67 results about "Synthetic antigen" patented technology

The present invention discloses an affinity-binding assay comprising a particle having at least four copies of a target molecule and at least two binding molecules specific for the target molecule, wherein a first of the binding molecules is associated with a first label and a second of the binding molecules is associated with a second label, wherein a particle having the first label and a particle having the second label are distinguishable from a particle having both the first and second labels, wherein the first and second binding molecules each comprise at least two binding regions specific for the target molecule. The present invention also discloses a composition comprising a first binding molecule associated with a first label and a second binding molecule associated with a second label, wherein the signal obtained from the first and second labels is distinguishable from the combined signal of the first and second labels, characterized in that the first and second binding molecules each comprise at least two binding regions specific for essentially the same target molecule, preferably for essentially the same epitope on the target molecule and a method for selecting a synthetic antigen from a collection of at least three antigens comprising using the disclosed composition and method. The invention further discloses an antigen obtainable by the above-described method and capable of inducing an early immune response, a kit of parts to perform the method, the use of antigens selected by the methods for use as a vaccine, and the use of antibodies and antigens as a medicament.

The present invention relates to an antibody, recombinant or synthetic antigen-binding fragments thereof able to recognise and bind an epitope comprised in the TrkA amino acid sequence, medical uses thereof and a pharmaceutical composition comprising at least one of the above antibody, recombinant or synthetic antigen-binding fragments thereof.

The invention discloses an enzyme-linked immunoassay kit of a structural protein antibody for a seneca valley virus. The kit comprises an elisa plate, positive control serum, negative control serum, an HRP-conjugated antibody, a sample diluent, a 20-fold concentrated detergent, a substrate solution A, a substrate solution B and a stop solution, wherein the elisa plate is coated with a structural protein epitope polypeptide composition for the seneca valley virus. The epitope polypeptide composition is one or any combination of more than two of a polypeptide as shown in a sequence 1, a polypeptide as shown in a sequence 2, a polypeptide as shown in a sequence 3 or a polypeptide as shown in a sequence 4 in the sequence table. The elisa plate is coated with a chemical synthetic antigen peptide, so that the kit is low in antigen dosage and high in sensitivity and specificity, and whether the structural protein antibody is infected by the seneca valley virus or not can be efficiently detected. The kit is high in sensitivity, good in specificity, convenient in operation, and has a good market prospect.

The invention provides a vitamin D synthetic antigen and a preparation method thereof. The vitamin D synthetic antigen is a conjugate of vitamin D and a protein carrier. The vitamin D is 25-hydroxy vitamin D3 or 1,25-dihydroxy vitamin D3; and the protein carrier is one or more selected from bovine serum albumin, ovalbumin, hemocyanin and human serum albumin. The invention also provides application of the vitamin D synthetic antigen to vitamin D immunological detection. The invention further provides a vitamin D detection kit, which integrates the advantages of existing clinical vitamin D detection methods; and the kit can be applied to all enzyme mark instruments, chemiluminescence instruments and time-resolved analyzers, and has greatly shortened detection time, and sensitivity, accuracy and precision met the detection requirements. The immunosorbent assay kit with strong versatility provided by the invention can realize batch and rapid detection on vitamin D in serum (or plasma).

The invention discloses a dendrite-shaped cell vaccine for loading a recombination human heat shock protein 70 polypeptide composite, a method for preparing the same and application thereof. The preparation method comprises: preparing a dendrite-shaped cell by designing and synthesizing an antigen polypeptide, forming the composition by the antigen polypeptide and the recombination human heat shock protein 70(rhHSP70), and loading the composite on the dendritic cell (DC) to obtain the vaccine. The vaccine has the advantages of multi-target points, double adjuvants and high efficacy, and can be used for resisting tumors and treating infectious diseases. The preparation process is simple and fast, and low in cost.

The invention discloses an anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. According to the preparation, 10-70 parts by weight of porphyromonas gingivalis thalli and thalli fragments, and 30-90 parts by weight of fusobacterium nucleatum thalli and thalli fragments are compounded into an antigen to immune an egg laying hen. The invention also discloses the specific preparation method of the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation, and toothpaste containing the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. By the IgY toothpaste which is used for treating and preventing gingivitis and ozostomia has obvious effects in preventing and treating the gingivitis and the ozostomia.

The present invention relates to synthetic antigen-presenting matrices, their methods of making and their methods of use. One such matrix is cells that have been transfected to produce MHC antigen-presenting molecules and assisting molecules such as co-stimulatory molecules. The matrices can be used to activate CD8+ T-cells to produce cytokines and become cytotoxic.

The present invention relates to an antibody, recombinant or synthetic antigen-binding fragments thereof able to recognize and bind an epitope comprised in the spacer domain of ADAMTS-5, nucleic acid and expression vector encoding the same, method of production and uses thereof.

This invention is in the field of recombinant Plasmodium falciparum polypeptides and relates to recombinant or synthetic antigen compositions which comprise p42 antigens, and more specifically to methods and compositions for the expression of Plasmodium falciparum polypeptides in transgenic plants.

The invention provides a method of detecting small molecule substances based on chemiluminescence immunology of golden-magnetic particles. The method mainly comprises the following steps: (1) coating: a step of using a golden-magnetic particle as carriers for immunoreaction and solid separation, using a small molecule substance corresponding to a small molecule substance to be detected to synthesize an antigen and coupling the antigen onto the surface of the golden-magnetic particle; (2) enclosing: a step of enclosing blank sites on the surface of the golden-magnetic particle which have not bound to the antigen by using blocking buffer , carrying out magnetic separation and discarding supernatant; (3) reaction with the substance to be detected: a step of adding a sample to be detected, an antibody against the small molecule substance to be detected and enzyme labeled secondary antibody capable of specifically binding to the antibody into the golden-magnetic particle which has bonded with the antigen synthesized from the small molecule substance so as to form a specific antigen-antibody-enzyme labeled secondary antibody compound; (4) cleaning and (5) detection. The detection method provided in the invention has the advantages of high sensitivity, good specificity, a wide linear range, high precision, good stability, no radioactive pollution, safe operation, simpleness and rapidity.

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.

A gold-labeling chromatography method for quickly detecting beta-excitant includes reaction of liquid speciment with gold-labelled clenbuterol antibody to obtain antigen-antibody compound, competing with clenbuterol-BSA coupled substance for the compound antibody, and reaction of said antigen-antibody compound or gold labeling antibody with the antibody. Its advantage is high speed.

The invention relates to a detection technology for detecting paralytic shellfish poisoning, in particular to a method for preparing an anti-paralytic shellfish poisoning monoclonal antibody by using protein coupling to synthesize an antigen, and relates to the use of monoclonal antibody and gonylin GTX2 / 3 antigen Combination, and the preparation method of the quick detection kit that analyzes the content of gonylin GTX2 / 3 in the biological sample. An enzyme-linked immunoassay kit for rapid detection of the main component of paralytic shellfish poisoning, gonyellin GTX2 / 3, including a 96-well microtiter plate with detachable strips, enzyme-labeled secondary antibody, and buffer salt wash containing Tween-20 solution, substrate solution and stop solution, using the prepared monoclonal antibody containing specific anti-gonitoxin GTX2 / 3, on the 96-well ELISA plate coated with the coated antigen of the synthetic protein conjugate. The kit of the invention has the advantages of simple pretreatment, easy operation, and is suitable for on-site, fast, and large-volume sample detection. The kit has low cost and low price, and the detection limit of the kit is less than 15ng / ml.

The invention discloses a comprehensive detection method for an allergen in a TCM injection, and relates to the field of immunology and science of TCM. According to the invention, the method is based on combined ELISA (enzyme linked immunosorbent assay) principles and fingerprint chromatogram variance analysis, uses an immunoreaction of embedding antigens, IgE antibodies or anti-IgE antibodies with solid carriers and respectively carries out labeled immunoreaction between TCM injection component antigens and labeled anti-IgE antibodies, the embedded IgE antibodies and labeled antigens, the embedded IgE antibodies and known synthetic antigens, the embedded IgE antibodies and enzyme labeled antibodies, the embedded IgE antibodies and IgE antibodies in a blood sample captured by the embedded IgE antibodies, the labeled antigens or antigens and labeled antibodies; the captured IgE antibodies are combined with TCM injection components, and fingerprint chromatogram variance analysis is carried out by using GC / MS and HPLC / MS; specific IgE and corresponding allergens in human and animal blood samples allergic to the TCM injection are determined based on comprehensive consideration of information of a plurality of aspects. The detection method for the TCM injection provided in the invention has the characteristics of high specificity, high sensitivity, high efficiency and easy and convenient operation, is applicable to simultaneous detection of a plurality of components and can effectively prevent omission.

The invention discloses an avian influenza virus H9 subtype antibody enzyme-linked immunoassay kit. The kit comprises an enzyme-labelled plate, a positive control serum, a negative control serum, an enzyme-labelled second antibody, a sample diluent, a sample diluent, a 20-fold concentrated washing solution, a substrate solution A, a substrate solution B and a stop solution, wherein the enzyme-labelled plate coats an avian influenza virus H9 subtype HA protein epitope polypeptide composition. The epitope polypeptide composition is any combination of one or more than two of polypeptide represented by a sequence 1 in a sequence list, polypeptide represented by a sequence 2 in the sequence list or polypeptide represented by a sequence 3 in the sequence list. The avian influenza virus H9 subtype antibody enzyme-linked immunoassay kit in the invention adopts an indirect method ELISA and uses a chemical synthetic antigen peptide to coat the enzyme-labelled plate; the use amount of antigen islow, and the sensitivity and the specificity are high; it can be effectively detected whether the avian influenza virus H9 subtype antibody exits. The kit in the invention has advantages of high sensitivity, good specificity, convenience for operations and good market aspect.

The invention discloses a drug trace detection test strip, a preparation method thereof and a drug trace detection kit. The drug trace detection test strip comprises a test strip body, wherein the test strip body comprises a substrate, and a sample pad, a combination pad, a detection film and an absorption pad which are arranged on the substrate and are sequentially connected from one end to the other end of the substrate; the combination pad is provided with at least one time-resolved fluorescent microsphere-labeled specific monoclonal antibody of drugs; the detection film is provided with atleast one detection line corresponding to each drug; each detection line is coated with a synthetic antigen of the corresponding drug; and the six drugs are one or more of morphine, phenylalanine, ketamine, tetrahydrocannabinol, cannabis synthetic hormone and cocaine. The drug trace detection test strip has the advantages of high detection speed, low cost and simple operation.

The present invention relates to an antibody, recombinant or synthetic antigen-binding fragments thereof able to recognise and bind an epitope comprised in the TrkA amino acid sequence, medical uses thereof and a pharmaceutical composition comprising at least one of the above antibody, recombinant or synthetic antigen-binding fragments thereof.

Compositions and methods for the detection of Taenia solium and the diagnosis of T. solium infection are described. The nucleotide and amino acid sequences of the antigenic T. solium polypeptides gp50a, gp50b and gp50c are provided. The compositions contain synthetic antigenic polypeptides of larval origin prepared using the sequences described herein. Probes and primers for the detection or amplification of T. solium nucleic acid molecules are also described. The polypeptides can be administered to a human or animal to protect against T. solium infection. In addition, the polypeptides are useful as research tools for studying T. solium and as reagents in assays for the detection of T. solium antibodies in a biological sample. The methods are sensitive and specific assays that utilize the stable recombinant or synthetic antigenic polypeptides or nucleic acid molecules encoding the larval polypeptides.

The invention provides a method for intensifying functions of cytokine-induced killer cells. The method comprises the following steps: analyzing and obtaining an epitope through a bioinformatics analysis method, fusing the epitope and Ii-Kye into an epitope peptide, anchoring the epitope peptide on MHC-II molecules on the surfaces of dendritic cells, and infecting the dendritic cells with recombinant adenovirus rAd-RNAi-SOCS1; and further carrying out co-cultivation on the dendritic cells and cytokine-induced killer cells, and treating tumor-bearing mice with co-cultivated cells, so that the processed dendritic cells are capable of activating the cytokine-induced killer cells, and intensifying the significant killing functions of the cytokine-induced killer cells on related tumors. The invention provides a method for treating tumors employing activated cells; and the core technology of the method has reference significance on treatment of other viroses.

The invention discloses a flavanone-3-hydroxylase antigen epitope peptide, a fusion protein and a specific antibody of the flavanone-3-hydroxylase antigen epitope peptide, and a preparation method andapplication of the antibody. The amino acid sequence of the flavanone-3-hydroxylase antigen epitope peptide is as shown as SEQ ID NO:1. After being modified, the antigen epitope peptide is subjectedto solid-phase synthesis and is coupled with a carrier protein to obtain the fusion protein used as an artificially synthetic antigen, and the amino acid sequence is as shown as SEQ ID NO:2. The specific antibody of the invention is obtained by immunizing animals by the artificially synthetic antigen and performing separation and purification. The prepared polyclonal antibody has the advantages ofhigh affinity, high specificity, high sensitivity, operation simplicity and convenience, and can perform specific binding reaction with rice flavanone-3-hydroxylase; in addition, the preparation costis low; the yield is high; and an important tool is provided for the relevant physiological function study and identification of the rice flavanone 3-hydroxylase.

The invention discloses a hog cholera virus E2 protein epitope and preparation and application of an antibody. The sequence of the epitope is as shown in SEQ ID NO.1, and the epitope is positioned at105-109AA. The epitope can be reacted with CSFVs (Classical Swine Fever Viruses), but does not react with PK-15 cells. The epitope is applied to hog cholera virus antibody medicine detection agents. Due to the application, a hog cholera virus antibody diagnosis antigen can be prepared by using a common chemical synthesis method, and compared with a genetic engineering expression protein as a diagnosis antigen, the synthesized antigen is simple and convenient in process and relatively high in purity.

A method of eliciting immune responses using synthetic antigens involves generating a substitute antigen configured for a foreign molecule, generating a synthetic high affinity ligand molecule (SHAL) comprising at least one ligand configured to bind to an antigen presenting cell (APC) and at least one ligand specifically configured to bind with the substitute antigen, combining the SHAL with the substitute antigen through a chemical reaction forming an antigen presenting complex, introducing the antigen presenting complex to a user without an immune response to the foreign molecule.

The invention relates to a latex microsphere immunodetection test strip, a kit and a method. The latex microsphere immunodetection test strip comprises a sample pad, a combination pad, a detection membrane, an absorption pad and a bottom plate, wherein the sample pad, the combination pad, the detection membrane and the absorption pad are sequentially connected from one end to the other end of the bottom plate; the combination pad is coated with a specific monoclonal antibody marked by latex microspheres, the specific monoclonal antibody marked by the latex microspheres can react with drugs, and the drugs comprise phencyclidine and oxycodone; a detection line and a quality control line formed by quality control protein are arranged on the detection film, and the detection line is coated with synthetic antigens of corresponding drugs. The latex microsphere immunodetection test strip can rapidly detect oxycodone and phencyclidine in hair, is high in sensitivity and good in specificity, does not need a detection instrument, and is convenient to operate.

The invention relates to a drug detection test strip and a drug detection kit. The drug detection test strip comprises a sample pad, a combination pad, a detection film, an absorption pad and a bottom plate, wherein the sample pad, the combination pad, the detection film and the absorption pad are sequentially connected on the bottom plate; the combination pad is provided with a specific monoclonal antibody marked by luminous microspheres of at least one drug, the detection film is provided with at least one detection line corresponding to the drug, the detection line is coated with a synthetic antigen corresponding to the drug, and the drug comprises at least one or a composition of hemp, an echinoka and cocaine. The drug detection test strip can detect three common drugs in hair at the same time, is convenient to take materials, stable to store, high in sensitivity and good in specificity, can effectively improve the detection efficiency of law enforcement officers, and meanwhile, reduces the detection cost.

Novel polypeptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.

The present invention relates to an antibody, recombinant or synthetic antigen-binding fragments thereof able to recognize and bind an epitope comprised in the TrkA amino acid sequence, medical uses thereof and a pharmaceutical composition comprising at least one of the above antibody, recombinant or synthetic antigen-binding fragments thereof.

The present invention relates to synthetic antigen-presenting matrices, their methods of making and their methods of use. One such matrix is cells that have been transfected to produce MHC antigen-presenting molecules with one or more accessory molecules. The matrices are used to activate naive CD4+ T cells as well as shift the ongoing activation state into a preferred differentiated population of either Th1 or Th2 cells.

The invention relates to a process for preparing a 2, 4 drop synthetic antigen with an increased partition arm, and belongs to micro-molecular environmental pollutant immune detection field. The process includes two methods: The first is synthesizing condensate of the 2, 4 drop and a molecule with a C3 chain or a C6 chain and cross linking it with a carrier protein. The second is linking molecules with C3 chains or C6 chains on surface of the carrier protein and condensing carboxy with amino acting as a partition arm molecule of the of 2, 4 drop. The invention can acquire the 2, 4 drop antibody with strong specificity and good affinity.