Method for detecting protein and amino acid in rapeseeds

A detection method, rapeseed technology, applied in the field of detection of protein and amino acid, to achieve the effect of simple sample pretreatment

Inactive Publication Date: 2011-01-05
HUAZHONG AGRI UNIV
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  • Method for detecting protein and amino acid in rapeseeds
  • Method for detecting protein and amino acid in rapeseeds
  • Method for detecting protein and amino acid in rapeseeds

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[0032] The rapid detection method of protein and amino acid in rapeseed provided by the present invention is as follows: first obtain the rapeseed to be tested in a dry state, collect near-infrared spectrum, and then use a mathematical model to convert the collected near-infrared spectrum into the quality of rapeseed to be tested Parameters, the quality parameters are one or more of crude protein and amino acid content, the mathematical model is established by partial least squares method, and the modeling samples used come from the National Rapeseed Engineering Technology Research Center of Huazhong Agricultural University.

[0033] The present invention will be further described below by taking the modeling method of rapeseed protein content as an example.

[0034] A rapid detection method for protein and amino acid in rapeseed, the steps are:

[0035] A. Put the harvested rapeseed in an oven to dry, control the humidity at 103 or 104 or 105 or 106 or 107 or 108°C for 3 or 4...

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Abstract

The invention discloses a method for quickly detecting protein and amino acid in rapeseeds. The method comprises the following steps of: A, drying harvested rapeseeds in a drying oven, controlling the humidity for drying at 103 and 108 DEG C, controlling the time between 3 and 5 hours, and then cooling the rapeseeds to the room temperature; B, putting the cooled rapeseeds into a sample tray of a near infrared instrument to scan a near infrared spectrum, and repeating the sample loading for 2 to 4 times each time; C, averaging the spectrums measured by 2 to 4 times of a sample to calculate the average spectrum; and D, substituting the average spectrum into a model to calculating the content of crude protein and the amino acid of the sample. The method is easy to implement, is simple and convenient to operate, and has simple pretreatment of the sample. For a rapeseed manufacturer, the harvested rapeseeds are quickly measured at a certain temperature during a certain time. The method is quick and causes no damages. The acquisition time of the near infrared spectrum is very short, and the model calculation time can be basically ignored. By using the method, a plurality of components can be measured at the same time, and the content of the crude protein and the content of other amino acid in the rapeseeds can be measured at the same time.

Description

technical field [0001] The invention relates to the technical field of detection of proteins and amino acids in rapeseeds, and more specifically relates to a method for detecting proteins and amino acids from rapeseeds, which is applicable to the detection of proteins and amino acids in various rapeseeds. Background technique [0002] Rapeseed is an important source of edible oil for people. With the improvement of people's living standards, cultivating rapeseed with high protein content to improve the comprehensive utilization value of rapeseed has become a direction of rapeseed breeding. How to screen the seeds rich in protein and amino acid from many seeds in the process of seed selection and breeding is an important and complicated project. Traditional chemical and chromatographic analysis methods are complex, time-consuming and labor-intensive. Near-infrared spectroscopy is one of the fastest growing and most compelling spectroscopic analysis techniques. The so-calle...

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Application Information

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IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 傅廷栋谭正林李培武祝利霞孙秀丽
Owner HUAZHONG AGRI UNIV
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