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Amino hyperbranched chlorogenic acid molecularly-imprinted magnetic nanosphere as well as preparation method and application thereof

A magnetic nanosphere and molecular imprinting technology, applied in chemical instruments and methods, material separation, analysis of materials, etc., to achieve good water solubility, good water solubility, and good dispersibility

Active Publication Date: 2017-09-29
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no relevant report on the preparation of chlorogenic acid molecularly imprinted polymers that can rapidly and selectively separate and enrich chlorogenic acid in complex matrices by using magnetic nanospheres and hyperbranched polymerization.

Method used

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  • Amino hyperbranched chlorogenic acid molecularly-imprinted magnetic nanosphere as well as preparation method and application thereof
  • Amino hyperbranched chlorogenic acid molecularly-imprinted magnetic nanosphere as well as preparation method and application thereof
  • Amino hyperbranched chlorogenic acid molecularly-imprinted magnetic nanosphere as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Step 1. Put 0.98g of ferric chloride, 3.46g of sodium acetate, 6.03g of hexamethylenediamine and 30mL of ethylene glycol in a reaction kettle, and react at 200°C for 9 hours. After the reaction, wash the reaction product with ultrapure water until neutral , vacuum-dried at 45° C. and 0.06 MPa for 4 hours to obtain amino magnetic nanospheres. Such as figure 1 As shown, the particle size of the prepared amino magnetic nanospheres is about 20nm.

[0034] Step 2: Take 100 mg of the amino magnetic nanospheres prepared in Step 1, then put 30 mL of absolute ethanol and 5 mL of methyl acrylate into a three-necked flask, react at room temperature for 5 hours, then add 20 mL of ethylenediamine-ethanol solution, 50 °C The reaction was stirred under low temperature for 3 hours. After the reaction, the reaction product was washed with ultrapure water to neutrality, and vacuum-dried at 45°C and 0.06MPa for 4 hours to obtain amino hyperbranched modified magnetic nanospheres; wherein ...

Embodiment 2

[0047] Step 1. Put 1.05g of ferric chloride, 3.98g of sodium acetate, 5.14g of hexamethylenediamine and 25mL of ethylene glycol in a reaction kettle, and react at 180°C for 8 hours. After the reaction, wash the reaction product with ultrapure water until neutral , vacuum-dried at 40°C and 0.07MPa for 5h to obtain amino magnetic nanospheres.

[0048] Step 2: Take 200 mg of the amino magnetic nanospheres prepared in Step 1, and then put 25 mL of absolute ethanol and 15 mL of methyl acrylate into a three-necked flask, and react at room temperature for 5 h. Then add 18mL of ethylenediamine-ethanol solution, stir and react at 60°C for 4h, after the reaction, wash the reaction product with ultrapure water until it is neutral, and dry it in vacuum at 40°C and 0.07MPa for 5h to obtain amino hyperbranched modified Magnetic nanospheres; wherein the ethylenediamine-ethanol solution is prepared by mixing ethylenediamine and absolute ethanol with a volume ratio of 1:1.5;

[0049] Step 3: ...

Embodiment 3

[0058] Step 1. Put 1.15g of ferric chloride, 4.06g of sodium acetate, 5.29g of hexamethylenediamine and 28mL of ethylene glycol in a reaction kettle, and react at 190°C for 8 hours. After the reaction, wash the reaction product with ultrapure water until neutral , vacuum-dried at 48°C and 0.06MPa for 6h to obtain amino magnetic nanospheres.

[0059] Step 2: Take 250 mg of the amino magnetic nanospheres prepared in Step 1, put 28 mL of absolute ethanol and 18 mL of methyl acrylate together in a three-necked flask, and react at room temperature for 6.5 h. Then add 22mL of ethylenediamine-ethanol solution, stir and react at 48°C for 5.6h, after the reaction, wash the reaction product with ultrapure water until it is neutral, and dry it in vacuum at 48°C and 0.06MPa for 6h to obtain the amino hyperbranched modification magnetic nanospheres. Wherein the ethylenediamine-ethanol solution is prepared by mixing ethylenediamine and absolute ethanol with a volume ratio of 1:2;

[0060]...

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Abstract

The invention discloses an amino hyperbranched chlorogenic acid molecularly-imprinted magnetic nanosphere as well as a preparation method and application thereof. The preparation method comprises the following steps: first synthesizing an amino magnetic nanosphere; then adding the amino magnetic nanosphere and methyl acrylate into ethanol, stirring at room temperature, adding ethanediamine, heating, and stirring, so as to obtain an amino hyperbranched modified magnetic nanosphere; adding the amino hyperbranched modified magnetic nanosphere and chlorogenic acid into ethanol, and adding dopamine hydrochloride so as to generate polymerization reaction; and separating out solid polymer generated in the reaction by virtue of an applied magnetic field, and carrying out elution and drying, so as to obtain a target product. By carrying out amino hyperbranching on a carrier, namely magnetic nanosphere, the number of amino active groups on the surface of the magnetic nanosphere is increased, and dopamine is taken as a functional monomer and a cross-linking agent, so that the preparation method is simple and mild in conditions, the prepared target product is uniform in particle size, strong in stability and good in water solubility, and high selective removal and enrichment of chlorogenic acid in a complex matrix can be rapidly realized.

Description

technical field [0001] The invention relates to the technical fields of molecular recognition, magnetic separation, nanomaterials, food analysis, etc., and specifically relates to an amino hyperbranched chlorogenic acid molecularly imprinted magnetic nanosphere and a preparation method and application thereof. Background technique [0002] Chlorogenic acid (CGA) is a polyphenolic compound found in a variety of dietary plants and medicinal plants, which has antioxidant activity, anti-inflammatory effect, antibacterial, hypoglycemic, hypolipidemic, antitumor and Antihypertensive effect. [0003] CGA is easily soluble in water, and usually some compounds with similar functional groups coexist with it, such as caffeic acid, gallic acid and so on. Therefore, selective extraction of CGA from complex matrices is first required before quantification of CGA. [0004] At present, the commonly used methods for detecting chlorogenic acid in complex matrices mainly include chromatograp...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30B01D15/08G01N30/02G01N30/14
CPCB01D15/08G01N30/02G01N30/14B01J20/268B01J2220/48
Inventor 高瑞霞赵雅韵张军杰唐玉海
Owner XI AN JIAOTONG UNIV
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