Composite nano-drug for integrated tumor diagnosis and treatment and preparation method thereof

A technology of nano-drugs and chemotherapeutic drugs, applied in the direction of anti-tumor drugs, preparations for in vivo experiments, drug combinations, etc., can solve the problems of delayed treatment time and delivery difficulties for tumor patients

Inactive Publication Date: 2016-09-28
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is relatively difficult to deliver negatively charged biomolecules such as siRNA into tumor cells. Therefore, designing, modifying, and developing a new type of multifunctional drug carrier that can effectively realize the function of RNA silencing is an important aspect in the research of siRNA targeted gene silencing. biggest challenge
[0010] At present, the separation of clinical tumor diagnosis and treatment, due to its inherent limitations, cannot make accurate judgments and effective treatment of tumors in a timely manner, often delaying the best treatment time for tumor patients. Therefore, it is necessary to develop a multi-function integrated Diagnosis and treatment drugs, which combine diagnosis and treatment organically and synchronously, so as to achieve the purpose of diagnosis and treatment at the same time

Method used

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  • Composite nano-drug for integrated tumor diagnosis and treatment and preparation method thereof
  • Composite nano-drug for integrated tumor diagnosis and treatment and preparation method thereof
  • Composite nano-drug for integrated tumor diagnosis and treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for preparing a nanocarrier for co-transporting drugs and genes, the specific steps are as follows:

[0051] A1: Prepare Fe with a concentration of 5mg / ml 3 o 4 -Au solution, add cetyltrimethylammonium bromide (CTAB) as a template, stir for 5 minutes to mix evenly, then add deionized water and ammonia water, stir for 2 hours under 40 ° C water bath conditions, add to the Add tetraethyl orthosilicate (TEOS) to the solution as a silicon source, adjust the pH to weakly alkaline with ethyl acetate (EA), and continue to stir and react in a water bath at 40°C for 6 hours. After the reaction is completed, cool to room temperature, The reaction product was centrifuged, and the precipitate was washed repeatedly with absolute ethanol for 6 times, and the product was dissolved in absolute ethanol to prepare the Fe containing template. 3 o 4 -Au@mSiO 2 a solution of nanoparticles;

[0052] A2: Ammonium nitrate (NH 4 NO 3 ) dissolved in absolute ethanol and then heat...

Embodiment 2

[0058] A method for preparing a composite nanomedicine for the integration of tumor diagnosis and treatment, the specific steps are as follows:

[0059] Step A: Disperse the nanocarrier prepared in Example 1 in ultrapure water to prepare an aqueous nanocarrier solution with a concentration of 1 mg / ml. The nanocarrier uses magnetic nanospheres with noble metal nanoparticles arranged on the surface as cores, and uses amino groups modified on the surface The mesoporous silica is used as the shell, and the film layers of negatively charged polyelectrolyte and positively charged polyelectrolyte are sequentially formed on the surface of the core-shell structure through layer-by-layer self-assembly technology until the target number of layers is obtained; the polyelectrolyte The electrolyte layer is a polymer with pH response;

[0060] Step B: adding chemotherapeutic drugs and photodynamic drugs to the aqueous solution of nanocarriers obtained in step A to form a mixed solution, the ...

Embodiment 3

[0064] Blood Compatibility of Nanocarriers:

[0065] Step 1: Take fresh mouse blood, centrifuge at 3000rpm / min for 10 minutes to remove the supernatant to obtain red blood cells, and then wash the product continuously with PBS buffer 5 times;

[0066] Step 2: Dilute red blood cells 10 times with PBS buffer, and then take 0.1ml red blood cell dilution and add them to 0.9ml carrier solutions with concentrations of 25μg / ml, 50μg / ml, 100μg / ml, 200μg / ml, and 400μg / ml Numbered as tubes 1 to 5 in turn, take 0.1ml red blood cell dilution solution and add it to 0.9ml PBS buffer solution and number it as tube No. 6. As a negative control, take 0.1ml red blood cell dilution solution and add it to 0.9ml distilled water and number it as tube No. 7. as a positive control;

[0067] Step 3: Mix the solutions of Nos. 1 to 7 obtained in Step 2 slightly, put them in a 37°C water bath and incubate for 3 hours, and then centrifuge at a speed of 3000rpm / min for 3 minutes;

[0068] Step 4: measure...

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Abstract

The invention discloses a composite nano-drug for integrated tumor diagnosis and treatment and a preparation method thereof. A chemotherapeutic drug, a photodynamic drug and shRNA plasmids capable of reversing multidrug-resistant MDR1 genes of tumor cells are located through a nano-carrier, a magnetic nano-sphere with precious metal nano-particles arranged on the surface serves as a kernel of the nano-carrier, mesoporous silica with the surface modified with amino groups serves as a shell of the nano-carrier, and a polyelectrolyte thin film layer structure with pH response is formed on the surface of the kernel-shell structure through a layer-by-layer self-assembly technology. The composite nano-drug integrates targeted drug delivery, multimode imaging, chemotherapy, photodynamic therapy and gene therapy, can be applied to early diagnosis of tumors, image mediation and real-time monitoring treatment at the same time and overcomes the defect that tumor diagnosis and treatment are separate in the prior art; besides, the composite nano-drug is simple in preparation process, high in operability and suitable for industrial production.

Description

technical field [0001] The invention relates to a multifunctional composite nano-medicine for integration of tumor diagnosis and treatment and a preparation method. Background technique [0002] Tumor is one of the major diseases threatening human health, especially malignant tumors. Malignant tumors are becoming the number one killer that threatens human life. The prevalence of tumors worldwide is gradually increasing every year, and the mortality rate of tumors is also rising. Effective treatment of tumors requires early and accurate detection, so as to achieve timely treatment and improve treatment effects. However, the diagnosis and treatment of tumors has always been a difficult problem in the scientific community. [0003] In the early diagnosis of tumors, it is crucial to use different modalities of imaging techniques to obtain the structural, metabolic and biochemical information of tumors. Tumor diagnosis techniques commonly used clinically include X-ray tomograph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/08A61K49/04A61K49/00A61K31/7088A61K41/00A61K45/00A61K47/02A61K47/04A61K47/36A61K47/48A61P35/00
CPCA61K31/7088A61K41/00A61K41/0057A61K45/00A61K47/02A61K47/36A61K49/0054A61K49/04A61K49/08A61K2300/00
Inventor 刘贻尧陈银杨红吴春惠曾红娟
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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