Near-infrared quantum dot probe for brain glioma tracing
A glioma, near-infrared technology, applied in preparations for in vivo experiments, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problem that quantum dots cannot be directly applied, quantum dots are easy to aggregate, Instability and other problems, to achieve the effect of uniform particle size, good tumor targeting and high safety
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Embodiment 1
[0027] Example 1 Preparation of near-infrared quantum dot nanoprobes
[0028] (1) Synthesis of carboxylated poloxamer: dissolve poloxamer 188, succinic anhydride, 4-dimethylaminopyridine, and triethylamine in anhydrous 1,4-dioxane and stir at room temperature Overnight, then dialyzed at room temperature for 3 days using a dialysis bag (MWCO=3500D), and finally freeze-dried for 2 days under vacuum conditions by a freeze dryer to obtain a lyophilized product, which is a carboxylated poloxamer.
[0029] (2) Morpholineethanesulfonic acid monohydrate (MES) was dissolved in distilled water and stirred for 10 minutes, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride ( EDC) and N-hydroxysuccinimide (NHS) and stir until the solute is completely dissolved in distilled water, then add carboxylated poloxamer (Poloxamer-COOH) and cyclic glycyl-arginyl-glycine Acyl-aspartyl-asparaginyl-proline short peptide (c(RGDyk)) was fully stirred at room temperature for 24 hours, dialy...
Embodiment 2
[0035] Example 2 Performance characterization and application effect of near-infrared quantum dot nanoprobe (QDs-c(RGDyk) NP)
[0036](1) Characterization of near-infrared quantum dot nanoprobes: for 7 consecutive days, the particle size distribution of near-infrared quantum dot nanoprobes was measured using a LitesizerTM 500 particle size analyzer, and NQDs-c (RGDyk ) NPs were subjected to particle size measurements and morphology observations. The particle size distribution (narrow particle size distribution is better), microscopic shape (round surface and no aggregation is better), and stability (longer microscopic shape retention time is better) are used as indicators for comprehensive evaluation, with a full score of 10.
[0037] (2) In vitro cytotoxicity experiment of near-infrared quantum dot nanoprobes: CCK-8 kit was used to evaluate the in vitro cell activity of PCl2, C6 and HUVEC cells exposed to near-infrared quantum dot nanoprobes. C6 cells were seeded into 96-wel...
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