36 results about "Ligand coupling" patented technology

The present invention includes methods for preparing resveratrol, resveratrol esters and substituted and unsubstituted stilbenes of the formula given below; where each Y is —O or halogen, each Z is —O or halogen, each n and each m is independently the value of 0, 1, 2, 3, 4 or 5, each A and each B is independently selected from Pn, R or absent, each V and each W is independently selected from Pn, straight or branched alkyl of from (2) to (6) carbon atoms and cycloalkyl of from (3) to (8) carbon atoms, alkoxy, phenyl, benzyl or halogen, R is independently selected from the group comprising alkyl with at least one carbon atom, aryl and aralkyl, Pn is an alcohol protecting group and diastereoisomers of the foregoing. The compounds are made from a multi-step process including a N-heterocyclic carbene-type ligand coupling in the presence of a base with benzyol halide and styrene coupling partners. These compounds show increased stability for use in the food, cosmetic and pharmaceutical industries.

Passivated substrates are provided for use in assays, comprising at least one covalently bonded ligand having specific binding activity for a molecule, and at least one covalently bonded blocking agent, wherein said ligand is directly bonded to the substrate surface. In certain embodiments, the ligand and blocking agent are covalently bonded only to the substrate surface, and not directly bonded to each other. In certain other embodiments, the ligand and blocking agent have at least one additional covalent bond to one another. Methods for preparing and using passivated substrates in bioassays are also provided.

Described herein are compounds, pharmaceutical compositions and methods for treating pathogenic cell populations in a patient. The compounds described herein include conjugates of a plurality of cytotoxic drugs and vitamin receptor binding ligands. The plurality of drugs may be the same or different. Similarly, the vitamin receptor binding ligands may be the same or different. The conjugates also include a linker that is formed from one or more spacer linkers, heteroatom linkers, and releasable linkers.

The invention provides methods and systems for predicting or evaluating protein-ligand coupling specificities. A pattern recognition model can be trained by selected sequence segments of training proteins which have a specified ligand coupling specificity. Each selected sequence segment is believed to include amino acid residue(s) that may contribute to the ligand coupling specificity of the corresponding training protein. Sequence segments in a protein of interest can be similarly selected and used to query the trained model to determine if the protein of interest has the same ligand coupling specificity as the training proteins. In one embodiment, the pattern recognition model employed is a hidden Markov model which is trained by concatenated cytosolic domains of GPCRs which have interaction preference to a specified class of G proteins. This trained model can be used to evaluate G protein coupling specificity of orphan GPCRs.

The invention discloses a preparation method for a phenylboronic group modified macroporous resin applicable to separation of rebaudiodside A and stervioside. The method comprises the following main steps: (1) pretreatment of a resin: a step of putting a carboxyl resin in an organic solvent for standing and swelling; (2) activation of the resin: a step of adding activation reagents consisting of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxylsuccimide and carrying out activation; (3) ligand coupling: a step of adding a 3-aminophenylboric acid solution and carrying out a reaction at a constant temperature for 6 to 24 h; and (4) post-treatment of the modified resin: a step of carrying out filtering after completion of the reaction and successively carrying out washing with acetone and deionized water so as to obtain the phenylboronic group modified macroporous resin. The invention has the following beneficial effects: a chromatographic column is filled with the prepared phenylboronic group modified macroporous resin; and the purity of separated rebaudiodside A is more than 90%, and the recovery rate of rebaudiodside A is more than 70%.

An immunoaffinity column for simultaneous analysis of four mycotoxins (DON, ZEA, T-2, HT-2) and a preparation method thereof belong to the preparation techniques. The method includes the preparation and purification of antibodies, the preparation of a matrix, ligand coupling and column packing. The invention has novel design, advanced technology, unique method and wide purpose, not only solves the problems of matrix interference, low specificity and high cost in the traditional enrichment process of trichothecene mycotoxins, but also achieves simultaneous purification of multiple targets. The immunoaffinity column has the advantages of strong specificity, great improvement of the use efficiency, easy operation, low production cost, and strong practicality and generalization performance.

The present disclosure relates to conjugated quantum dot nanoparticles, to methods of making such conjugated quantum dot nanoparticles, and to methods of detecting DNA methylation using such conjugated quantum dot nanoparticles. In one embodiment, the quantum dot is conjugated to an antibody that recognizes methylated DNA bases, such as an anti-5-methylcytosine antibody, an anti-5- hydroxymethylcytosine antibody, an anti-5-formylcytosine antibody, an anti-5-carboxylcytosine antibody or an anti-N6-methyladenine antibody.

This invention relates to a hybrid ligand, a hybrid biomimetic chromedia and a preparing method and a use thereof, wherein the hybrid biomimetic chromedia takes hydrophilic porous microsphere as a substrate in chromatography, activated with allyl bromide and undergoing bromo-alcoholization with N-bromosuccinimide, then coupled with the hybrid ligands. The sequence of the hybrid ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole. The hybrid biomimetic chromedia has both of the two functional groups of phenylalanine-tyrosine-glutamine tripeptide and aminobenzimidazole, while maintaining the high antibody selectivity of polypeptide ligand, hydrophobic electric charge inductive ligand is introduced to achieve more moderate elution requirement, realizing effective antibody separation.

The present invention relates to a filtering device (8) for removing substances from blood or a blood component, the filtering device (8) comprising: a housing having an inlet and an outlet, at first sorbent material coupled with at least a first ligand located within the housing, and a second sorbent material coupled with at least a second ligand located within the housing, wherein the first ligand is for removing free hemoglobin (fHb) and the second ligand is for removing microvesicles (MV) from the blood or blood component passing through the filtering device (8), from the inlet to the outlet, wherein the first and second ligand are different from each other, and wherein the first and second sorbent material are the same or are different from each other.

This invention relates to a hybrid ligand, a hybrid biomimetic chromedia and a preparing method and a use thereof, wherein the hybrid biomimetic chromedia takes hydrophilic porous microsphere as a substrate in chromatography, activated with allyl bromide and undergoing bromo-alcoholization with N-bromosuccinimide, then coupled with the hybrid ligands. The sequence of the hybrid ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole. The hybrid biomimetic chromedia has both of the two functional groups of phenylalanine-tyrosine-glutamine tripeptide and aminobenzimidazole, while maintaining the high antibody selectivity of polypeptide ligand, hydrophobic electric charge inductive ligand is introduced to achieve more moderate elution requirement, realizing effective antibody separation.

The invention relates to a preparing method used for utilizing protein to separate and purify nanometer magnetic composite microspheres. The preparing method comprises the following steps of: preparinThe invention relates to a preparing method used for utilizing protein to separate and purify nanometer magnetic composite microspheres. The preparing method comprises the following steps of: preparing magnetic nanoparticles, then adopting a silane coupling agent to modify the surfaces of the nanoparticles, mixing and dispersing the modified particles and high-molecular monomers, a cross linker ang magnetic nanoparticles, then adopting a silane coupling agent to modify the surfaces of the nanoparticles, mixing and dispersing the modified particles and high-molecular monomers, a cross linker and an initiator in water phase containing a surface active agent, carrying out emulsion polymerization and obtaining the magnetic composite microspheres. The prepared nanometer magnetic composite microd an initiator in water phase containing a surface active agent, carrying out emulsion polymerization and obtaining the magnetic composite microspheres. The prepared nanometer magnetic composite microspheres have particle diameters between 50 to 500 nanometers, rich surface function groups, good dispersion stability in medium, high efficiency of immune ligand coupling and strong magnetic responsivspheres have particle diameters between 50 to 500 nanometers, rich surface function groups, good dispersion stability in medium, high efficiency of immune ligand coupling and strong magnetic responsiveness, and are applicable to fast separation and purification of protein in biological samples.eness, and are applicable to fast separation and purification of protein in biological samples.

Provided is a material for organic electroluminescence device containing (a) a phosphorescent metal complex containing a monoanionic bidentate ligand and a metal having an atomic weight of 40 or greater, wherein the monoanionic bidentate ligand is represented by the following formula (A):wherein, each of E1a, E1d, E1e, E1f, E1k, E1l, E1p, and E1q independently represents a carbon atom or a hetero atom, each of Z1, Z2, and Z3 independently represents an aromatic ring or aromatic heterocycle, the aromatic ring or aromatic heterocycle may have a substituent, and a skeleton represented by the formula (A) has, in total, a 18π electron structure, and the bidentate ligand represented by the formula (A) may be coupled to another ligand to form a tridentate or higher-dentate ligand; and (b) a structural isomer represented by the same chemical formula as that of the phosphorescent metal complex (a) but different sterically in a coordination method of the ligand, wherein the structural isomer (b) is contained in an amount of 0.05 mass % or greater but not greater than 5 mass % relative to the phosphorescent metal complex (a).

The invention discloses a preparation method of blood coagulation factor VIII affinity chromatographic resin. The preparation method comprises the following steps: first weighing stored sepharose gel, washing the weighed sepharose gel, and activating the gel by adopting an epichlorohydrin activation method to obtain activated gel; performing an arm connection reaction for the obtained activated gel and spacing arm molecule N-hydroxysuccinimide to obtain arm-connected gel; performing a ligand coupling reaction for the arm-connected gel and peptide ligand; processing a coupling reaction product to obtain blood coagulation factor VIII affinity chromatographic resin; and washing the resin, and storing the resin in a 20 percent ethanol solution with three times volume of the gel under the condition of 4 DEG C. The product chromatographic resin is used for extracting blood coagulation factor VIII from blood plasma and can rapidly, specifically and efficiently purify the blood coagulation factor VIII.

The present invention is directed toward a multivalent product including a nanoparticle with a metal, metal alloy, or metal oxide core, a plurality of non-polymerizing ligands bound to the nanoparticle, and a plurality of paramagnetic ions coupled to the nanoparticle by the ligands. Methods of making and using the multivalent product are also disclosed.

The invention belongs to the technical field of nano-magnetic materials for biology, and relates to a nano-magnetic particle, in particular to an immune nano-cellulose magnetic liposome and a preparation method thereof. It is a core-shell structure, the core is magnetic material Fe3O4, the middle layer is a mixture of liposome and nanocellulose, and the outermost layer is the antibody coated on the middle layer; the mass content of nanocellulose is 0.1%~ 50.0%, the mass content of liposome components is 0.5% ~ 90.0%, and the mass content of magnetic materials is 0.1% ~ 50.0%. The immune nanocellulose magnetic liposome of the present invention has good biocompatibility. The ligand coupling in the substance can recognize and bind to the corresponding antigen, antibody or nucleic acid, etc., and can be separated and enriched rapidly in an external magnetic field. It can be used for the detection and separation and purification of biological materials, and can be used in cell separation, immobilized enzymes, targeted drugs and Immunoassays and other biological fields have broad application prospects.

The invention relates to the technical field of preparation of purified packing, in particular to preparation and application of super tolerance metal chelating affinity packing. The preparation method comprises the steps of ligand coupling and metal ion chelating. The obtained metal chelating affinity packing (IMAC) chelates quite firm metal ions, and can be applied to capturing and purifying protein with His-tag. The tolerance packing of the type can efficiently purify target protein on the condition that EDTA and DTT are contained, and the tedious treatment of switching buffer liquid before purification is avoided; in the using process, few metal ions are disengaged, 1M sodium hydroxide in-place cleaning can be carried out without stripping of metal ions, the regeneration treatment step for packing can be effectively simplified, and the packing has strong component compatibility, is applicable to buffer conditions of wide substrate and salinity, has the advantages of being high in capacity, high in purity, long in service life and low in using cost, and is suitable for being further applied and popularized.