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Method for regenerating plant by use of callus of camellia japonica

A technology for callus and plant regeneration, applied in the biological field, can solve problems such as unseen patent applications

Active Publication Date: 2013-05-08
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, there are already examples of obtaining Naidong camellia through the method of "bud sprouting buds" stem segment culture, but there are few reports on the case of Naidong camellia induced by callus to the differentiation of adventitious buds, and no patent application has been seen.

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  • Method for regenerating plant by use of callus of camellia japonica

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with specific examples.

[0024] 1) Collect the fruits of Naidong camellia in early September, peel off the peel, scrub the seeds with detergent, then scrub with tap water until there is no foam, put them in a culture bottle, add 75% alcohol for the first disinfection and cleaning for 8-12 seconds , then wash with pure water 2-3 times, drain the water, cover the bottle cap, and put it in a sterile ultra-clean bench for later use;

[0025] 2) Disinfect the seed surface again with 75% alcohol for 25-35 seconds, followed by 0.1% HgCl 2 Soak for 6-10 minutes, then rinse with sterile water 5-6 times, after cleaning, spread the seeds on a plate with filter paper to dry the water, and set aside;

[0026] 3) Peel off the inner and outer testa of the seeds, and inoculate them on the callus induction medium, the culture temperature is 25±2°C, the light intensity is 2500-3000 Lx, and the light time is 10-12...

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Abstract

The invention discloses a method for regenerating a plant by use of the callus of camellia japonica, belonging to the field of biotechnology. The method is characterized by comprising the following steps of: collecting the fruits of camellia japonica in early September, and disinfecting and cleaning; removing the inner and outer seed coats of seeds, and inoculating to a callus induction medium; transferring the material to a callus transition medium in the 25th -28th day; after the callus turns from light yellow into yellow with a little red, transferring the material into a callus differentiation adventitious bud medium; after the material is formed and bud sticks of 3-4cm grow, soaking the base part in IBA, and transferring to a paper bridge of an MV liquid medium to induce rooting; starting in the 20-22 days to grow transparent radicles with a little red; and then culturing for a certain period of time so that the radicels extend and are gradually lignified. The method for regenerating a plant by use of callus of camellia japonica, disclosed by the invention, provides a process with a relatively short period and a relatively high reproduction coefficient to large-scale asexual reproduction of camellia japonica, and lays a foundation for the molecular breeding of camellia japonica.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for regenerating plants from callus of Naidong camellia. Background technique [0002] Naidong camellia is a remnant plant species of the Tertiary period. Due to the movement of the earth's crust, there was little gene exchange between Qingdao Naidong and the outside world, thus retaining the ancient and unique genetic characteristics. From an ornamental point of view, Naidong camellia has bright colors and beautiful flower shapes, and is the city flower of Qingdao; at the same time, its leaves and stems can also be used as medicine for the prevention and treatment of various diseases, which has great medical application prospects; not only that, Naidong Dongshan tea also has strong CO 2 absorption capacity, and for H 2 Harmful gases such as S have strong resistance, so it also has environmental protection effects such as purifying the air, which can be described as a tre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李纪元范正琪李辛雷殷恒福吴斌
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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