Electrochemical immunosensor, preparation method and application thereof

An immunosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of poor specificity of aflatoxin B1 detection, inability to detect and monitor on-site online, and long detection period, and achieve good results. Electrochemical activity and detection ability, meet the problem of rapid on-site detection scene and detection device matching problem, and reduce the effect of experimental instrument limitations

Inactive Publication Date: 2020-01-24
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the current problems of poor detection specificity, low sensitivity, long detection period, and inability to detect and monitor on-site for aflatoxin B1, the present invention provides an electrochemical immunosensor and its preparation method and application

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  • Electrochemical immunosensor, preparation method and application thereof
  • Electrochemical immunosensor, preparation method and application thereof
  • Electrochemical immunosensor, preparation method and application thereof

Examples

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Embodiment 1

[0036] Example 1. Electrochemical immunosensor and its preparation method.

[0037] The preparation steps of the electrochemical immunosensor described in this embodiment are as follows:

[0038] (1) Preparation of carbon fiber, gold electrode modified by gold nanoparticles: select a gold electrode with a diameter of 3mm, drop 5 μl of carbon fiber on its surface (equivalent to about 0.7ul per square millimeter of gold electrode surface), and incubate at 25°C After 90 minutes, rinse the working area of ​​the electrode thoroughly with ultrapure water to remove unbound carbon fiber residues and blow dry with nitrogen, then add 5 μl of nano-gold dropwise, incubate at 25°C for 90 minutes, rinse with ultra-pure water to remove unbound nano-gold and blow dry with nitrogen, Obtain AuNPs / CF / Au electrodes;

[0039] (2) Immobilization of aflatoxin B1 antibody: Add 5 μl of aflatoxin B1 antibody with a mass concentration of 10 μg / mL dropwise on the surface of AuNPs / CF / Au electrode, incuba...

Embodiment 2

[0041] Example 2. Electrochemical immunosensor and its preparation method.

[0042] The preparation steps of the electrochemical immunosensor described in this embodiment are as follows:

[0043] (1) Preparation of carbon fiber and gold electrode modified with gold nanoparticles: based on the electrode area, 0.5 μl of carbon fiber was added dropwise to the surface of gold electrode per square millimeter, incubated at 27°C for 100 min, and the electrode working area was thoroughly rinsed with ultrapure water to remove No bonded carbon fibers remained and dried with nitrogen, then 0.5 μl nano-gold was added dropwise on the surface of gold electrode per square millimeter, incubated at 27°C for 100 min, washed with ultrapure water to remove unbound nano-gold and dried with nitrogen to obtain AuNPs / CF / Au electrode;

[0044] (2) Immobilization of aflatoxin B1 antibody: based on the electrode area, add 0.5ul of aflatoxin B1 antibody with a mass concentration of 20μg / mL per square m...

Embodiment 3

[0046] Example 3. Electrochemical immunosensor and its preparation method.

[0047] The preparation steps of the electrochemical immunosensor described in this embodiment are as follows:

[0048] (1) Preparation of carbon fiber and gold electrode modified by gold nanoparticles: based on the electrode area, 1 μl of carbon fiber was added dropwise to the surface of gold electrode per square millimeter, incubated at 23°C for 120 min, and the working area of ​​the electrode was thoroughly rinsed with ultrapure water to remove any impurities. The bound carbon fibers remained and dried with nitrogen, and then 1 μl of nano-gold was dropped on the surface of gold electrode per square millimeter, incubated at 23°C for 120 min, washed with ultrapure water to remove unbound nano-gold and dried with nitrogen to obtain AuNPs / CF / Au electrode ;

[0049] (2) Immobilization of aflatoxin B1 antibody: based on the electrode area, add 1 ul of aflatoxin B1 antibody with a mass concentration of 30...

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Abstract

An electrochemical immunosensor, a preparation method and application thereof belong to the technical field of electrochemical immunosensors. In order to solve the problems of poor specificity, low sensitivity, long detection cycle and impossibility of in-situ online detection and monitoring of aflatoxin B1, the invention provides an electrochemical immunosensor, a preparation method and application thereof. The preparation method of the electrochemical immunosensor comprises the following steps of (1) preparing carbon fibers and gold nanoparticles modified gold electrodes; (2) immobilizing aflatoxin antibodies; and (3) blocking non-specific binding sites by BSA to obtain a standard curve, wherein y=0.1296+0.03774x, the linear correlation coefficient R2=0.98858, and the linear detection range of the curve is 0.01-1000ng/mL. The sensor provided by the invention can measure the content of aflatoxin B1 at a small range of voltage of about 0.11V or at an exact voltage.

Description

technical field [0001] The invention belongs to the technical field of electrochemical immunosensors, and in particular relates to an electrochemical immunosensor and its preparation method and application. Background technique [0002] Aflatoxins are toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and parasitic bacteria, and are the most toxic and most toxic of mycotoxins. Since aflatoxins are frequently found in food and agricultural products, they cause serious health and economic problems in many countries. Among the aflatoxins, aflatoxin B1 (AFB1) is the most toxic and is listed as a class I carcinogen by the International Agency for Research on Cancer. Therefore, it poses a great threat to the health of people, livestock, and poultry, which has attracted great attention from all countries in the world, and has formulated the maximum limit as a mandatory standard for food and feed. [0003] At present, there are many methods for the detecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N33/53
CPCG01N27/308G01N27/3277G01N27/3278G01N33/53G01N2333/38
Inventor 赵文苹孙霞刘中合郭业民赵亚
Owner SHANDONG UNIV OF TECH
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