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Preparation method of superparamagnetic composite microballoons used in biomedicine

A technology of superparamagnetic and composite microspheres, which is applied to the magnetism of organic materials/organic magnetic materials, scientific instruments, instruments, etc., which can solve the problems of complex detection process, high detection cost, and great pain for patients, and achieve simple preparation process , wide application prospects, the effect of high magnetic content

Inactive Publication Date: 2012-10-10
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection techniques for the detection of diffuse tumor cells include fluorescent labeling method, HE staining method, immunohistochemical method, PCR technology, flow cytometry and other detection methods. High requirements, high testing costs, and great pain to patients

Method used

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  • Preparation method of superparamagnetic composite microballoons used in biomedicine
  • Preparation method of superparamagnetic composite microballoons used in biomedicine
  • Preparation method of superparamagnetic composite microballoons used in biomedicine

Examples

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Effect test

Embodiment 1

[0022] Embodiment one: prepare 100mL standard FeCl respectively by 0.5mol / L 3 ·6H 2 O and FeCl 2 4H 2 O solution, take 63mL Fe 3+ solution and 37mL Fe 2+ The solution was placed in a 250ml four-neck flask. Stir at high speed under the protection of nitrogen, heat the system to 80°C in a water bath, quickly add concentrated ammonia water, and adjust the pH of the system to 10. At this time, the system immediately turns black, and a large amount of black Fe is produced by hydrolysis. 3 o 4 Nanoparticles. After reacting for 1 h, continue to pass nitrogen gas until the system is cooled to room temperature, and wash with deionized water several times until the pH value of the supernatant reaches neutral. Weigh the Fe with a net content of 3g 3 o 4 Disperse nanoparticles into 100mL deionized water, heat up to 80°C under nitrogen protection, add 1.34g oleic acid, react for 40min, cool to room temperature, add 0.67g sodium dodecylbenzenesulfonate, stir for half an hour, and ...

Embodiment 2

[0023] Embodiment 2: The difference between this embodiment and Embodiment 1 is: prepare 100mL standard FeCl at 0.5mol / L respectively 3 ·6H 2 O and FeCl 2 4H 2 O solution, take 60mL Fe 3+ solution and 40mL Fe 2+ The solution was placed in a 250ml four-neck flask. Stir at high speed under the protection of nitrogen, heat the system to 50°C in a water bath, quickly add concentrated ammonia water, and adjust the pH of the system to 9. At this time, the system immediately turns black, and a large amount of black Fe is produced by hydrolysis. 3 o 4 Nanoparticles. After reacting for 1 h, continue to pass nitrogen gas until the system is cooled to room temperature, and wash with deionized water several times until the pH value of the supernatant reaches neutral. Weigh the Fe with a net content of 3g 3 o 4 Disperse nanoparticles in 100mL deionized water, heat up to 60°C under nitrogen protection, add 1.67g oleic acid, react for 30min, cool to room temperature, add 0.5g sodiu...

Embodiment 3

[0024] Embodiment three: the difference between this embodiment and embodiment one is: prepare 100mL standard FeCl respectively by 0.5mol / L 3 ·6H 2 O and FeCl 2 4H 2 O solution, take 66.5mL Fe 3+ solution and 33.5mL Fe2+ The solution was placed in a 250ml four-neck flask. Stir at high speed under the protection of nitrogen, heat the system to 75°C in a water bath, quickly add concentrated ammonia water, and adjust the pH of the system to 11. At this time, the system immediately turns black, and a large amount of black Fe is produced by hydrolysis. 3 o 4 Nanoparticles. After reacting for 60 minutes, continue to pass nitrogen gas until the system is cooled to room temperature, and wash with deionized water several times until the pH value of the supernatant reaches neutral. Weigh the Fe with a net content of 3g 3 o 4 Disperse nanoparticles into 100mL deionized water, heat up to 70°C under nitrogen protection, add 0.67g oleic acid, react for 1h, cool to room temperature,...

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Abstract

The invention relates to a preparation method of superparamagnetic composite microballoons used in biomedicine. First, a coprecipitation method is used to prepare superparamagnetic Fe3O4 nanoparticles, and after modified by a surfactant, the prepared Fe3O4 nanoparticles are dispersed into deionized water to form a water-based magnetic liquid. Second, inorganic / organic core shell microballoons with carboxyl function groups on surfaces are prepared. The superparamagnetic composite microballoons are of an inorganic / organic core shell structure, have composite material characteristics and biological effects. The microballoons have active function groups on the microballoon surfaces, and therefore can be combined with a plurality of biologically-active substances. The magnetic microballoons prepared by the method of the invention have active carboxylic groups on the microballoon surfaces, and have superparamagnetism and a large magnetic content, and therefore the microballoons are subject to magnetization separation under an externally applied magnetic field and can lose magnetism immediately after the magnetic filed is removed. The magnetic microballoons prepared in the invention can be widely applied in the detection and separation operation of tumor cells in the field of biomedicine. The method has simple experimentation, is rapid, and has low cost.

Description

technical field [0001] The invention relates to a method for preparing functional superparamagnetic composite microspheres for tumor cell detection in combination with microbial technology and polymer magnetic microspheres. Background technique [0002] For cancer patients, there will be a small amount of cancer cells in the tissue and blood when the cells are lesioned in the early stage and cancer cells are formed. If cell cancer can be detected early, the cure rate can reach 80%, which is the authoritative conclusion of the World Health Organization. Therefore, timely and simple detection of trace cancer cells in circulating blood is of great significance to prevent tumor cells from continuing to spread and treat cancer patients. [0003] At present, the detection techniques for the detection of diffuse tumor cells include fluorescent labeling method, HE staining method, immunohistochemical method, PCR technology, flow cytometry and other detection methods. The requireme...

Claims

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Application Information

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IPC IPC(8): C08F292/00C08F212/08C08F220/14C08F220/06H01F1/42G01N33/531
Inventor 杨冬芝于坤幺崇正聂俊
Owner BEIJING UNIV OF CHEM TECH
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