Double-ion response type SERS (Surface Enhanced Raman Scattering) probe and preparation method thereof

A technology of ion and probe, applied in the field of SERS probe with dual ion response and its preparation, can solve the problems of no similar technology found, no attention to the removal and separation of contaminating ions, single detection types, etc., and achieve good biocompatibility , good response characteristics, and the effect of good magnetic properties

Inactive Publication Date: 2014-03-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These detection technologies have played an important role in the field of heavy metal ion detection, but at the same time, they also encountered many limitations and challenges, such as cumbersome sample preparation, low sensitivity, si

Method used

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  • Double-ion response type SERS (Surface Enhanced Raman Scattering) probe and preparation method thereof
  • Double-ion response type SERS (Surface Enhanced Raman Scattering) probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Detection of mercury ions

[0051] The oligonucleotide chain of the carrier is designed as GTCTGTCTTGACGTC-SH, the oligonucleotide chain of the guest is designed as SH-GATCACTGT CTGTTC, and the Raman molecule of the labeled guest is 4-MBA; the guest sequence is partially paired with the carrier sequence, In this design, except for the unpaired bases T and T, other bases are paired with each other; under the action of mercury ions, a T-Hg-T mismatch structure can be formed between T and T, making the two chains completely paired to form a probe structure with SERS activity.

[0052] Add spare parts D and spare G to the solution of mercury ions to be measured at the same time, and use a shaker to shake for 2 hours; place the solution in an external magnetic field, within 1 minute, the magnetic SERS probes are quickly captured by the external magnetic field, gather and It is separated from the original solution system and forms a magnetically separated precipit...

Embodiment 2

[0055] Embodiment two: detect silver ion

[0056] The oligonucleotide chain of the carrier is designed as TCACCTCACTACGTC-SH, the oligonucleotide chain of the object is designed as SH-GATCAACTCCACTCA, and the Raman molecule of the labeled object is DTNB; the sequence of the object is partially paired with the sequence of the carrier. In this design , except that bases C and C are not paired, other bases are paired with each other; under the action of silver ions, C and C can form a C-Ag-C mismatch structure, so that the two chains are completely paired and form Probe structure with SERS activity.

[0057] Add D spare and G spare to the solution of silver ions to be tested at the same time, and use a shaker to shake for 2 hours; put the solution in an external magnetic field, within 1 minute, the magnetic SERS probe is quickly captured by the external magnetic field, aggregated and It is separated from the original solution system and forms a magnetically separated precipitate...

Embodiment 3

[0060] Embodiment three: detect mercury ion and silver ion

[0061] The oligonucleotide chain of the carrier was designed as SH-CTGCAGTT CTGTCTGTCACTCCACT, the oligonucleotide chain of the object was designed as ACTCACCTCAGGGGG-SH and CTTGTCTGTCAAAA-SH, and the Raman molecules of the labeled object were 4-MBA and DTNB.

[0062] Add spare parts D and spare G to the solution containing both mercury and silver ions at the same time, and shake it for 2 hours with a shaker; place the solution in an external magnetic field, within 1 minute, the magnetic SERS probe is quickly absorbed by the external magnetic field Captured, aggregated and separated out of the original solution system to form a magnetically separated precipitate.

[0063] The magnetically separated precipitate was dissolved in PBS buffer; 20 μL of the solution was dropped onto a glass slide, and placed on a Raman spectrometer detection platform to detect SERS signals; In 15 seconds, import the acquired spectrum into...

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Abstract

The invention discloses a double-ion response type SERS (Surface Enhanced Raman Scattering) probe and a preparation method thereof. The probe comprises two nano particles with independent core-shell structures, wherein a carrier nano particle comprises four layers of core-shell structures; the four layers of core-shell structures are as follows: the innermost layer is made of Fe3O4 magnetic nano balls, a layer of silicon dioxide is coated outside the innermost layer, the second-outer layer is a gold foil shell layer, and the outermost layer is an oligonucleotide coating layer; an object nano particle comprises two layers of core-shell structure; the two layers of core-shell structure are as follows: the inner core is made of Raman molecule marked gold nano-balls, and a shell is an oligonucleotide coating layer. Due to the adoption of a specific base sequence in the oligonucleotide coating layer, the identify and SERS trace detection on two metal ions, namely silver/mercury, in an environment solution can be performed at the same time; furthermore, under the action of an external magnetic field, the probe and the obtained silver/mercury ions can be rapidly separated from an initial environment solution system, and therefore, the function of purifying the environment is realized.

Description

technical field [0001] The invention relates to a dual-ion responsive SERS probe and a preparation method thereof, belonging to heavy metal ion detection and nanometer material preparation technologies. Background technique [0002] Heavy metal ion pollution poses a huge threat to the ecological environment and human health. Mercury ion is a common heavy metal pollution ion, which may cause kidney / liver failure, osteomalacia, joint deformity, nervous system disorders (such as Minamata disease) and other diseases. In addition, it can be bioaccumulated, not easily metabolized, and easily accumulated into the human body through the food chain, which further amplifies its threat to human health. Silver ions are also a common heavy metal ion. A small amount of silver ions is harmless to human health. However, excessive intake of silver ions can also cause great damage to human health. Studies have shown that excessive intake of silver ions may lead to embryonic growth retardati...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y40/00
Inventor 王著元刘敏宗慎飞崔一平钟嫄陈辉朱丹伍磊
Owner SOUTHEAST UNIV
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