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Preparing method used for nanometer magnetic composite microspheres for protein separation and purification

A protein separation and nano-magnetic technology, applied in the field of preparation of nano-magnetic composite microspheres, can solve the problems such as the need to improve the dispersion stability of the microspheres, the unsatisfactory monodispersity of the microspheres, and the immature preparation technology, thereby eliminating the need for centrifugation. , The effect of strong magnetic responsiveness and high magnetic content

Active Publication Date: 2011-08-17
SUNDIA MEDITECH COMPANY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of magnetic microspheres by monomer copolymerization has been reported in the literature, but the preparation technology is not yet mature, the monodispersity of the prepared microspheres is not ideal, the magnetic response is weak, and the biochemical activity and dispersion stability of the microspheres are not good enough. It needs to be improved, which limits its application in protein separation and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of Fe by co-precipitation method 3 o 4 Nano magnetic particles, take 16g FeCl 3 and 19gFeSO 4 ·7H 2 O, dissolved in 50ml of deionized water, then added to a 250ml three-necked flask, under the protection of nitrogen, at 300rpm, the temperature was raised to 70°C, then NaOH was added to adjust the pH value to 8-9, and the reaction was continued for half an hour, and then deionized water , washed three times with absolute ethanol, and finally the Fe 3 o 4 The nano magnetic particles are dispersed in absolute ethanol to form a mixed solution with a solid content of 50 wt%. Take 40g of the mixed solution, add 4g of vinyltriethoxysilane, 20g of deionized water, and adjust the pH value of the mixed solution to 5.5 with acetic acid, heat in a three-necked flask equipped with a reflux condenser, a mechanical stirrer, and nitrogen gas After reacting at a constant temperature of 70°C for 2 hours, wash with deionized water several times, magnetically separate, an...

Embodiment 2

[0025] The operation method and conditions are the same as those in Example 1, except that the silane coupling agent is γ-(methacryloyloxy)propyltrimethoxysilane.

Embodiment 3

[0027] The operating method and conditions are the same as in Example 1, except that the silane coupling agent is vinyltrimethoxysilane.

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Abstract

The invention relates to a preparing method used for utilizing protein to separate and purify nanometer magnetic composite microspheres. The preparing method comprises the following steps of: preparinThe invention relates to a preparing method used for utilizing protein to separate and purify nanometer magnetic composite microspheres. The preparing method comprises the following steps of: preparing magnetic nanoparticles, then adopting a silane coupling agent to modify the surfaces of the nanoparticles, mixing and dispersing the modified particles and high-molecular monomers, a cross linker ang magnetic nanoparticles, then adopting a silane coupling agent to modify the surfaces of the nanoparticles, mixing and dispersing the modified particles and high-molecular monomers, a cross linker and an initiator in water phase containing a surface active agent, carrying out emulsion polymerization and obtaining the magnetic composite microspheres. The prepared nanometer magnetic composite microd an initiator in water phase containing a surface active agent, carrying out emulsion polymerization and obtaining the magnetic composite microspheres. The prepared nanometer magnetic composite microspheres have particle diameters between 50 to 500 nanometers, rich surface function groups, good dispersion stability in medium, high efficiency of immune ligand coupling and strong magnetic responsivspheres have particle diameters between 50 to 500 nanometers, rich surface function groups, good dispersion stability in medium, high efficiency of immune ligand coupling and strong magnetic responsiveness, and are applicable to fast separation and purification of protein in biological samples.eness, and are applicable to fast separation and purification of protein in biological samples.

Description

technical field [0001] The invention relates to the preparation of nano products, in particular to a preparation method of nano magnetic composite microspheres applied to protein separation and purification. Background technique [0002] Separation, purification and detection of various proteins from samples is an important link in life science and clinical medicine. Traditional protein separation methods such as salting out, organic solvent precipitation, membrane separation technology, and column chromatography technology, etc., are achieved through Change the pH value, temperature, ionic strength, dielectric constant and other factors to achieve the purpose of separation. However, there are some defects in the above methods, such as complicated separation process, high instrument cost, large protein loss, etc., which limit their application. Purification of protein using magnetic microspheres as a solid phase medium is an emerging protein separation and purification tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F292/00C08F2/24C08K9/06C07K1/14
Inventor 王晓川贺枰高峰盛蓉生
Owner SUNDIA MEDITECH COMPANY LTD
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