29 results about "Synthetic antigen" patented technology

Provided herein are methods for preparing and characterizing SARS-co2 antigen specific immune cell cultures and preparations and methods of using the same in adoptive immunotherapy for cancer, infections and immune disorders. Also, provided are compositions and methods for generating immune cells expressing synthetic antigen binding receptors targeting SARS-cov2 and methods of use of these cells for the treatment and prevention of COVID-19. Also provided are compositions and methods for determining immune response to SARs-cov2 in a subject, detecting SARS-cov2, measuring cytotoxicity induced by SARS-cov2, and detecting the expression and cytotoxicity of synthetic antigen binding receptors targeting SARS-cov2.

The invention relates to the technical field of vaccines, and discloses a preparation method and application of an anti-tumor vaccine based on an autoantigen and a synthetic antigen. The anti-tumor vaccine comprises a tumor autoantigen and a synthetic antigen, wherein the tumor autoantigen is extracted from a tumor cell membrane, a tumor cell lysate, a tumor cell secretion, an exosome antigen or an antigen released by a tumor microenvironment; the synthetic antigen is selected from at least one of a tumor associated antigen, a tumor specific antigen, a personalized neoantigen, a pathogen antigen, a biotoxin, a biomolecule antigen and coding nucleic acid of a peptide type antigen in the antigens; the coding nucleic acid is DNA or mRNA. The vaccine molecule composed of the tumor autoantigen and the synthetic antigen is easy to prepare, has a strong anti-tumor immune effect and good biological safety, solves the problems that the traditional tumor autoantigen has no specific precise target spot and the synthetic antigen is single in immunogen, and has a wide application prospect.

The present disclosure provides feeder hydrogel particles that can function to support the growth, proliferation, and / or activation of a target cell in culture. The present disclosure also provides methods of culturing target cells with feeder hydrogel particles.

Nanoparticles for engineering cells to express target antigens and methods of treating / preventing cancer or solid tumors using the same.

The invention belongs to the technical field of biological medicine and disease prevention and treatment, and particularly relates to an SSEA4 conjugated virus-like particle anti-cancer vaccine as well as a preparation method and application thereof. Specifically, according to the vaccine, an SSEA4 antigen is efficiently synthesized through a chemical enzyme method, then the SSEA4 antigen is covalently coupled with a virus-like particle (VLP), the SSEA4 antigen and the VLP are combined with a monophosphoryl lipid A adjuvant with high safety, an organism can be induced to generate strong and lasting SSEA4-resistant humoral immune response, the generated antibody can specifically recognize and kill SSEA4 positive tumor cells, and the SSEA4 positive tumor cells can be effectively killed. Meanwhile, good safety is shown in an animal model, a new solution is provided for immunotherapy of SSEA4 positive tumors, and therefore the SSEA4 positive tumor immunotherapy polypeptide has wide application prospects and market value.

The invention relates to a method for the generation of a TCR in a rodent, method comprising delivering an antigen presenting cell (APC) expressing an MHC-peptide complex to the rodent, wherein the MHC component of the MHC peptide is not expressed and generating T-cells to the MHC-peptide complex in the rodent. The method further comprises isolating or purifying the T-cell from the rodent. The MHC component is either allogenic or xenogenic and the MHC expressed in the rodent is a human HLA, such as HLA-A*02:01, HLA-A*11:01, HLA-A*03:01 or HLA-A*24:02. The rodent genome encodes a fully human TCR. The peptide is from a human antigen associated with disease, such as a tumour associated antigen. The rodent genome comprises human CD8 or chimeric CD8. The TCR sequence has been modified by up to 3 amino acids from that identified in the rodent. Also disclosed is a method for generating a TCR in a mouse, identifying the nucleic acid encoding the TCR and then mutating the sequence. The TCR is expressed in the form of a larger molecule. The APC is a synthetic antigen presenting cell.

The invention discloses a method for stereospecifically synthesizing a Tn antigen and a TF antigen, and belongs to the technical field of synthesis of the Tn antigen and the TF antigen, and the specific method comprises the following steps: taking 2-azido-2-deoxy-3-O-tertiary butyl dimethyl silyl-4, 6-di-O-benzoyl-galactosyl phenyl thioester as a donor, taking serine as a receptor, and carrying out a one-pot reaction to obtain the Tn antigen and the TF antigen. Adopting a remote adjacent group to participate in effect stereospecificity to synthesize a protected Tn antigen; selectively removing tert-butyldimethylsilyl of the Tn antigen to dissociate 3-site hydroxyl, and reacting with peracetylated galactosyl trichloroacetimidate to obtain a protected TF antigen; and finally, removing protecting groups for protecting the Tn antigen and the TF antigen to obtain the Tn antigen and the TF antigen. The synthesis method has the advantages of mild conditions, low cost, high yield and the like, and meanwhile, the remote participation effect can be enhanced, and the stereospecificity can be efficiently realized.

The application provides novel peptide epitope tags and recombinant polynucleotide, polypeptides, vectors, cells and compositions comprising the tags. The application also provides novel designs for synthetic antigen receptors (SARs), novel antigen binding domains, novel SAR constructs and novel methods for manufacturing of cell therapy products. These novel methods and compositions have broad uses in cellular therapy.

Provided herein is an immunogenic composition comprising a synthetic antigen to COVID spike proteins, particularly the chimeric intermediate structure C-A Complex. Also disclosed herein is a method of preventing and / or treating a COVID infection in a subject in need thereof, by administering the immunogenic composition to the subject.

The present disclosure provides feeder hydrogel particles that can function to support the growth, proliferation, and / or activation of a target cell in culture. The present disclosure also provides methods of culturing target cells with feeder hydrogel particles.

The present inventors have identified a combination vaccine for immunization against bacterial meningitis caused by multiple pathogens.

The present invention relates to the field of biological detection, and in particular to a colloidal gold test strip for detecting agricultural and veterinary drugs and illegal additives and a preparation method thereof. The test strip comprises a base plate and a sample pad, a colloidal gold binding pad, a buffer pad, a reaction membrane, a water-absorbing pad, and a self-adhesive sticker which are tightly connected in sequence on the base plate; the colloidal gold binding pad is coated with a labeled antibody that can bind to the agricultural and veterinary drugs and illegal additives; the buffer pad is coated with a buffer solution; an amino acid line, a detection line, and a quality control line are sequentially arranged along the chromatography direction on the reaction membrane; the amino acid line is coated with an amino acid solution; the detection line is coated with an artificially synthesized antigen, and the quality control line is coated with a sheep anti-mouse IgG secondary antibody that can bind to the labeled antibody. The test strip of the present invention can be quickly used for the detection of agricultural and veterinary drugs and illegal additives, with high sensitivity, good accuracy, high precision, and uniform color development, which effectively solves the problems of low sensitivity, uneven color development, and insufficient accuracy of existing products.

The invention relates to the technical field of antibodies, in particular to a monoclonal antibody generated by an aconitine hybridoma cell strain and application of the monoclonal antibody. The aconitine hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the aconitine hybridoma cell strain is classified and named as the aconitine hybridoma cell strain, the preservation date is June 5, 2025, and the preservation number is CGMCC No.46541. The preparation method comprises the following steps: preparing an artificially synthesized antigen with immunocompetence and characteristics by taking aconitine as a raw material, inducing a mouse to generate a specific antibody in an immunization manner, and then preparing a hybridoma cell strain capable of specifically recognizing diester-type aconitine (aconitine, hypaconitine and mesaconitine) through cell fusion. The generated monoclonal antibody is successfully applied to products such as an immunoaffinity column, an enzyme-linked immunosorbent assay kit and a colloidal gold immunochromatography detection card.

Helicobacter pylori is a leading cause of gastric mucosal inflammation, peptic ulcers, and gastric adenocarcinoma. Emerging antimicrobial-resistant H. pylori has hampered the successful eradication of frequent chronic infections. Additionally, due to the absence of effective vaccines against H. pylori, a safe vaccine is highly demanded. Disclosed herein are innovative Protective Immunity Enhanced Salmonella Vaccine (PIESV) vector strains to deliver and express multiple H. pylori antigen genes. Immunization of mice with a vaccine delivering the HpaA, NapA (also termed Hp-NAP), UreA and UreB antigens, provided sterile protection against H. pylori SS1 infection in 7 out of 10 tested mice. Compared to the control groups that had received PBS or a PIESV with an empty vector, immunized mice exhibited specific and significant cellular recall responses and antigen-specific IgG2c, IgG1, total IgG and gastric IgA antibody titers. Importantly, the mice immunized with the vaccine candidate showed a significant reduction in a load of an unidentified Gram-positive rod-shaped bacteria in their stomach compared to the control groups. In conclusion, a Salmonella Typhimurium-based live vaccine delivering four antigens shows promise as a safe and effective vaccine against H. pylori infection.

This application provides novel viral envelope glycoproteins for pseudotyping viral vectors, novel designs of synthetic antigen receptors (SARs), novel signaling chains for constructing SARs, novel antigen-binding domains, and novel methods for producing SAR-expressing cells. These novel methods and compositions are widely used in cell therapy.

The application provides novel peptide epitope tags and recombinant polynucleotide, polypeptides, vectors, cells and compositions comprising the tags. The application also provides novel designs for synthetic antigen receptors (SARs), novel antigen binding domains, novel SAR constructs and novel methods for manufacturing of cell therapy products. These novel methods and compositions have broad uses in cellular therapy.

The present invention provides protected tetrasaccharides, their process of preparation and their use in the synthesis of oligosaccharides, in particular fragments of O-antigens from Shigella flexneri, for example of serotype 1a, 1b, 2a, 2b, 3a, X, 4a, 4b, 5a, 5b, 7a or 7b.

Provided herein are methods for preparing and characterizing SARS-cov2 antigen specific immune cell cultures and preparations and methods of using the same in adoptive immunotherapy for cancer, infections, and immune disorders. Also provided are compositions and methods for generating immune calls expressing synthetic antigen binding receptors targeting SARS-cov2 and methods of use of these cells for the treatment and prevention of COVID-19. Also provided are compositions and methods for determining immune response to SARS-cov2 in a subject, detecting SARS-cov2, measuring cytotoxicity induced by SARS-cov2, and detecting the expression and cytotoxicity of synthetic antigen binding receptors targeting SARS-cov2.

Presented herein are techniques for computer simulation design of customized, engineered antigens. Specifically, in certain embodiments, the methods and systems of the present disclosure provide for engineering antigens to reduce its activation to memory immune responses, such as B-cell and / or T-cell based responses, when introduced into a subject. For example, antigens designed in this manner can improve their performance as vaccinating immunogenic compositions. For example, unwanted to be bound by any particular theory, it is believed, among other things, that a reduction in the degree of triggering a memory immune response may result in an increased production of new antibodies that are selectively tailored by the subject's immune system to neutralize particular (e.g., occurring) epitopes of reference antigens.

The invention discloses a fluorescent microsphere immunochromatography test strip for detecting aflatoxin B1, a preparation method and a detection method. The test strip comprises a bottom plate, and filter paper, a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper are sequentially arranged on the upper side face of the bottom plate in a lap joint mode; a core-shell structure nanoparticle-fluorescent microsphere labeled aflatoxin B1 antibody A is attached to the glass fiber membrane, the fluorescent microsphere serves as a core, and the aflatoxin B1 antibody A is attached to the outer side face of the fluorescent microsphere in a coupled mode to serve as a shell; a detection line coated with an aflatoxin B1 antibody B and a quality control line coated with a secondary antibody or an aflatoxin B1 synthetic antigen are arranged on the nitrocellulose membrane. According to the detection method disclosed by the invention, the concentration of the aflatoxin B1 in the sample test solution is calculated by analysis software of a fluorescence analyzer, so that the result data of the content of the aflatoxin B1 in the sample to be detected, which is obtained by conversion, is high in reliability, good in accuracy and good in stability.

The invention discloses a valproic acid detection kit and application thereof.The valproic acid detection kit comprises a reagent R1 and a reagent R2, the reagent R1 is a carboxyl magnetic bead reagent coated with a valproic acid antibody, and the concentration of carboxyl magnetic beads is 0.1-1 mg / mL; the R2 reagent is a valproic acid synthetic antigen-alkaline phosphatase marker reagent, and the protein concentration of the R2 reagent is 0.1-2 mg / ml. The valproic acid detection kit is composed of specific reagents, the storage stability is improved, non-specific binding is reduced, a detection value stably falls within an acceptable error range of a true value, the reliability of a detection result is improved, meanwhile, expensive instruments are not needed, the cost is reduced, and the detection kit is suitable for large-scale popularization and application. Meanwhile, valproic acid in a sample can be rapidly, simply, stably and accurately detected.

The disclosure provides single-chain and multi-chain synthetic antigen receptors, methods of making such synthetic antigen receptors and uses thereof for the treatment of diseases and disorder.

The present invention discloses tumor antigen peptides targeting CD73, vaccines, and preparation methods and applications thereof. Based on the amino acid sequence of CD73 protein, the present invention combines MHC typing to synthesize antigen peptides for targeted and immunotherapy of solid tumors, including drugs and vaccines for the preparation of new antigen-reactive T cell therapy and engineered T cell therapy. The tumor peptide vaccine targeting CD73 provided by the present invention has good immunogenicity and strong targeted killing ability of induced antigen-specific T cells.

The present invention discloses a process for preparing progastrin (PG80) antibody and establishing a chemiluminescence immunoassay (CLIA) kit for detecting PG80. A PG80-N antigen epitope peptide is synthesized, and the amino acid sequence is SEQ ID NO.1. A PG80-C antigen epitope peptide is synthesized, and the amino acid sequence is SEQ ID NO.2. A carrier protein is coupled to prepare a PG80-N carrier protein antigen and a PG80-C carrier protein antigen. Animals are immunized to produce PG80-N or PG80-C monoclonal antibodies or polyclonal antibodies, which are used to establish a CLIA kit for detecting PG80, detect the PG80 level in blood or other body fluids, and can be used for early screening of various cancers, determining cancer progression or recurrence, and evaluating cancer treatment effects.

The present application provides novel viral envelope glycoproteins for viral vector pseudotyping, novel designs for synthetic antigen receptors (SARs), novel signal chains for constructing SAR, novel antigen binding domains, and novel methods for generating SAR expressing cells. These novel methods and compositions have broad applications in cell therapy.

Presented herein are technologies directed to in-silico design of custom, engineered, antigens. In particular, in certain embodiments, methods and system of the present disclosure provide for engineering antigens to reduce their activation of a memory immune response, such as B cell and / or T cell based response, when introduced into a subject. Designing antigens in this manner, may, for example, improve their performance as immunogenic compositions for purposes of vaccination. For example, without wishing to be bound to any particular theory, it is believed that, among other things, reducing the extent to which a memory immune response is triggered can lead to improved production of new antibodies that are selectively tailored by the subject's immune system to neutralize particular (e.g., arisen) epitopes of a reference antigen.

The application provides novel peptide epitope tags and recombinant polynucleotide, polypeptides, vectors, cells and compositions comprising the tags. The application also provides novel designs for synthetic antigen receptors (SARs), novel antigen binding domains, novel SAR constructs and novel methods for manufacturing of cell therapy products. These novel methods and compositions have broad uses in cellular therapy.

The present description relates to synthetic antigen receptors for use with covalent immune recruiters (CIRs) comprising an donor moiety, a covalent binding group, and a target binding domain (TBD), the synthetic antigen receptor comprising an extracellular acceptor domain and an effector domain, wherein the extracellular acceptor domain is capable of binding to the donor moiety of the CIR. The present description also includes engineered cells comprising the synthetic antigen receptors, methods and uses thereof, for example, for recognition of target cells by immune cells comprising the synthetic antigen receptor functionalized with a CIR.

The invention belongs to the field of salicylic acid detection, and particularly relates to a salicylic acid chemiluminescence immunoassay kit as well as preparation and application thereof. The kit provided by the invention comprises an R1 reagent (1-3 [mu] m carboxyl magnetic bead coated salicylic acid antibody, a magnetic bead preservation buffer solution containing glycine and bovine serum albumin) and an R2 reagent (alkaline phosphatase coupled salicylic acid synthetic antigen, and an enzyme working solution containing mouse IgG and MgCl2), and by combining a flow injection technology, the detection sensitivity of the kit is improved. The problems that no special kit exists in the existing salicylic acid detection, the high performance liquid chromatography is high in cost, and the spectrophotometric method is poor in sensitivity are effectively solved. The kit provided by the invention can be stably stored for 1 year at 4 DEG C, the deviation between the detection result and the high performance liquid chromatography is less than + / -1%, 60 samples can be detected per hour, the cost is low, the operation is simple and convenient, and the kit is suitable for primary medical institutions and large sample detection.