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The present application belongs to the field of medicine and biotechnology, and particularly relates to the use of matrine in the preparation of a medicine for preventing or treating radiation damage. The radiation damage includes skin damage, lung damage, intestinal damage, hematopoietic damage, reproductive function damage, and delaying aging caused by radiation. The radiation includes non-ionizing radiation and ionizing radiation. The medicine further comprises other medicines for preventing or treating radiation damage. In the screening of anti-radiation medicines based on various cell models, it is found that matrine has a repair-promoting effect on small intestinal mucosal epithelial cell damage caused by radiation; through a mouse radiation damage model, it is found that matrine can effectively protect the body, reduce the death caused by a lethal dose of Co-60 gamma rays, and delay aging caused by radiation. Matrine has a good clinical application prospect in preventing or treating radiation damage, and has no obvious toxic side effects.