Candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid paper mulberry feed
A technology of UCY-11 and Candida utilis, which is applied in the field of microorganisms to achieve the effects of increasing the content of peptides, improving nutritional quality, and promoting development
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Embodiment 1
[0019] (1) Preparation of seed liquid: The Candida utilis strain UCY-11 was cultured in the fermented seed liquid medium, and the fermented seed liquid was obtained by culturing it in a fermented seed Erlenmeyer flask. 12r / min;
[0020] (2) Preparation of fermented hybrid paper mulberry medium: the medium is calculated by the following mass percentages: 69% of hybrid paper mulberry dry powder, 10% of corn flour, 5.4% of wheat bran, 5.4% of rice bran, 10% of molasses and inorganic 0.2% salt, the total amount is 100%, mix evenly, and add water to make its mass water content reach 55-60%;
[0021] (3) Preparation of fermented hybrid paper mulberry feed: the fermented seed liquid is inoculated into the fermented hybrid paper mulberry medium according to the inoculation quality of 9%, and put into a triangular flask for fermentation culture, and the loading amount is 40% of the volume of the triangular flask. Fermentation for 60 hours at a temperature of 34°C to obtain wet feed of...
Embodiment 2
[0024] The method that a Candida utilis strain UCY-11 of the present invention is applied in the preparation of fermented hybrid paper mulberry feed is as follows:
[0025] (1) Preparation of seed liquid: The Candida utilis strain UCY-11 was cultured in the fermented seed liquid medium, and the fermented seed liquid was obtained by culturing it in a fermented seed Erlenmeyer flask. 11r / min;
[0026] (2) Preparation of hybrid paper mulberry trunk powder: from a planting base in Henan. In early June, when the whole plant of hybrid paper mulberry grows to a height of 1.0-1.2 m, the first crop is harvested, leaving 15-20 cm of stubble, and the whole plant is dried and crushed To 20~40 meshes, get hybrid paper mulberry trunk powder;
[0027] (3) Preparation of fermented hybrid paper mulberry medium: the medium is made by adding 375 kg of hybrid paper mulberry dry powder, 30 kg of corn flour, 25 kg of wheat bran, 25 kg of rice bran, 50 kg of molasses and 1.5 kg of inorganic salts i...
Embodiment 3
[0031] The method that a Candida utilis strain UCY-11 of the present invention is applied in the preparation of fermented hybrid paper mulberry feed is as follows:
[0032] (1) Preparation of seed liquid: The Candida utilis strain UCY-11 was cultured in the fermented seed liquid medium, and the fermented seed liquid was obtained by culturing it in a fermented seed Erlenmeyer flask. 14r / min;
[0033] (2) Preparation of hybrid paper mulberry trunk powder: from a planting base in Henan. In early June, when the whole plant of hybrid paper mulberry grows to a height of 1.0-1.2 m, the first crop is harvested, leaving 15-20 cm of stubble, and the whole plant is dried and crushed To 20~40 meshes, get hybrid paper mulberry trunk powder;
[0034] (3) Preparation of fermented hybrid paper mulberry medium: the medium is calculated by the following mass percentages: 3500 g of hybrid paper mulberry dry powder, 500 g of corn flour, 250 g of wheat bran, 250 g of rice bran, 500 g of molasses ...
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