A kind of aseptic grafting method that solves the problem of Jatropha curcas transformed adventitious shoots being difficult to take root

A technology for Jatropha curcas and sprouts is applied in the fields of plant biotechnology and crop genetics and breeding, and can solve the problems of immature aseptic grafting technology and difficult rooting of adventitious sprouts, achieve a good degree of healing, solve difficult rooting, and improve quality Effect

Inactive Publication Date: 2015-10-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is in order to overcome the defect that Jatropha curcas transforms adventitious shoots into roots difficultly and the existing aseptic grafting technology is immature in the prior art, and provides a solution aseptic grafting method

Method used

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  • A kind of aseptic grafting method that solves the problem of Jatropha curcas transformed adventitious shoots being difficult to take root
  • A kind of aseptic grafting method that solves the problem of Jatropha curcas transformed adventitious shoots being difficult to take root
  • A kind of aseptic grafting method that solves the problem of Jatropha curcas transformed adventitious shoots being difficult to take root

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: the impact of antibiotics on the growth effect of Jatropha curcas grafted seedlings

[0031] In this study, the effects of antibiotics on the growth and development of Jatropha curcas grafted seedlings were explored by adding antibiotics to the medium. In this experiment, the grafted seedlings were inoculated on the MS medium without adding antibiotics, adding 200 mg / l carbenicillin or adding 200 mg / l cephalosporin, and observed and recorded the growth of grafted seedlings after 0 days and 30 days of culture. The results are as follows: Table 1 shows.

[0032] Table 1 Effect of antibiotics on the growth of grafted seedlings

[0033]

[0034]Note: SPSS Statistics 17.0 statistical analysis software was used for data analysis of variance and Duncan's multiple comparisons (P ≦ 0.05). Different letters after the data indicate significant differences between treatments. Grafting survival rate (%) = (number of surviving grafted seedlings / total number of graf...

Embodiment 2

[0036] The rootstock that embodiment 2 different germination days obtains is to the influence of grafted seedling growth effect

[0037] After routine sterilization, the shelled seeds of Jatropha curcas were inoculated on the hormone-free MS medium and cultured for 5 days, 15 days, and 30 days. The hypocotyl of the complete radicle was used as the rootstock material for grafting, and the scion was grafted onto the rootstock, and the growth conditions of the grafted seedlings were observed and recorded after culturing for 0 days and 30 days, and the results are shown in Table 2.

[0038] The influence of table 2 germination days on the growth and development of grafted seedlings

[0039]

[0040] Note: SPSS Statistics 17.0 statistical analysis software was used for data analysis of variance and Duncan's multiple comparisons (P ≦ 0.05). Different letters after the data indicate significant differences between treatments. Grafting survival rate (%) = (number of surviving gr...

Embodiment 3

[0042] Example 3 Effect of sodium nitroprusside (SNP) on the growth and development of Jatropha curcas grafted seedlings

[0043] It has been reported in the literature that SNPs can promote rooting and leaf growth in some plants (Han et al, 2009 and Xu et al, 2009). This experiment explored the effect of SNP on the growth effect of Jatropha curcas grafted seedlings. Jatropha curcas shelled seeds are routinely sterilized, inoculated on hormone-free MS medium and cultured for 5 days, then the seedlings are cut from a position about 5 mm below the cotyledons of the seedlings, and the hypocotyls with radicles are taken As the rootstock material for grafting (about 1.5 cm in length), two types of scions (buds and shoots) were grafted onto the rootstock, and the grafted seedlings were inoculated into MS culture supplemented with 0, 1, and 3 mg / l SNP On the base, observe and record the growth of grafted seedlings after culturing 0 day and 30 days respectively, and the results are...

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Abstract

The invention belongs to the field of crop genetic engineering and relates to a sterile grafting method for solving the problem that barbadosnut converted adventitious budling can hardly take root. The sterile grafting method includes that the barbadosnut converted adventitious budling obtained by screening is taken as scions which are grafted to a hypocotyledonary axis serving as a stock and provided with part of radicle primordium, and the scions and the stock after grafted are good in degree of healing. By the application of the sterile grafting method, the problem that the barbadosnut converted adventitious budling can hardly take root can be well solved, and significant roles in obtaining more barbadosnut converted seedlings and improving quality of the same are played.

Description

technical field [0001] The invention relates to the fields of plant biotechnology and crop genetic breeding, in particular to an aseptic grafting method for solving the problem of rooting of Jatropha curcas transformed adventitious shoots. Background technique [0002] Plant genetic transformation is an important and advanced breeding technology. Due to the high oil content (40-60%) in its seeds, Jatropha curcas can be developed into "biodiesel", thus becoming a very promising energy plant, but its distribution area is narrow and the seed setting rate is low , the quality of seed oil also needs to be further improved. In order to expand the planting area of ​​Jatropha curcas, new varieties of Jatropha curcas with high yield and high quality have been bred, and genetic transformation technology can be used to achieve these goals. At present, the technology of plant genetic transformation has been basically mature, and the most common transformation is to introduce the exogen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/06A01H4/00
Inventor 杨跃生刘颖刘振兰庄楚雄李静惠文凯陈晓阳
Owner SOUTH CHINA AGRI UNIV
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