Method for preparing magnetic liposome

A magnetic liposome and liposome technology, which are applied in the directions of liposome delivery, medical preparations of inactive ingredients, pharmaceutical formulations, etc., to achieve the effects of increasing the encapsulation capacity and increasing the magnetic content

Inactive Publication Date: 2011-08-24
NANTONG CAMBRIDGE OLEIN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The magnetic liposome structure disclosed in the above documents is Figure 2 shows that liposome 3 encapsulates magnetic particles 2 and drug 1 inside, and this structure has some limitations
First, the magnetic content is not high and the magnetic response is low
Due to the limited internal space of liposomes, especially the phospholipid bilayers of small unilamellar liposomes (SUV), only a small amount of magnetic particles can be enclosed in liposomes; Sedimentation, when liposomes are hydrated to form vesicles, it is difficult to evenly coat them
Second, the drug loading is low
Due to the limited internal space of liposomes, when magnetic particles occupy them, the drug loading capacity decreases

Method used

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  • Method for preparing magnetic liposome

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Egg yolk lecithin and cholesterol were dissolved in methanol-chloroform mixed organic solvent (1:2, v / v) respectively to obtain 0.1g / mL egg yolk lecithin solution and 0.1g / mL cholesterol solution, and 10mL of egg yolk lecithin solution and 2.5mL of cholesterol solution were mixed in an eggplant-shaped bottle, and rotovaped to form a liposome film. Add 50mL of phosphate buffer solution (pH, 7.4) into the eggplant-shaped bottle forming the liposome film, use a vortex mixer to vortex for 20min, use a high-speed dispersing homogenizer to disperse for 5min, and centrifuge and wash twice with water after fully hydrating to obtain a surface Negatively charged liposomes. Disperse 1 g of the above-mentioned negatively charged liposomes in 60 mL of polydiallyldimethylammonium chloride aqueous solution with a concentration of 0.8 mg / (ml 0.5 M NaCl), let stand for adsorption for 20 min, and then centrifuge and wash with water; redisperse In 60mL of polystyrene sodium su...

Embodiment 2

[0025] Embodiment 2: Egg yolk lecithin and cholesterol are dissolved in methanol-chloroform mixed organic solvent (1: 2, v / v) respectively to obtain 0.1g / mL egg yolk lecithin solution and 0.1g / mL cholesterol solution. 10mL of egg yolk lecithin solution and 2mL of cholesterol solution were mixed in an eggplant-shaped bottle, and the liposome film was formed by rotary evaporation. Add 60mL of phosphate buffer solution (pH, 7.4) into the eggplant-shaped bottle forming the liposome film, use a vortex mixer to vortex for 30min, use a high-speed dispersing homogenizer to disperse for 6min, and centrifuge and wash 3 times with water after fully hydrating to obtain a surface Negatively charged liposomes. Disperse 1 g of the above-mentioned negatively charged liposomes on the surface in 70 mL of polydiallyldimethylammonium chloride aqueous solution with a concentration of 1 mg / (ml 0.5 M NaCl), let stand for adsorption for 20 min, and then wash with centrifugal water; then disperse in ...

Embodiment 3

[0026] Example 3: Egg yolk lecithin and cholesterol were dissolved in methanol-chloroform mixed organic solvent (1:2, v / v) respectively to obtain 0.1g / mL egg yolk lecithin solution and 0.1g / mL cholesterol solution, and 10mL of egg yolk lecithin solution and 2mL of cholesterol solution were mixed in an eggplant-shaped bottle, and the liposome film was formed by rotary evaporation. Add 70mL of phosphate buffer solution (pH, 7.4) into the eggplant-shaped bottle forming the liposome film, use a vortex mixer to vortex for 30min, use a high-speed dispersing homogenizer to disperse for 8min, centrifuge and wash twice with water after fully hydrating to obtain a surface Negatively charged liposomes. Disperse 1 g of the above-mentioned negatively charged liposomes on the surface in 70 mL of polydiallyldimethylammonium chloride aqueous solution with a concentration of 1.2 mg / (ml 0.5 M NaCl), let stand for adsorption for 30 min, then centrifuge and wash with water; redisperse In 70mL of...

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Abstract

The invention discloses a method for preparing a magnetic liposome. The medicine is coated inside the magnetic liposome; and magnetic particles are coated outside the liposome. The preparation method comprises the following steps that: a yolk lecithin solution and a cholesterin solution are mixed in an eggplant-shaped bottle according to the molar ratio of 2:1 to 2.5:1; the mixture is subjected to rotary evaporation to form a liposome film; then a phosphate buffering solution is used to carry out full hydration on the liposome film to obtain the liposome of which the surfaceis provided with negative charges; then, cationic polyelectrolyte-poly diallyl dimethyl ammonium chloride, anionic polyelectrolyte which is sodium polystyrene sulfonate and cationic polyelectrolyte which is poly diallyl dimethyl ammonium chloride are subjected to alternate adsorption; and finally, through the charge action and a self-assembling method, Fe3O4 magnetic particles of which the surfaces are provided with negative charges are deposited on the surface of the liposome to obtain the magnetic liposome. As the inside of the liposome is packed with the medicine, the magnetic particles are deposited on the surface of the liposome and a finite space of the inner space of the liposome is totally packed with the medicine, the packing amount of the medicine is improved and the magnetic content is simultaneously improved.

Description

technical field [0001] The invention relates to a preparation method of liposome, in particular to a preparation method of magnetic liposome. Background technique [0002] The Chinese invention patent of document 1 "publication number is CN100998563" discloses a preparation method of drug-loaded magnetic liposomes. First, doxorubicin drug is dissolved in the prepared magnetic fluid to form doxorubicin magnetic fluid. L-alpha-phosphatidylethanolamine, cholesterol, L-alpha-phosphatidylcholine make liposome film by the weight ratio of 7: 2: 1, then add doxorubicin magnetic fluid into the solanum that forms liposome film In a shaped bottle, vortex at room temperature for 30 minutes until the film comes off completely, pour the initial solution into a test tube, perform ultrasonic pulverization with intermittent pulses for 20 minutes, and let stand for 12 hours to form magnetic doxorubicin liposomes. [0003] Document 2, the Chinese invention patent with publication number CN15...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/32A61K47/20A61K47/16A61K9/127A61K47/28A61K47/02
Inventor 赵雯卢婷利张宏陈涛于洋
Owner NANTONG CAMBRIDGE OLEIN
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