Near-infrared quantum dot probe for brain glioma tracing

A glioma, near-infrared technology, applied in preparations for in vivo experiments, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problem that quantum dots cannot be directly applied, quantum dots are easy to aggregate, Instability and other problems, to achieve the effect of uniform particle size, good tumor targeting and high safety

Active Publication Date: 2019-08-23
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, quantum dots cannot be directly applied to the body. The main reason is that quantum dots are easy to aggregate in the blood circulation, unstable and have certain toxic side effects.
However, there are no reports of related technologies

Method used

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  • Near-infrared quantum dot probe for brain glioma tracing
  • Near-infrared quantum dot probe for brain glioma tracing
  • Near-infrared quantum dot probe for brain glioma tracing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of near-infrared quantum dot nanoprobes

[0028] (1) Synthesis of carboxylated poloxamer: dissolve poloxamer 188, succinic anhydride, 4-dimethylaminopyridine, and triethylamine in anhydrous 1,4-dioxane and stir at room temperature Overnight, then dialyzed at room temperature for 3 days using a dialysis bag (MWCO=3500D), and finally freeze-dried for 2 days under vacuum conditions by a freeze dryer to obtain a lyophilized product, which is a carboxylated poloxamer.

[0029] (2) Morpholineethanesulfonic acid monohydrate (MES) was dissolved in distilled water and stirred for 10 minutes, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride ( EDC) and N-hydroxysuccinimide (NHS) and stir until the solute is completely dissolved in distilled water, then add carboxylated poloxamer (Poloxamer-COOH) and cyclic glycyl-arginyl-glycine Acyl-aspartyl-asparaginyl-proline short peptide (c(RGDyk)) was fully stirred at room temperature for 24 hours, dialy...

Embodiment 2

[0035] Example 2 Performance characterization and application effect of near-infrared quantum dot nanoprobe (QDs-c(RGDyk) NP)

[0036](1) Characterization of near-infrared quantum dot nanoprobes: for 7 consecutive days, the particle size distribution of near-infrared quantum dot nanoprobes was measured using a LitesizerTM 500 particle size analyzer, and NQDs-c (RGDyk ) NPs were subjected to particle size measurements and morphology observations. The particle size distribution (narrow particle size distribution is better), microscopic shape (round surface and no aggregation is better), and stability (longer microscopic shape retention time is better) are used as indicators for comprehensive evaluation, with a full score of 10.

[0037] (2) In vitro cytotoxicity experiment of near-infrared quantum dot nanoprobes: CCK-8 kit was used to evaluate the in vitro cell activity of PCl2, C6 and HUVEC cells exposed to near-infrared quantum dot nanoprobes. C6 cells were seeded into 96-wel...

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Abstract

The invention provides a near-infrared quantum dot probe for the brain glioma tracing. The probe uses a carboxylated poloxamer, and a cyclic glycyl-arginyl-glycyl-aspartyl-asparaginyl-proline short peptide grafted poloxamer as materials, near-infrared quantum dots and a cysteine-alanine-glutamine-lysine tetrapeptide are encapsulated, nanomicelles are formed by the self-assembly in an aqueous solution, and a solution of the near-infrared quantum dot probe that specifically binds to integrin alpha v beta 3 overexpressed by glioma cells is formed. The near-infrared quantum dot probe has the characteristics of uniform particle size, high stability, good tumor targeting, and strong tissue penetration. The brain glioma tracing with high efficiency, targeting and specificity can be realized by combining the ultrasound-targeted micro-bubble blasting technology to reversibly open the blood-brain barrier.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of nano-probe tracing, in particular to a near-infrared probe for tracing glioma in combination with ultrasound-targeted microbubble blasting technology and a preparation method thereof. [0003] 【Background technique】 [0004] Glioblastoma is the most common primary malignant tumor of the central nervous system, accounting for 80% of malignant brain tumors, and is characterized by easy metastasis, unclear boundaries, high recurrence rate, high mortality rate, and poor prognosis. The median survival rate of patients is 15-23 months, and the 5-year survival rate is less than 6%. The standard treatment for glioblastoma is surgical resection combined with radiotherapy and adjuvant temozolomide. Nevertheless, the boundary between glioblastoma cells and normal brain tissue cells is blurred due to the characteristic of aggressive tumor growth and peritumoral brain tissue edema. Therefore, incomplete surgical resec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06B82Y20/00B82Y30/00B82Y40/00A61K49/00
CPCC09K11/025C09K11/06B82Y20/00B82Y30/00B82Y40/00A61K49/0067
Inventor 鲁翠涛陈斌赵应征吴其隆诸葛德力童梦琪
Owner WENZHOU MEDICAL UNIV
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