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Sterile grafting method for solving problem that barbadosnut converted adventitious budling can hardly take root

A technology for Jatropha curcas and sprouts is applied in the fields of plant biotechnology and crop genetics and breeding, and can solve the problems of immature aseptic grafting technology and difficult rooting of adventitious sprouts, achieve a good degree of healing, solve difficult rooting, and improve quality Effect

Inactive Publication Date: 2014-04-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is in order to overcome the defect that Jatropha curcas transforms adventitious shoots into roots difficultly and the existing aseptic grafting technology is immature in the prior art, and provides a solution aseptic grafting method

Method used

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  • Sterile grafting method for solving problem that barbadosnut converted adventitious budling can hardly take root
  • Sterile grafting method for solving problem that barbadosnut converted adventitious budling can hardly take root
  • Sterile grafting method for solving problem that barbadosnut converted adventitious budling can hardly take root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: The effect of antibiotics on the growth of Jatropha curcas grafted seedlings

[0031] In this study, antibiotics were added to the culture medium to explore the effect of antibiotics on the growth and development of Jatropha curcas grafted seedlings. In this experiment, grafted seedlings were inoculated on MS medium without antibiotics, 200 mg / l carbenicillin or 200 mg / l cephalosporin, and the growth of grafted seedlings was observed and recorded after 0 days and 30 days of culture. The results are as follows Table 1 shows.

[0032] Table 1 The effect of antibiotics on the growth of grafted seedlings

[0033]

[0034] Note: The data uses SPSS Statistics 17.0 statistical analysis software for analysis of variance and Duncan's multiple comparison (P ≦ 0.05). Different letters after the data indicate significant differences between treatments. Graft survival rate (%) = (number of grafted seedlings survived / total number of grafted seedlings) × 100%; increase in plant ...

Embodiment 2

[0036] Example 2 The effect of rootstocks obtained from different germination days on the growth of grafted seedlings

[0037] After conventional sterilization, the husked seeds of Jatropha curcas were inoculated on hormone-free MS medium and cultured for 5 days, 15 days, and 30 days later. The seedlings were cut separately from the seedling cotyledon about 5 mm below the cotyledon. The hypocotyl of the intact radicle was used as the rootstock material for grafting. The scion was grafted onto the rootstock, and the growth of the grafted seedlings was observed and recorded after 0 days and 30 days of culture. The results are shown in Table 2.

[0038] Table 2 The effect of days of germination on the growth and development of grafted seedlings

[0039]

[0040] Note: The data uses SPSS Statistics 17.0 statistical analysis software for analysis of variance and Duncan's multiple comparison (P ≦ 0.05). Different letters after the data indicate significant differences between treatments. ...

Embodiment 3

[0042] Example 3 The effect of sodium nitroprusside (SNP) on the growth and development of Jatropha curcas grafted seedlings

[0043] There are reports in the literature that SNP can promote rooting and leaf growth of some plants (Han et al, 2009 and Xu et al, 2009). This experiment explored the effect of SNP on the growth of Jatropha curcas grafted seedlings. After conventional sterilization, the husked seeds of Jatropha curcas were inoculated on hormone-free MS medium and cultured for 5 days. The seedlings were cut and cut from a position about 5 mm below the cotyledon of the seedlings, and the hypocotyls with radicles were taken As the rootstock material for grafting (about 1.5 cm in length), two types of scion (bud and sprout) are grafted onto the rootstock, and the grafted seedlings are inoculated into MS culture with 0, 1, 3 mg / l SNP Basically, the growth of grafted seedlings was observed and recorded after 0 days and 30 days of culture. The results are shown in Table 3. ...

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Abstract

The invention belongs to the field of crop genetic engineering and relates to a sterile grafting method for solving the problem that barbadosnut converted adventitious budling can hardly take root. The sterile grafting method includes that the barbadosnut converted adventitious budling obtained by screening is taken as scions which are grafted to a hypocotyledonary axis serving as a stock and provided with part of radicle primordium, and the scions and the stock after grafted are good in degree of healing. By the application of the sterile grafting method, the problem that the barbadosnut converted adventitious budling can hardly take root can be well solved, and significant roles in obtaining more barbadosnut converted seedlings and improving quality of the same are played.

Description

technical field [0001] The invention relates to the fields of plant biotechnology and crop genetic breeding, in particular to an aseptic grafting method for solving the problem of rooting of Jatropha curcas transformed adventitious shoots. Background technique [0002] Plant genetic transformation is an important and advanced breeding technology. Due to the high oil content (40-60%) in its seeds, Jatropha curcas can be developed into "biodiesel", thus becoming a very promising energy plant, but its distribution area is narrow and the seed setting rate is low , the quality of seed oil also needs to be further improved. In order to expand the planting area of ​​Jatropha curcas, new varieties of Jatropha curcas with high yield and high quality have been bred, and genetic transformation technology can be used to achieve these goals. At present, the technology of plant genetic transformation has been basically mature, and the most common transformation is to introduce the exogen...

Claims

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Application Information

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IPC IPC(8): A01G1/06A01H4/00
Inventor 杨跃生刘颖刘振兰庄楚雄李静惠文凯陈晓阳
Owner SOUTH CHINA AGRI UNIV
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