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Preparation method of cordycepin controlled-release magnetic nanospheres

A magnetic nanometer and cordycepin technology, which can be used in medical preparations containing active ingredients, organic active ingredients, urinary system diseases, etc., can solve the problems of unfavorable clinical research and application of cordycepin, high price, etc. Effects of Metabolic Time and Concentration

Inactive Publication Date: 2014-04-16
WUYI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2’-dCF is produced by SuperGen in the United States, and its global sales are currently limited to North America and Europe. The price is expensive, which is not conducive to the large-scale clinical research and application of cordycepin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: 0.5g average particle diameter is the Fe of 20nm 3 o 4 Dissolve magnetic nanoparticles and 1g of sodium alginate in 50ml of distilled water at a temperature of 50°C, stir ultrasonically and at high speed for 30 minutes to form a magnetic fluid mixture, and add the mixed magnetic fluid mixture dropwise to the In 100ml of cyclohexane oil phase with 10g Span60 dissolved, keep the temperature at 50°C to form a microemulsion system, then add 50ml of Cordycepin with a concentration of 200mg / l, calcium chloride mass ratio concentration of 6% and chitosan mass ratio of 1 % of 5% acetic acid dilute solution was added dropwise in the microemulsion, after reacting for 30min, washed with acetone distilled water, magnetically separated, and filtered to obtain magnetic calcium alginate-chitosan nanospheres, and then the magnetic nanospheres Pour into 50ml of quercetin alkaline aqueous solution with a concentration of 100mg / l as an adenosine deaminase inhibitor, filter ...

Embodiment 2

[0019] Embodiment 2: 0.5g average particle diameter is the Fe of 20nm 3 o 4 Dissolve magnetic nanoparticles and 1.2g of sodium alginate in 50ml of distilled water at a temperature of 50°C, stir ultrasonically and at high speed for 30 minutes to form a magnetic fluid mixture, and add the mixed magnetic fluid mixture dropwise under ultrasonic and high-speed stirring Add 100ml of n-hexane oil phase with 6g Span80 dissolved therein, keep the temperature at 50°C to form a microemulsion system, and then mix 50ml of Cordycepin with a concentration of 200mg / l, calcium chloride mass ratio concentration of 10% and chitosan mass ratio of 1 % of 4% acetic acid dilute solution was added dropwise in the microemulsion, after reacting for 30min, washed with acetone distilled water, magnetically separated, and filtered to obtain magnetic calcium alginate-chitosan nanospheres, and then the magnetic nanospheres Pour into 50ml of genistein alkaline aqueous solution with a concentration of 100mg / ...

Embodiment 3

[0020] Embodiment 3: 0.5g average particle diameter is the Fe of 20nm 3 o 4 Dissolve magnetic nanoparticles and 1g of sodium alginate in 50ml of distilled water at a temperature of 50°C, stir ultrasonically and at high speed for 30 minutes to form a magnetic fluid mixture, and add the mixed magnetic fluid mixture dropwise to the In 100ml of n-heptane oil phase dissolved with 15g of dioctyl sodium succinate, keep the temperature at 50°C to form a microemulsion system, and then add 50ml of cordycepin with a concentration of 200mg / l and calcium chloride with a mass ratio of 8 % and chitosan mass ratio of 1% 4% acetic acid dilute solution was added dropwise to the microemulsion, after 30min of reaction, washed with acetone distilled water, magnetic separation, and filtered to obtain magnetic calcium alginate-chitosan nanospheres , and then pour the magnetic nanospheres into 50ml of quercetin alkaline aqueous solution with a concentration of 80mg / l as an adenosine deaminase inhibi...

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Abstract

The invention discloses a preparation method of cordycepin controlled-release magnetic nanospheres, and belongs to the field of medicine-carrying controlled-release magnetic-targeting nanospheres. The cordycepin controlled-release magnetic nanospheres can delay the metabolic time of cordycepin in a body and has a magnetic targeting function. The preparation process comprises the following steps: dissolving Fe3O4 magnetic nanoparticles and sodium alginate in distilled water, dropwise adding a magnetic fluid mixed solution formed by mixing under ultrasonic and high-speed stirring conditions into an oil phase to form a microemulsion system, dropwise adding a chitosan, cordycepin and calcium chloride mixed solution with certain concentration into a microemulsion, reacting for a certain time, performing washing, magnetic separation and filtering to obtain magnetic calcium alginate-chitosan nanospheres, pouring the nanospheres into a water solution of quercetin or genistein, vibrating for absorption equilibrium, filtering, pouring into a chitosan solution, finishing the reaction, and finally performing washing, filtering and freeze-drying to obtain the cordycepin controlled-release magnetic nanospheres.

Description

technical field [0001] The invention discloses a method for preparing cordycepin slow-release magnetic nanometer microspheres, which belongs to the field of drug-loaded slow-release magnetic targeting nanometer microspheres. In particular, it relates to a method of coating Fe with sodium alginate 3 o 4 A preparation method of magnetic nanoparticles, which adsorb cordycepin and chitosan, adsorb quercetin, and finally coat and protect cordycepin slow-release magnetic targeting nano-microspheres with chitosan. Background technique [0002] Cordycepin cordycepin (3'-deoxyadenosine) has a variety of biological activities, and has effects on DNA and RNA synthesis, hnRNA and mRNA post-transcriptional modification, adenylyl cyclase activation, and specific protein synthesis at the molecular level. Inhibitory effect; at the cellular level, it can promote cell differentiation and enhance the chemotaxis of macrophage cell lines; at the overall level, it has antifungal, antimalarial, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/7076A61K47/02A61K47/36A61P13/12A61P35/00A61P35/02A61K31/352
Inventor 巫莹柱贾永堂
Owner WUYI UNIV
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