Candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid paper mulberry feed

A technology of UCY-11 and Candida utilis, which is applied in the field of microorganisms to achieve the effects of increasing the content of peptides, improving nutritional quality, and promoting development

Active Publication Date: 2021-01-05
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Candida utilis mainly focuses on its selenium-enriching function, the fermentation and cultivation of Candida utilis to produce single-cell protein feed, a

Method used

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  • Candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid paper mulberry feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of seed liquid: The Candida utilis strain UCY-11 was cultured in the fermented seed liquid medium, and the fermented seed liquid was obtained by culturing it in a fermented seed Erlenmeyer flask. 12r / min;

[0020] (2) Preparation of fermented hybrid paper mulberry medium: the medium is calculated by the following mass percentages: 69% of hybrid paper mulberry dry powder, 10% of corn flour, 5.4% of wheat bran, 5.4% of rice bran, 10% of molasses and inorganic 0.2% salt, the total amount is 100%, mix evenly, and add water to make its mass water content reach 55-60%;

[0021] (3) Preparation of fermented hybrid paper mulberry feed: the fermented seed liquid is inoculated into the fermented hybrid paper mulberry medium according to the inoculation quality of 9%, and put into a triangular flask for fermentation culture, and the loading amount is 40% of the volume of the triangular flask. Fermentation for 60 hours at a temperature of 34°C to obtain wet feed of...

Embodiment 2

[0024] The method that a Candida utilis strain UCY-11 of the present invention is applied in the preparation of fermented hybrid paper mulberry feed is as follows:

[0025] (1) Preparation of seed liquid: The Candida utilis strain UCY-11 was cultured in the fermented seed liquid medium, and the fermented seed liquid was obtained by culturing it in a fermented seed Erlenmeyer flask. 11r / min;

[0026] (2) Preparation of hybrid paper mulberry trunk powder: from a planting base in Henan. In early June, when the whole plant of hybrid paper mulberry grows to a height of 1.0-1.2 m, the first crop is harvested, leaving 15-20 cm of stubble, and the whole plant is dried and crushed To 20~40 meshes, get hybrid paper mulberry trunk powder;

[0027] (3) Preparation of fermented hybrid paper mulberry medium: the medium is made by adding 375 kg of hybrid paper mulberry dry powder, 30 kg of corn flour, 25 kg of wheat bran, 25 kg of rice bran, 50 kg of molasses and 1.5 kg of inorganic salts i...

Embodiment 3

[0031] The method that a Candida utilis strain UCY-11 of the present invention is applied in the preparation of fermented hybrid paper mulberry feed is as follows:

[0032] (1) Preparation of seed liquid: The Candida utilis strain UCY-11 was cultured in the fermented seed liquid medium, and the fermented seed liquid was obtained by culturing it in a fermented seed Erlenmeyer flask. 14r / min;

[0033] (2) Preparation of hybrid paper mulberry trunk powder: from a planting base in Henan. In early June, when the whole plant of hybrid paper mulberry grows to a height of 1.0-1.2 m, the first crop is harvested, leaving 15-20 cm of stubble, and the whole plant is dried and crushed To 20~40 meshes, get hybrid paper mulberry trunk powder;

[0034] (3) Preparation of fermented hybrid paper mulberry medium: the medium is calculated by the following mass percentages: 3500 g of hybrid paper mulberry dry powder, 500 g of corn flour, 250 g of wheat bran, 250 g of rice bran, 500 g of molasses ...

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Abstract

The invention relates to a candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid paper mulberry feed. The candida utilis strain UCY-11 can effectively solve the problems of degrading macromolecular proteins contained in the feed, increasing the content of crude proteins and peptides in the crude proteins, reducing the content of anti-nutritional factors such as cellulose and lignin, improving the nutritional value of the feed and meeting the actual needs of breeding animal husbandry when the fermented hybrid paper mulberry feed is prepared by using the strain.The candida utilis strain UCY-11 is obtained by an ultraviolet mutagenesis technology, and the strain is used for fermenting the hybrid paper mulberry feed, so that the content of the crude proteinsand the peptides in the crude proteins can be effectively increased, the nutritional quality of the hybrid paper mulberry feed is improved, and the utilization efficiency of animals on the hybrid paper mulberry feed is improved; and meanwhile, the intestinal microecological balance of the animals is improved, the development of animal health and breeding industry is promoted, the needs of people on animal proteins in life are met, and the candida utilis strain UCY-11 has remarkable economic and social benefits.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Candida utilis strain UCY-11 and its application in preparing fermented hybrid paper mulberry feed. Background technique [0002] With the rapid development of my country's economy and the continuous improvement of people's living standards, the demand for animal protein is increasing day by day, and the contradiction between supply and demand has become increasingly prominent. Animal protein mainly comes from animal husbandry. Animal husbandry is an important part of modern agriculture in my country and an important source of meat, eggs and milk food. Vigorously developing crude protein plant feed resources has become a new way to alleviate the shortage of protein feed raw materials and ensure food safety. . [0003] Hybrid paper mulberry is a new paper mulberry variety cultivated by the Institute of Botany, Chinese Academy of Sciences using modern biotechnology and traditional h...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N13/00A23K10/12A23K10/18C12R1/72
CPCC12N13/00A23K10/12A23K10/18C12R2001/72C12N1/165
Inventor 张秀江高正龙胡虹王品胜权淑静方玉美刘丽吕文竹向凌云王洋解复红
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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