92results about How to "Strong tissue penetration" patented technology

The present invention relates to optimized aptamers and methods of using these aptamers.

The invention belongs to the fields of radiopharmaceuticals and nuclear medicine and provides an imaging drug <68>Ga-NOTA-IF7 targeting Anxa1 in tumor blood vessels and a preparation method thereof. The drug <68>Ga-NOTA-IF7 is used for differential diagnosis and periodization of tumors, accurate positioning of focuses and curative effect monitoring. According to the invention, the tumor imaging drug <68>Ga-NOTA-IF7 is prepared through leaching of <68>Ga and <68>Ga-NOTA-IF7 labeling. The preparation method has the advantages of simple preparation technology, convenient operation, small consumption of time, a high labeling rate and a stable marker and facilitates further application of the drug in clinical practice, scientific research and medicinal development. The invention also provides a novel positive electron tumor imaging agent. The novel positive electron tumor imaging agent has good targeting to tumors, so tumor imaging effects are improved, and a visual tool is provided for differential diagnosis and periodization of tumors, accurate positioning of focuses and curative effect monitoring. <68>Ga-NOTA-IF7 is a labeled compound belonging to polypeptide and has the advantages of small molecular weight, low immunogenicity, good tissue penetrability, high tumor tissue affinity and good application prospects.

The present invention relates to fluorescent cobalamins and uses of these compounds. More particularly, this invention relates to fluorescent cobalamins that comprise a fluorescent, phosphorescent, luminescent or light-producing compound covalently linked to cobalamin. These fluorescent cobalamins can be used to as diagnostic and prognostic markers (a) to distinguish cancer cells and tissues from healthy cells and tissues, including identifying lymph nodes containing cancer cells, and (b) to determine if an individual will respond positively to chemotherapy using cobalamin-therapeutic bioconjugates.

The invention provides a nano antibody. As for the nano antibody, (1) an amino acid sequence shown in SEQ ID NO:1 is achieved; or (2) compared with the (1), the at least 70% identity, the at least 75%identity, the at least 80% identity, the at least 85% identity, the at least 90% identity, the at least 95% identity, and the at least 99% identity are achieved. According to the embodiments, the nano antibody is an antibody specifically targeting an ion channel TRPV3, the native conformation TRPV3 can be combined, and the nano antibody has the high water solubility, high tolerance, high stability, high antigen bondability, low immunogenicity, high tissue penetrating power, and high expressivity.

The invention discloses an anti-human MSLN (mesothelin) monoclonal antibody, which is a specific high-affinity nano antibody developed by taking human MSLN as a target and based on a phage display technology. Compared with the prior art, the invention allows the anti-human MSLN monoclonal antibody to be successfully prepared, which is good in specificity, high in affinity and capable of binding tohuman MSLN expressed on the cell surface, and the anti-human MSLN monoclonal antibody is a potential drug for tumor immunotherapy.

The present invention relates to optimized aptamers and methods of using these aptamers.

The invention discloses a metal phthalocyanine and application thereof in the fields of photothermal materials and photothermal therapy. Irradiated by near-infrared laser, a solution containing the metal phthalocyanine disclosed by the invention has a remarkable photothermic effect, capable of implementing photothermal therapy, and the metal phthalocyanine can be used as a photothermal material orto prepare medicines with the effect of photothermal therapy, and is of great significance for the development of multifunctional phototherapeutic medicines.

The invention discloses a preparation method and fluorescence detection application of a phosphorescence iridium complex. The phosphorescence complex has a chemical formula [Ir(CHO-btiq)2(bpy)][PF6]. The invention provides the preparation method of the phosphorescence iridium complex, and the application of the phosphorescence iridium complex to the detection of cysteine and homocysteine. The emission spectrum of the phosphorescent iridium complex is in the near-infrared region; biological imaging of the phosphorescent iridium complex has the advantages of little damage small, strong penetrability and little background spontaneous fluorescence; and the phosphorescent iridium complex has good application prospect in small animal in vivo fluorescence imaging. The iridium complex provided by the invention can realize high selectivity detection of cysteine and homocysteine, and provides the possibility for the construction of a simple and high-selectivity fluorescence chemical sensor for determination of cysteine and homocysteine.

The present invention relates to optimized aptamers and methods of using these aptamers.

The invention discloses a polydopamine-coated Prussian blue and silver-loaded nano-composite material, and a preparation method and an application thereof. The composite material is formed by coatingPrussian blue with polydopamine and then growing nano-silver on the polydopamine in situ, and the particle size of the composite material is 100-150 nm. The preparation method comprises the followingsteps: reacting potassium ferricyanide with polyvinylpyrrolidone to obtain Prussian blue, adding dopamine into a Tris-HCl solution of Prussian blue to prepare polydopamine-coated Prussian blue, and mixing and stirring the obtained polydopamine-coated Prussian blue dispersion, ammonia water, a reducing agent and a silver nitrate solution to react in order to make nano-silver grows on the coating layer polydopamine in situ in order to obtain the product. The nano-composite material has a good antibacterial effect, is not prone to causing drug resistance of bacteria, has good stability and dispersity, the preparation method has the advantages is simple and mild, short in time consumption, low in energy consumption and easy to realize large-scale production, and the nano-composite material canbe used for preparing photo-thermal antibacterial drugs or chronic traumatic infection treatment drugs.

The present invention relates to a receptor molecule which binds to TNF comprising all or a functional portion of the extracellular domain (ECD) of two or more TNF-Rs linked via one or more polypeptide linkers. The receptor can further comprise a signal peptide of a secreted protein, such as the signal peptide of the extracellular domain of the TNF-R or the signal peptide of a cytokine. The invention also relates to isolated DNA encoding a receptor molecule which binds to TNF, comprising two or more sequences encoding all or a functional portion of the ECD of TNF-Rs linked via one or more sequences encoding a polypeptide linker. The invention further relates to a method of making a construct which expresses all or a functional portion of the ECD of two or more TNF-Rs linked via one or more polypeptide linkers and cells which express the construct. The invention also relates to a method of inhibiting the biological activity of TNF in a host comprising administering to the host an effective amount of a receptor molecule of the present invention. The invention further relates to receptor molecules which bind cytokines that bind to receptor molecules comprising more than one subunit.

The invention discloses a nano antibody for resisting toxoplasma gondii SAG1 as well as a coding gene and application thereof. A VHH chain amino acid sequence of the nano antibody is as shown by SEQ ID NO. 4. The nano antibody comprises two VHH chains. The nano antibody for resisting the toxoplasma gondii SAG1 is obtained by immunizing a camel by virtue of toxoplasma gondii SAG1 antigen, obtainingan anti-SAG1 nano antibody library, and selecting a nano antibody with good performance from the nano antibody library. The nano antibody has high solubility and conformation stability and high antigen affinity and excellent tissue penetration capability, and has high affinity with SAG1 antigen, the affinity constant KD value is 1.66nM, the toxoplasma gondii can be efficiently detected, and the nano antibody can be used for preparing toxoplasma gondii detection kit.

The invention discloses a virus-like hollow oxide loaded near-infrared two-b region excited rare earth nanocrystal and a preparation method and application thereof. According to the nanocrystal and the preparation method, an Er and Ho doped NaErF4:2%Ho@NaYF4 rare earth nanomaterial is synthesized through a hydrothermal method, then the nanomaterial is modified on a surface of a virus-like hollow manganese oxide poured into IR1064 through an amidation reaction, and a fluorescent composite probe which is good in biocompatibility and responds to a tumor microenvironment is obtained. According to the fluorescent composite probe, after the manganese oxide is degraded at a tumor, manganese ions are used for chemodynamic therapy and nuclear magnetic resonance imaging contrast agents of metastasis, released rare earth nanocrystals have excitation of 1530 nm and emitted lights of 650 nm and 1180 nm, the light of 1180 nm can be used for tumor imaging navigation surgical resection with no background interference and high resolution and tissue penetrating power in a near-infrared second region, and the light of 650 nm can used for near-infrared first region fluorescence imaging.

The invention discloses an anti-human MSLN monoclonal antibody and an application thereof, and is a specific and high-affinity single-chain antibody developed by taking human MSLN as a target based ona phage display technology. Compared with the prior art, the anti-human MSLN monoclonal antibody is successfully prepared, the antibody is good in specificity and high in affinity and can be combinedwith human MSLN expressed on the surface of a cell, and the anti-human MSLN monoclonal antibody is a potential drug for tumor treatment.

The invention discloses an anti-toxoplasma gondii TRX (Thioredoxin) nano-antibody, an encoding gene and the application thereof. VHH chain amino acid sequence of the antibody is as shown in SEQ ID No.4. The nano-antibody comprises two VHH chains. The anti-toxoplasma gondii TRX nano-antibody is obtained by immunizing a camel by a toxoplasma gondii TRX antigen to obtain an anti-TRX nano-antibody library, and then screening to obtain the anti-toxoplasma gondii TRX nano-antibody with good performance from the nano-antibody library. The anti-toxoplasma gondii TRX nano-antibody has high water solubility and conformational stability, strong antigen affinity, excellent tissue penetrating capacity, can detect toxoplasma gondii highly effectively, and can be used for preparing a toxoplasma gondii detection kit.

The invention discloses a nano-antibody of carbofuran pesticide and a preparation method and application of the nano-antibody. An amino acid sequence of the nano-antibody is shown as SEQ ID NO.1, genes of the nano-antibody are encoded, and a nucleotide sequence of nano-antibody is shown as SEQ ID NO.2. The nano-antibody is against the carbofuran pesticide and can be used for detecting the carbofuran pesticide, the detection result is accurate, the effect is good, the stability is good, and the detection effect is good under conditions of high temperature and an organic solvent. The nano-antibody can be widely used for the detection of carbofuran pesticide residues in agricultural products and can be taken as a precursor, modifying can be carried out by random or site-specific mutagenesis techniques to obtain mutants with better properties (affinity, specificity, stability and the like), the nano-antibody can be used for developing and further used in fields of food, medicine, agriculture and the like, and the application and promotion value is great.

The invention discloses a compound I. The compound I has a structure shown in a formula I (the formula I is shown in the description), wherein R1 is an acyl group, phenyl group or alkyl oxycarbonyl group containing 2-5 carbon atoms; R2 is a halogen atom or hydrogen atom; R3 is an alkyl group containing 1-18 carbon atoms. The invention also discloses a synthesizing method of the compound I, a fluorescent probe containing the compound I and application. The fluorescent probe provided by the invention has the advantages that the selectivity is excellent, the reaction is rapid, and fibroblast activating protein alpha can be simply, rapidly and visually detected.

The invention discloses a water-soluble anticancer photosensitizer and preparation and application thereof. Firstly, mother nucleus of monocarboxyl substituted fluoroboron dipyrrol is prepared from the raw materials of carboxybenzaldehyde, 2,4-dimethyl pyrrole and the like; secondly, two heavy atomic iodines are introduced to the 2th and 6th sites to increase the oxygen quantum yield in triplet and singlet states; thirdly, the compound is further subjected to a condensation reaction with carboxybenzaldehyde, the conjugated system is expanded, the compound is absorbed and moved to a red region,and two carboxyl groups are simultaneously introduced to obtain a water-soluble fluoroboron dipyrrol anti-cancer photosensitizer. The synthetic method of this compound is simple, the raw material iseasy to obtain, the cost is low, the side reactions are few, the yield is high, the purification is easy, and the compound is conducive to industrial production.

The invention discloses a paraquat hapten. The paraquat hapten has a structure represented by formula (I) shown in the description. A group with a high electron cloud density is introduced into the paraquat hapten, and after a carrier protein is coupled, the paraquat hapten can be fully exposed to obtain a paraquat complete antigen with strong immunogenicity. The invention discloses a paraquat specific antibody based on the paraquat complete antigen. The paraquat specific antibody has the advantages of high titer, strong specificity and high sensitivity. The paraquat detection test paper comprises a chromatographic membrane and a colloidal gold module, and the colloidal gold module comprises a colloidal gold labeled paraquat specific antibody; and a detection line and a quality control line are sequentially arranged on the chromatographic membrane along the liquid chromatography direction, the detection line is coated with the paraquat complete antigen, and the quality control line iscoated with a binding antibody of the paraquat specific antibody. The paraquat detection test paper can realize sensitive and rapid detection of paraquat residues, is simple in sample pretreatment, and is suitable for large-scale detection.

The invention provides a near-infrared quantum dot probe for the brain glioma tracing. The probe uses a carboxylated poloxamer, and a cyclic glycyl-arginyl-glycyl-aspartyl-asparaginyl-proline short peptide grafted poloxamer as materials, near-infrared quantum dots and a cysteine-alanine-glutamine-lysine tetrapeptide are encapsulated, nanomicelles are formed by the self-assembly in an aqueous solution, and a solution of the near-infrared quantum dot probe that specifically binds to integrin alpha v beta 3 overexpressed by glioma cells is formed. The near-infrared quantum dot probe has the characteristics of uniform particle size, high stability, good tumor targeting, and strong tissue penetration. The brain glioma tracing with high efficiency, targeting and specificity can be realized by combining the ultrasound-targeted micro-bubble blasting technology to reversibly open the blood-brain barrier.