL from the above solution into a quartz dish, and use the 808nm laser at 3w / cm 2 Irradiate for 10 minutes, the infrared thermometer detects the temperature every 1 minute, and uses normal saline as a control. The results are as follows image 3 .

Embodiment 3

[0047]

[0048] Weigh DPPC and DSPE-PEG2000 according to the prescription, mix them evenly, add organic solvent to dissolve, place on a rotary evaporator to remove the organic solvent to form a film, add concentrated hollow gold nanoparticle solution for hydration, and pass a 0.2μm filter membrane at 60°C For more than ten times, heat-sensitive liposomes containing hollow gold nanoparticles were prepared; the prescribed amount of adriamycin powder was added, dissolved at 60°C and incubated for 4 hours with stirring, and then allowed to cool and dialyzed to remove unencapsulated adriamycin , A heat-sensitive liposome preparation containing doxorubicin and hollow gold nanoparticles is obtained.

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Abstract

The invention relates to preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof. The preparation is mainly prepared from dipalmitoyl phosphatidylcholine (DPPC), hollow gold nanoparticles and therapeutic agents. A preparation method of the preparation comprises the following steps: preparing thermosensitive liposome via a conventional method, coating the thermosensitive liposome with the hollow gold nanoparticles by using a film extrusion method, and then coating the thermosensitive liposome with the therapeutic agents. The preparation is capable of reducing toxic and side effects of systemic drug therapy and is also capable of achieving the triple integration therapy effect with combination of drug therapy, thermal therapy and radiation therapy; the tumor killing effect is improved.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and relates to a heat-sensitive liposome co-carrying hollow gold nanoparticles and a tumor therapeutic agent, a preparation method thereof, and an anti-tumor application of "triple integration". Background technique [0002] Thermosensitive liposomes (thermosensitive liposomes), also known as temperature-sensitive liposomes, are liposomes that can release drugs under warm conditions. At a heating temperature higher than the liposome phase transition temperature, the liposome membrane changes from a "colloid state" to a liquid crystal structure, and the permeability increases, and the drug it carries is quickly released at the heated part to produce thermal targeting. It effectively utilizes the dual advantages of liposomes and hyperthermia, further increases the targeting of liposomes, reduces the uptake of liposomes by the reticuloendothelial system, improves the therapeutic effect and reduces toxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K45/00A61K41/00A61K47/02A61P35/00A61N5/10
CPCA61K9/127A61K41/0052A61K45/00A61K47/02A61N5/1001A61N2005/1085A61N2005/1092A61K2300/00
Inventor 沈雁尤晨王茹
Owner CHINA PHARM UNIV
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