Nanoscale lactoferrin chitosan particle preparation method

A technology of lactoferrin and chitosan, which is applied in the direction of peptide/protein components, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of low encapsulation efficiency and drug loading rate, particle size Large and other problems, to achieve the effect of simple operation, strong biological activity, and enhanced binding degree

Inactive Publication Date: 2016-12-07
HEILONGJIANG HEIKE TECH CO LTD
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Problems solved by technology

[0004] The present invention aims to solve the problems that the existing lactoferrin-chitosan particles have larger particle diameters, are mic...

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  • Nanoscale lactoferrin chitosan particle preparation method

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specific Embodiment approach 1

[0026] Specific embodiment one: the preparation method of nanoscale lactoferrin chitosan particle of the present embodiment, carry out according to the following steps:

[0027] 1. Reagent handling:

[0028] Dissolving chitosan in glacial acetic acid, adjusting the pH to 4-5, and preparing a chitosan solution with a concentration of 0.6-0.8 mg / mL;

[0029] Add 9-11 mg of lysozyme to 1 ml of sterile water or 1 ml of 10 mmol / L Tris·Cl (pH8.0) solution to obtain a lysozyme mixture;

[0030] Dissolve lactoferrin in 0.1mol / L sodium chloride solution, oscillate and mix to obtain a lactoferrin solution with a concentration of 9-11mg / mL;

[0031] 2. Chitosan modification:

[0032] Add an appropriate amount of lysozyme mixture to the chitosan solution, shake at 38-42°C at 75-85 rpm for 3-4 hours, degrade the long-chain chitosan into chitooligosaccharides, and then heat and boil for 3-5 minutes to make Lysozyme is inactivated to obtain a modified chitosan solution;

[0033] 3. Cross...

specific Embodiment approach 2

[0037] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, a chitosan solution with a concentration of 0.7 mg / mL is prepared. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0038] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 1, a lactoferrin solution with a concentration of 10 mg / mL is prepared. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a lactoferrin chitosan particle preparation method, in particular to a nanoscale lactoferrin chitosan particle preparation method and aims to solve problems of large particle size and low encapsulation efficiency and drug loading ratio of existing lactoferrin chitosan particles. The method includes: firstly, preparing chitosan solution, lysozyme mixed liquid and lactoferrin solution; secondly, adding the lysozyme mixed liquid into the chitosan solution, shaking, and boiling to obtain modified chitosan solution; thirdly, mixing the lactoferrin solution with the modified chitosan solution, stirring, adding sodium tripolyphosphate and stirring; fourthly, centrifuging to obtain precipitates which are the lactoferrin chitosan particles. Chitosan oligosaccharide prepared according to the method is conducive for bodies to absorb and high in bioactivity, protonic amino groups are easier to expose and crosslink with phosphate groups as compared with long-chain chitosan molecules, and accordingly binding degree of a drug loading system is raised. In addition, the encapsulation efficiency reaches 30%, the drug loading ratio reaches 26%, and the lactoferrin chitosan particles are used for preparation of lactoferrin medicines for oral administration.

Description

technical field [0001] The invention relates to a preparation method of lactoferrin chitosan particles. Background technique [0002] Lactoferrin is a protein-based therapeutic drug with a wide range of biological functions. Although it is convenient to administer lactoferrin directly through oral administration, it is necessary to overcome a large number of obstacles for protein-based drugs to enter the blood circulation from the digestive system. Carriers can protect environment-sensitive protein drugs from degradation while maintaining their own slow-release characteristics, so they are an ideal combination of drug-loaded particles. [0003] At present, the microparticles of lactoferrin-chitosan are prepared, and the particle size is on the order of microns. At the same time, the preparation method is such as the emulsification cross-linking method, and the required cross-linking agent such as glutaraldehyde has certain toxicity, which is not suitable for direct action on...

Claims

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Application Information

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IPC IPC(8): A61K38/40A61K9/51A61K47/36C08L5/08C08K3/32C08J3/24
CPCA61K38/40A61K9/5161C08J3/24C08J2305/08C08K3/32C08K2003/324C08L5/08
Inventor 孙尧王雷曹涤非黄国庆李瑶薛佳莹吴琼赵金海王东凯
Owner HEILONGJIANG HEIKE TECH CO LTD
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