Method for preparing nuclear shell type high magnetic content super paramagnetic microsphere

A superparamagnetic, core-shell technology, applied in the direction of magnetism of inorganic materials, to achieve good commercial application prospects, uniform particle size, and less production process steps

Inactive Publication Date: 2011-04-06
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Has an organic-organic composite core-shell structure, especially the preparation process of the core-shell magnetic microsphere material with an acrylamide-acrylic acid copolymer shell and a urea-formaldehyde magnetic microsphere core has not yet been reported.

Method used

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  • Method for preparing nuclear shell type high magnetic content super paramagnetic microsphere
  • Method for preparing nuclear shell type high magnetic content super paramagnetic microsphere
  • Method for preparing nuclear shell type high magnetic content super paramagnetic microsphere

Examples

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Effect test

example 1

[0037] Preparation of monoclonal antibody magnetic immune microspheres:

[0038] Prepare ferrofluid:

[0039] Add nitric acid solution, 0.2g / ml urea solution and ultrapure water in sequence to the ferric oxide powder, and mix them evenly to form a mixture; the magnetic powder, acid solution, urea solution and ultrapure water in the mixture The mass ratio of the mixture is 0.3:1:1.8:16.5; the mixture is ultrasonically dispersed in a water bath at 70°C for 20 minutes, and then placed at 20°C for 7 days to sink and purify to remove impurities;

[0040] Prepare magnetic microsphere suspension:

[0041] Take the purified magnetic fluid, adjust the pH value to 5.5 with sodium hydroxide solution, add formaldehyde solution with a volume percentage concentration of 37% therein, and the volume ratio of the magnetic fluid and the formaldehyde solution is 40:1; then place the mixed solution Stand still for 30 minutes in a water bath at 50°C. After observing a large number of microsphere...

example 2

[0049] Prepare ferrofluid:

[0050] In the iron nitride powder, sequentially add hydrochloric acid solution, 0.01g / ml urea solution and ultrapure water with a mass percentage concentration of 1%, and mix uniformly to form a mixture; the magnetic powder, acid solution, urea solution and ultrapure water in the mixture The mass ratio is 0.3:1:1.8:16.5; the mixture is ultrasonically dispersed in a water bath at 70°C for 20 minutes, and then placed at 20°C for 7 days, and purified to remove impurities;

[0051] Prepare magnetic microsphere suspension:

[0052] Take the purified magnetic fluid, adjust the pH value to 7.0 with potassium hydroxide solution, add a formaldehyde solution with a concentration of 37% by volume, and the volume ratio of the magnetic fluid to the formaldehyde solution is 30:1; In a water bath environment at 10°C, let it stand for 40 minutes. After observing a large number of microspheres with a particle size of 1um under a microscope, disperse the product in...

example 3

[0056] Prepare ferrofluid:

[0057] Add the perchloric acid solution, 0.5g / ml urea solution and ultrapure water in sequence to the ferric oxide powder, and mix them evenly to form a mixture; the magnetic powder, acid solution, urea solution and ultrapure water in the mixture The mass ratio of pure water is 0.3:1:1.8:16.5; the mixture is ultrasonically dispersed in a 70°C water bath for 20 minutes, and then placed at 20°C for 7 days to purify and remove impurities;

[0058] Prepare magnetic microsphere suspension:

[0059] Take the purified magnetic fluid, adjust the pH value to 4.0 with ammonia water, add a formaldehyde solution with a volume percentage concentration of 37% to it, and the volume ratio of the magnetic fluid to the formaldehyde solution is 45:1; then place the mixed solution at 70°C In a water bath environment, stand still for 5 minutes. After a large number of microspheres with a particle size of 1um are observed under a microscope, the product is dispersed in...

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Abstract

The invention discloses a method for preparing nuclear shell type high magnetic content super paramagnetic microsphere. Magnetic powder is treated at certain temperature with nitric acid and urea to obtain magnetic fluid; a certain amount of formaldehyde is added to solidify the magnetic fluid under the condition of water bath after the magnetic fluid is aged for days, and thus, magnetic microspheres are obtained; a proper amount of dispersant, acrylamide and crylic acid are added to the suspension of the magnetic microsphere to prepare the mixed liquid, and then the mixed liquid stands for one day in low temperature; then a certain amount of absolute ethyl alcohol and diisopropyl azodicarboxylate are added to the mixed liquid to carry out water bath heating and deoxidation processes; thetemperature is regulated to initiate reaction after PH value is regulated to neutrality; a product is washed after the reaction, and thus, the final product-magnetic microsphere with acrylamide-acrylic copolymer shell is obtained. The obtained product-nuclear shell type magnetic microsphere of the invention has the characteristics of high magnetic content, uniform grain diameter, few production process steps and low price of raw material and can be cross-linked with antibodies to prepare various types of immune diagnostic reagents.

Description

technical field [0001] The invention relates to a method for preparing core-shell superparamagnetic microspheres with high magnetic content. Background technique [0002] Magnetic polymer microspheres refer to microspheres with certain magnetic properties and special structures synthesized from organic polymer-inorganic magnetic material composite materials. Magnetic polymer microspheres have many characteristics of both magnetic and polymer materials: not only can surface functional groups be added through copolymerization and surface modification, but also have magnetic responsiveness, and can be separated and manipulated under the action of an external magnetic field. Therefore, magnetic polymer microsphere materials have shown strong vitality in many application fields such as biomedical engineering, cell separation and medical detection, and several commercial magnetic separation and analysis products have come out. [0003] Magnetic polymer microspheres are mainly cla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01F1/26C08F220/56C08F220/06C08F2/44
Inventor 吴宥伸张楠吴道澄
Owner XI AN JIAOTONG UNIV
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