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Preparation method for chitosan-based porous scaffolds with biological activity

A bioactive, porous scaffold technology, applied in medical science, prosthesis, etc., can solve the problems of large influence on scaffold stability, reduce scaffold porosity, interfere with hydrogen bonding, etc., to improve biocompatibility, good pore size The effect of wall roughness and uncomplicated process

Inactive Publication Date: 2012-01-18
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although this simple compound can improve the biological activity of dry chitosan composites and improve the mechanical properties, the addition of inorganic materials interferes with the hydrogen bonding between chitosan molecules and reduces the crystallinity, thereby reducing its Stability in the aqueous phase for faster degradation
For this reason, some researchers (Jiang L Y, et al. Preparation and properties of nano-hydroxyapatite / chitosan / carboxymethyl cellulose composite scaffold [J]. Carbohyd Polym, 2008, 74: 680) added a certain amount of polyanion to ion exchange The cross-linking method obtains stable composite materials, but this method reduces the porosity of the scaffold, and the stability of the scaffold obtained by the cross-linking method is greatly affected by pH

Method used

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  • Preparation method for chitosan-based porous scaffolds with biological activity
  • Preparation method for chitosan-based porous scaffolds with biological activity
  • Preparation method for chitosan-based porous scaffolds with biological activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Preparation of chitosan / nano-hydroxyapatite composite porous scaffold

[0045] 1) Synthesis of water-soluble formylated carboxymethyl cellulose

[0046] First the water-soluble polysaccharide polymer sodium carboxymethyl cellulose is dissolved in water to form a mass percentage concentration of 5% polysaccharide polymer solution, and then the pH value of the polysaccharide polymer solution is adjusted to 3 with the sulfuric acid of 5mol / L in the dark Add sodium periodate 5 times the mass of the polysaccharide polymer in batches under the same conditions, react at 45°C for 6 hours under stirring, then add excess ethylene glycol to continue the reaction for 30 minutes; The ethanol mixture is fully washed until no iodine is detected; finally, water-soluble formylated carboxymethyl cellulose is obtained by vacuum drying at room temperature;

[0047] 2) Preparation of chitosan-hydroxyapatite slurry

[0048] Under stirring conditions, dissolve chitosan with a dea...

Embodiment 2

[0057] Example 2: Preparation of chitosan / β-tricalcium phosphate composite porous scaffold

[0058] 1) Synthesis of water-soluble aldylated polysaccharide hyaluronic acid

[0059] First, the water-soluble polysaccharide polymer hyaluronic acid is dissolved in water to form a polysaccharide polymer solution with a mass percent concentration of 2%, and then the pH value of the polysaccharide polymer solution is adjusted to 4 with 5mol / L sulfuric acid. Add sodium periodate 3 times the mass of the polysaccharide polymer in batches, react at 60°C for 4 hours with stirring, and then add excess ethylene glycol to continue the reaction for 30 minutes; Wash until no iodine is detected; finally, vacuum-dry at room temperature to obtain the water-soluble formylated polysaccharide hyaluronic acid;

[0060] 2) Preparation of chitosan-β-tricalcium phosphate slurry

[0061] Under stirring conditions, dissolve chitosan with a deacetylation degree of 90% and a molecular weight of 300,000 in ...

Embodiment 3

[0070] Example 3: Preparation of chitosan / α-tricalcium phosphate composite porous scaffold

[0071] 1) Synthesis of water-soluble aldylated polysaccharide alginic acid

[0072] First, the water-soluble polysaccharide polymer sodium alginate was dissolved in water to form a polysaccharide polymer solution with a mass percent concentration of 6%, and then the pH value of the polysaccharide polymer solution was adjusted to 1 with 5mol / L sulfuric acid, and then separated under light-proof conditions. Add sodium periodate 4 times the mass of the polysaccharide polymer in batches, react at 50°C for 5 hours with stirring, and then add excess ethylene glycol to continue the reaction for 30 minutes; Washing until no iodine is detected; finally, vacuum drying at room temperature to obtain the water-soluble aldylated polysaccharide alginic acid;

[0073] 2) Preparation of chitosan-α-tricalcium phosphate slurry

[0074] Under stirring conditions, dissolve chitosan with a deacetylation d...

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Abstract

The present invention discloses a preparation method for a chitosan-based porous scaffold with biological activity. According to the present invention, chitosan and inorganic micro / nanoparticles with biological activities are adopted as raw materials to prepare into slurry; a water-soluble aldehyde polysaccharide polymer is adopted as a cross-linking agent; a Schiff base cross-linking process and a freeze drying method are adopted to prepare a class of chitosan-based composite porous scaffold materials with biological activities, wherein porosities, pore sizes, mechanical properties, degradation performances, and bone conductions / bone inductions and the like can be regulated through the formula and the process conditions. According to the present invention, the porous scaffold provided by the present invention does not adopt harmful chemical cross-linking agents, and can not cause adverse effects to cells; with the cross-linked porous structure and a plurality of hydrophilic groups such as amino, carboxyl, hydroxyl and the like, the porous scaffold has high chemical stability in an aqueous phase with a wide pH range (pH range of 5-9), and has outstanding advantages of softness, high elasticity, swelling ratio of 30 times, and the like; the porous scaffold has advantages of imitation of natural bone extracellular microenvironment in aspects of the porous structure, the chemical composition, the surface chemistry and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials in regenerative medicine technology, and relates to a tissue engineering porous scaffold and a preparation method, in particular to a preparation method of a bioactive chitosan-based porous scaffold suitable for hard tissue repair such as bone. Background technique [0002] Tissue engineering technology is the most promising new method of defect tissue regeneration and repair developed in recent years, and has become the most active research direction in the field of regenerative medicine. Its essence is to provide a highly simulated three-dimensional environment for seed cells by constructing a microenvironment in vitro that simulates the extracellular matrix in natural tissues, that is, a porous scaffold, including spatially porous structure, matrix chemical composition and composition, and bioactive factors. In order to facilitate cell adhesion, differentiation, and proliferation, tissues wi...

Claims

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Application Information

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IPC IPC(8): C08J9/36C08J9/28C08J3/24C08L5/08C08L1/26C08L5/00C08L5/04C08K3/32C08K3/40C08B15/00C08B37/08C08B37/04C08B37/00A61L27/44A61L27/46A61L27/56
Inventor 钱军民徐明辉金欣霞杨腾飞
Owner XI AN JIAOTONG UNIV
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