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Magnetic composite microsphere with nucleocapsid structure and method for producing the same

A technology of magnetic composite microspheres and core-shell structures, applied in the direction of magnetism of inorganic materials, can solve the problems of reduced production efficiency, cumbersome preparation process, and increased production cost, and achieve controllable magnetic content, simple preparation process, and particle size distribution. narrow effect

Inactive Publication Date: 2008-07-16
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can obtain high magnetic content and narrow distribution magnetic composite microspheres, these methods require multiple steps and special instruments, thus making the preparation process cumbersome, increasing production costs, and reducing production efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of core-shell magnetic composite microspheres with a magnetic content of 76wt%

[0020] 1. Oleic acid modified Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0021] Take 25.0g of FeCl 3 ·6H 2 O and 15.0 g FeCl 2 4H 2 O, dissolved in 50mL of deionized water, transferred to a 500mL four-necked flask, heated to 80°C under nitrogen, quickly added 50mL of ammonia water under rapid stirring, then added 4.0g of oleic acid, reacted at 80°C for 3h , and then pass nitrogen at 80°C for 30 minutes to remove excess ammonia water, then cool down to room temperature, wash with deionized water, ethanol, and propanol to remove excess oleic acid, extract with n-octane, and prepare Fe 3 o 4 The ferrofluid content is 20-70wt%.

[0022] 2. Magnetic composite microsphere Fe 3 o 4 Preparation of / P(MMA / DVB)

[0023] Take 1.0g of magnetic fluid with a magnetic content of 25wt%, wash it with ethanol and acetone, and separate it magnetically, then mix it with ...

Embodiment 2

[0026] Example 2: Preparation of core-shell magnetic composite microspheres with a magnetic content of 66wt%

[0027] 1. Oleic acid modified Fe 3 o 4 The preparation of magnetic nanoparticles is the same as described in Example 1-1.

[0028] 2. Magnetic composite microsphere Fe 3 o 4 / P(MMA / DVB) was prepared as described in Examples 1-2.

[0029] 3. Fe with core-shell structure 3 o 4 / P(MMA / DVB)@P(St / GMA / DVB) composite microspheres were prepared as described in Examples 1-3. The difference is that 1.5g of the shell monomer is added, and the magnetic content of the finally obtained magnetic composite microsphere is 66wt%.

Embodiment 3

[0030] Example 3: Preparation of core-shell magnetic composite microspheres with a magnetic content of 47wt%

[0031] 1. Oleic acid modified Fe 3 o 4 The preparation of magnetic nanoparticles is the same as described in Example 1-1.

[0032] 2. Magnetic composite microsphere Fe 3 o 4 / P(MMA / DVB) was prepared as described in Examples 1-2.

[0033] 3. Fe with core-shell structure 3 o 4 / P(MMA / DVB)@P(St / GMA / DVB) composite microspheres were prepared as described in Examples 1-3. The difference is that 2.0 g of the shell monomer is added, and the magnetic content of the finally obtained magnetic composite microsphere is 47 wt%.

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Abstract

The invention belongs to the technical field of bioseparation material, in particular to a magnetic composite microsphere with nucleocapsid structure and a preparation method thereof. Nano ferroferric oxide decorated by oleic acid is firstly used as raw material, and is polymerized through template fine emulsion so as to prepare the monodisperse magnetic composite microsphere with high magnet content; and then the monodisperse micosphere is polymerized through seed emulsion and is added with different amount of shell monomer to prepare the magnetic composite microsphere with the nucleocapsid structure and the magnet content of 20wt percent to 80wt percent. The grain size of nucleocapsid composite microsphere is in narrow distribution, and the magnet content of the microsphere is controllable and the surface has functional reactive group; therefore, the microsphere of the invention can be surface-modified into a magnetic carrier of biological active molecule by chemical crosslinking to be applied in the biomedicine field. The method of the invention can be simply operated and the process of which can be controlled, thus having industrialized prospect.

Description

technical field [0001] The invention belongs to the technical field of biological separation materials, and in particular relates to a preparation method of magnetic composite microspheres with a core-shell structure. technical background [0002] In recent years, organic-inorganic hybrid composite microspheres, especially magnetic composite microspheres, have attracted more and more attention. Since magnetic polymer microspheres have both the magnetic responsiveness of inorganic magnetic substances and the surface functionality of organic polymers, they can easily, quickly and efficiently separate target biomolecules from the medium under an external magnetic field. In addition, the polymer surface of magnetic composite microspheres can be purposefully and rationally modified, so it shows broad application prospects in the fields of medicine, biochemistry, molecular biology and industrial applications. From the perspective of diagnostics, ideal magnetic microspheres should...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L25/04C08L33/12C08F12/08C08F20/14C08F2/22C08F2/44C08K9/04H01F1/11C08K3/22
Inventor 龚涛汪长春
Owner FUDAN UNIV
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