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Stable nano gel with core-shell structure, preparation method and application thereof

A technology of nano-gel and shell structure, which is applied in the fields of food chemistry, pharmaceutical preparations, and polymer chemistry. It can solve the problems of cumbersome preparation process, large carrier particle size, polymerization, spray drying, and solvent evaporation. The method is simple and easy to achieve. , the effect of simple method

Inactive Publication Date: 2007-10-24
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drug carriers formed by biomacromolecules also have the following disadvantages: 1. The methods of preparation are usually emulsion polymerization, spray drying, solvent evaporation, etc., and organic solvents or emulsifiers or crosslinking agents are unavoidably used
2. The particle size of the carrier is too large, close to the micron scale
3. The natural polymer materials currently used are mainly concentrated in polyelectrolytes. The prepared drug carrier can enrich the drug, but it limits its stability in a relatively wide pH range and physiological ionic strength; Usually such polyelectrolyte nanomaterials need further surface modification, such as chitosan further combined with PEG {Journal of Applied Polymer Science, 1997, 63, 125-132}, modified PEG on albumin {International Journal of Pharmaceutics, 1999, 189, 161 -170} to improve the surface properties of nanospheres, the precursor preparation process is cumbersome
But so far, there has been no report on the preparation of globulin-polysaccharide covalent complexes by Maillard reaction and further preparation of protein-polysaccharide nanogels that are stable in the entire pH range.

Method used

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  • Stable nano gel with core-shell structure, preparation method and application thereof
  • Stable nano gel with core-shell structure, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Egg white lysozyme (Lysozyme) and dextran form nanogels.

[0021] Dissolve egg white lysozyme in deionized water to prepare a 10 mg / mL protein solution. Add dextran with a molecular weight of 10k, 35k, and 62k to the above solution to prepare a mixed solution of polysaccharides and proteins with different molecular weights, and make the molar ratio of dextran:albumin 1:10 to 10:1, and magnetically stir The solution was mixed, the pH of the solution was adjusted to 8, and the solution was freeze-dried. The freeze-dried solid was placed in an airtight container (relative humidity: 79%) filled with saturated KBr solution, and placed at 60° C. for Maillard reaction for 18-24 hours. The Maillard reaction product was dissolved in deionized water to prepare a covalent complex solution with a lysozyme concentration of 1 mg / mL. Adjust the pH value of the lysozyme-dextran covalent complex solution to 10.7 with 0.1mol / L NaOH, stir the solution for 15 minutes, then hea...

Embodiment 2

[0025] Example 2. Bovine Serum Albumin (Bovine Serum Albumin) and dextran form a nanogel.

[0026] Bovine serum albumin and dextran were subjected to Maillard reaction to synthesize bovine serum albumin-dextran covalent complex, and the Maillard reaction conditions were the same as Example 1. Dissolve the Maillard reaction product in deionized water to obtain a bovine serum albumin solution with a concentration of 1.0 mg / mL, adjust the pH value of the above solution to 5.2 with 0.1 mol / L HCl, and stir for several minutes, then place the solution at 80°C The nanogel solution of bovine serum albumin-dextran can be obtained by heating in a water bath for 1 hour. Table 2 shows the light scattering analysis results of the nanogels obtained under the above conditions with different molecular weight dextran and different molar ratios of dextran and bovine serum albumin.

[0027]

Embodiment 3

[0028] Example 3. Ovalbumin and dextran form nanogels.

[0029] Ovalbumin (Ovalbumin) and dextran were subjected to Maillard reaction to form ovalbumin (Ovalbumin)-dextran covalent complex, and the Maillard reaction conditions were the same as Example 1. Dissolve the Maillard reaction product in deionized water to obtain a solution with an ovalbumin concentration of 1.0 mg / mL, adjust the pH value of the above solution to 4.6 with 0.1 mol / L HCl, stir for several minutes, and then place the solution in a water bath at 80°C A stable nanogel solution can be obtained by heating in medium for 0.5 hour. The obtained nanogel solution was measured by dynamic laser light scattering: the average particle size was 112 nm, and the polydispersity coefficient was 0.24.

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Abstract

The invention discloses a stable nanometer gel of core-shell structure and application in the macromolecular chemistry and drug agent technical domain, which is characterized by the following: reacting protein and Maillard of polysaccharide to form protein-polysaccharide covalent compound; heating the protein-polysaccharide covalent compound solution under certain pH value; obtaining the product as micromolecular carrier; loading micromolecular drug and nourishing material in the dispersing or gel forming course; obtaining the nanometer particle of drug and nourishing material.

Description

technical field [0001] The invention relates to a nano gel with polysaccharide as the shell and protein as the core and its method and application, belonging to the technical fields of polymer chemistry, food chemistry and pharmaceutical preparations. Background technique [0002] Nanotechnology and biodegradable and biocompatible materials are current research hotspots in drug delivery systems {Journal of Controlled Release, 2001, 70, 1-20}. The use of nanoparticles as drug carriers can achieve the purpose of targeted delivery and sustained-release drug delivery. This is because nanoparticles can enter many human organ tissues that are difficult for large particles to enter, and can cross many biological barriers to reach lesion sites and tumor tissues. The drug can be delivered to the brain through the blood-brain barrier, and the drug can be enriched in the lymph nodes through oral administration {Polymer Bulletin, 2002, 3, 24-32}. [0003] At present, the research on na...

Claims

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Application Information

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IPC IPC(8): C08L5/00C08L89/00C08J3/00B32B9/00A61K47/36A23L1/0562A23L1/052A23L29/206A23L29/281
Inventor 姚萍李娟喻绍勇江明
Owner FUDAN UNIV
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