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A preparation method and application of boron ester-rich core-shell magnetic composite microspheres

A magnetic composite microsphere, core-shell technology, applied in the magnetic direction of organic materials/organic magnetic materials, can solve the problems of inability to enrich glycoprotein markers, adsorption, etc., and achieve excellent results, uniform particle size distribution, and hydrophilic good sex effect

Inactive Publication Date: 2017-07-07
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this enrichment will bring a certain amount of non-specific adsorption, because boric acid itself is hydrophobic, and when it is connected to the material, it will make the material have a certain degree of hydrophobicity, so that hydrophobic non-glycoproteins may be adsorbed during enrichment
In addition, the enrichment is carried out under alkaline conditions, and the environment in the human body is neutral. To enrich the most original glycoproteins in the human body, alkaline enrichment will often make some Stable glycoproteins decompose or denature, and cannot be enriched to obtain the most original glycoprotein markers in the human body

Method used

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  • A preparation method and application of boron ester-rich core-shell magnetic composite microspheres
  • A preparation method and application of boron ester-rich core-shell magnetic composite microspheres
  • A preparation method and application of boron ester-rich core-shell magnetic composite microspheres

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Embodiment 1

[0034] Embodiment 1: shell layer thickness is about 10nm, and crosslinking degree is the core-shell type Fe of 20% 3 o 4 The preparation of / PAA-AOPB microspheres, concrete steps are as follows:

[0035] (1), preparation of magnetic clusters stabilized by sodium citrate

[0036] 1.3g ferric chloride hexahydrate (FeCl 3 •6H 2 O), 3.8g ammonium acetate (NH 4 Ac), after dissolving 0.4g sodium citrate in 70mL ethylene glycol, put it into a 150mL three-neck flask, then raise the temperature to 170°C, stir and react for 1 hour, then transfer the liquid in the flask into a 100mL polytetrafluoroethylene-containing Lined autoclave, then put the autoclave into an oven at 200°C for 16 hours, take it out, and cool it to room temperature with tap water. The product is separated by magnetic separation, washed with absolute ethanol to remove unreacted reactants, and finally the product is dispersed in absolute ethanol for later use.

[0037] (2) Active vinyl modification on the surface...

Embodiment 2

[0043] Example 2: Core-shell Fe with a shell thickness of about 30 nm and a crosslinking degree of 20% 3 o 4 Preparation of / PAA-AOPB microspheres (transmission electron microscope photos see figure 1 )

[0044] 1. The preparation of sodium citrate-stabilized magnetic clusters is the same as that described in step (1) of Example 1.

[0045] 2. Carrying out active vinyl modification on the surface of the magnetic cluster is the same as described in the step (20) of Example 1.

[0046] 3. Core-shell Fe 3 o 4 The preparation of / PAA is the same as described in Example 1 step (3). The difference is that acrylic, N, N' The dosages of -methylenebisacrylamide and 2,2-azobisisobutyronitrile are 400 μL, 100 mg, and 10 mg, respectively.

[0047] 4. The reaction of modifying aminophenyl borate is the same as that described in step (4) of Example 1.

Embodiment 3

[0048] Example 3: The shell thickness is about 50nm, and the cross-linking degree is 20% core-shell Fe 3 o 4 / Preparation of PAA-AOPB Microspheres

[0049] 1. The preparation of sodium citrate-stabilized magnetic clusters is the same as that described in step (1) of Example 1.

[0050] 2. Carrying out active vinyl modification on the surface of magnetic clusters is the same as that described in step (2) of Example 1.

[0051] 3. Core-shell Fe 3 o 4 / PAA is prepared as described in Example 1 step (3). The difference is that acrylic, N, N' The dosages of -methylenebisacrylamide and 2,2-azobisisobutyronitrile are 600 μL, 150 mg, and 15 mg, respectively.

[0052] 4. The reaction of modifying aminophenyl borate is the same as described in the embodiment step (4).

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Abstract

The invention relates to a preparation method and application of a core-shell magnetic composite microsphere that can be used for separating glycoproteins. The core of the core-shell magnetic composite microsphere of the present invention is a cluster of magnetic ferric iron tetroxide nanoparticles, and the shell is a cross-linked polymer network rich in carboxyl groups, and then the carboxyl group and aminophenyl boron ester amidation reaction for surface modification, through A large number of boron esters immobilized on the surface can rapidly separate and enrich glycoproteins under physiological conditions. Firstly, sodium citrate-stabilized magnetic nanoparticle clusters were prepared, followed by the sol-gel method to modify the surface of the magnetic clusters with active vinyl functional groups, and then a highly magnetically responsive monodisperse surface rich in carboxyl groups was prepared by reflux precipitation polymerization The core-shell magnetic polymer composite microspheres were modified with aminophenylboronate and carboxyl groups for amidation reaction to modify a large number of boronate groups, and finally the separation and enrichment of glycoproteins was carried out. The method of the invention is simple, the process is controllable, the efficiency of separating and purifying glycoprotein is high, and it can be separated and enriched under physiological conditions.

Description

technical field [0001] The invention belongs to the technical field of nanometer functional materials, and in particular relates to a preparation method and application of core-shell magnetic composite microspheres rich in boron esters. Background technique [0002] In recent years, organic-inorganic hybrid composite microspheres, especially magnetic composite microspheres, are receiving widespread attention. Since magnetic polymer microspheres have both the magnetic responsiveness of inorganic magnetic materials and the surface modifyability of organic polymers, they can separate target biomolecules conveniently, quickly and efficiently under an external magnetic field. Therefore, it has broad application prospects in biomedical fields such as protein separation and purification, cell separation, magnetic resonance detection, and magnetically targeted drug loading. [0003] At present, magnetic polymer composite microspheres with different structures can be prepared by emu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F292/00C08F8/42H01F1/42C07K1/14
Inventor 汪长春章雨婷
Owner FUDAN UNIV
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