Ternary complex nanodrug, preparation method and application thereof in preparation of light controlled release nano-delivery system

Abstract

The invention discloses a ternary complex nanodrug, a preparation method and application thereof in preparation of a light controlled release nano-delivery system. The ternary complex nanodrug comprises a thioketal bond crosslinked low molecular weight PEI-based polymer, a hyaluronic acid polymer attached with a photosensitizer, and a nucleic acid drug. The photosensitizer contained in the complexcan generate reactive oxygen species (ROS) under the control of exogenous red light, realize photochemical internalization (PCI) mediated lysosome escape and photopromoted polymer degradation, thus finally realizing the purposes of reducing the toxic and side effects of materials, improving the transfection efficiency, and enhancing the tumor gene therapeutic effect.

Description

technical field

[0001] The invention belongs to the fields of polymer material technology and pharmacy, and relates to a preparation method and application of a light-controllable release nanometer delivery system. Background technique

[0002] As a commercial gene carrier, polyethyleneimine (PEI) can effectively carry negatively charged nucleic acids due to its structure containing a large number of positively charged amino groups, thereby forming a stable complex and further delivering into cells . However, while high-molecular cationic polymers have a large number of positive charges, the cytotoxicity of their materials cannot be ignored, and the nucleic acid materials encapsulated in them are difficult to release, which ultimately leads to limited transfection effects. The development of degradable cationic polymers can solve the above contradictions, realize nucleic acid entrapment and intracellular release, and reduce the cytotoxicity of polymer materials themselves. ...

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