48 results about "Jatropha curcas x Jatropha integerrima" patented technology

The invention discloses a method for preparing active carbon with waste barbadosut shells, and is characterized in that: the waste barbadosut shells are firstly sieved and crumbled, then carbonization and activation are carried out by a physical method or a chemical method so as to obtain the active carbon. The invention not only has abundant raw material sources, so nonrenewable resources such as coal, tar and valuable wood materials that are used commonly at present can be saved, but also has low product cost, high added value, good performance and obvious market competition advantages, besides, the environment pollution problems caused by stack or burning of the waste barbadosut shells are solved, therefore, a new way of taking use of the resources fully is found, meanwhile, an important link is filled for the production mode of circular economy characterized in low investment, high efficiency and zero pollution of the waste barbadosut shells.

The invention discloses a Jatropha curcas mycorrhizal fungi compound fertilizer, which comprises one or more arbuscular mycorrhizal fungi containing glomus and gigaspora that are served as the mycorrhizal fungi. The compound fertilizer consists of 20-30 percent of the mycorrhizal fungi, 1-12 percent of phosphate fertilizer, 2-18 percent of potassium fertilizer, 8-15 percent of nitrogenous fertilizer, 2-3 percent of zinc sulfate and 20-50 percent of humus soil. The compound fertilizer of the invention can improve the absorption ratio of Jatropha curcas root absorbing soil nutrition, meet the growth, development and fruit bearing of the Jatropha curcas, improve soil ecological environment and increase seed yield.

The invention belongs to the technical fields of molecular biology and genetic engineering, and provides an encoding sequence for encoding cDNA of Jatropha curcas farnesyl pyrophosphate synthase. The farnesyl pyrophosphate synthase catalyzes isopentenyl pyrophosphate and dimethylallyl pyrophosphate to carry out continuous condensation reaction to form farnesyl pyrophosphate, is key enzyme for metabolic pathway of Jatropha curcas isoprene, and contributes to reducing the content of phorbol esters and precursor thereof in the Jatropha curcas. Due to the use of the Jatropha curcas protein, substances interacting with the Jatropha curcas protein, or receptors, inhibitors or antagonists, and the like can be screened by various conventional screening methods. The encoding sequence for encoding the cDNA of the Jatropha curcas farnesyl pyrophosphate synthase has great application prospects.

The invention discloses a jatropha curcas dedicated composite fertilizer, a preparation method and applications thereof. The dedicated composite fertilizer is prepared from the following components in parts by weight: 30 to 35 parts of banana stalk, 35 to 40 parts of manure of livestock and poultry, 5 to 12 parts of liquid monosodium glutamate dreg, 10 to 13 parts of peanut bran powder, 1 to 3 parts of jatropha bran powder, 0.02 to 2 parts of plant insecticide, 1 to 2 parts of water-retaining agent, 0.002 to 0.010 part of microbial inoculant, 1 to 2 parts of urea with a nitrogen content not less than 46.4%, 3 to 4 parts of calcium superphosphate with a P2O5 content not less than 12%, and 2 to 3 parts of potassium chloride with a K2O content not less than 60%. The provided fertilizer is suitable for being used in the seedling period and topdressing period and can be used as a base fertilizer. The jatropha curcas dedicated composite fertilizer has comprehensive and abundant nutrients, can provide enough nutrients for jatropha curcas, and is capable of preventing diseases and insects. Jatropha curcas is usually planted in water-deficient areas such as hillside, high mountains, and the like, and the provided fertilizer can relieve water deficiency. Moreover, the raw material source is wide, the cost is low, and the preparation technology is simple. So the fertilizer can promote the sustainable development of rock desertification region and generates good economic benefit, social benefit, and ecological benefit.

The invention discloses a remediation method for an acid heavy metal contaminated soil. The method mainly uses a botanical Jatropha curcas which has outstanding tolerance to extreme soil environments featured by high-concentration heavy metal residuals, being lacking of so many nutrient elements, having poor soil texture and the like; and in the method, seedlings are transferred in the contaminated place for field planting after starting seeds indoors so as to steadily remedy the contaminated soil. After being remedied by the Jatropha curcas, pH of the soil is improved, biological available heavy metal is greatly reduced, and harm for the environment is greatly relieved, so that the method has obvious ecological benefits. And simultaneously, production value per mu of bioenergy in each year is at least RMB 2850 yuan, so that the method has obvious economic benefits.

The invention specifically discloses a method for direct regeneration of an adventitious bud from a Jatropha curcas petiole explant, which belongs to the field of plant biotechnology. The method improves a traditional method for regeneration of the adventitious bud from the Jatropha curcas petiole explant; i.e., instead of addition of hormone into a culture medium which induces regeneration of the adventitious bud, a high-concentration thidiazuron solution is used for short-period soaking treatment of the Jatropha curcas petiole explant so as to give short-period but high-intensity hormone stimulation to explant cells, thereby allowing a part of the cells to undergo more highly efficiently direct redifferentiation so as to form more adventitious buds. With the method provided by the invention, regeneration efficiency and regeneration quality of the adventitious bud from the Jatropha curcas petiole explant are substantially improved. Moreover, the explant used in the method does not need to undergo routine stages of callus formation and adventitious bud propagation, so a regeneration culture period is substantially shortened, and the efficiency of operations related to biotechnological breeding of Jatropha curcas is greatly improved accordingly.

The invention discloses a method for extraction and separation purification of phorbol ester components of jatropha curcas seeds and a use of the phorbol ester components. Phorbol ester components of jatropha curcas are subjected to ethyl acetate extraction and column chromatography so that total phorbol ester components are obtained. The total phorbol ester components are treated by a semipreparative high-performance liquid chromatography so that five phorbol ester monomer components are obtained. The method provided by the invention provides a foundation for further exploitation and utilization of phorbol ester components of jatropha curcas.

The invention discloses a cleft grating method for jatropha curcas seedlings. The method includes the steps of soil preparation, sowing, nursery stock management, cleft grating time, a cleft grating method, cleft grating post-management and afforestation. The jatropha curcas seedlings cultivated as stock are fast and even in growing, good in a growing way and free of disease and insect pest infection. Germination rate and unbinding survival rate of once cleft grating are as high as 96.5%. When cleft grating is conducted 20 days before germinating of jatropha curcas in spring, blooming rate and yield can reach 80% at the end of June and can reach 100% next year. After the cleft grating technology is adopted for propagation, an initial period of flowering is 2 years earlier than common seedling planting. The method is a good propagation method for good varieties of the jatropha curcas at present.

The invention discloses a detoxification method of Jatropha curcas cake by fermentation, which comprises the following steps: (1) fetching Enterobacter gergoviae and Morganella morganii, respectively recovering and preparing Enterobacter gergoviae seed liquid and Morganella morganii seed liquid, and mixing the seed liquids according to a ratio 1:2-2:1 (v / v), and obtaining a seed liquid; (2) fetching a Jatropha curcas cake, adding water and carbon source, mixing well, inoculating the seed liquid prepared in the step (1), wherein, the ratio between the material and the water is 1:0.8-1.2 (w / v), and the addition of the carbon source is 10-20% (w / w) of the Jatropha curcas cake. The inoculation amount of the seed liquid is 10-30% (v / w) of the Jatropha curcas cake; (3) the inoculated cake is fermented for 2-8 days at 30-37 DEG C. The invention can effectively reduce toxicity of the Jatropha curcas cake, and simultaneously improve the nutrition value with good market application prospect.

The invention belongs to the field of plant genetic engineering technology and specifically discloses an Agrobacterium tumefaciens-mediated genetic transformation method applied to cotyledon explant transformation receptor system of Jatropha curcas. According to the invention, high-concentration TDZ (thidiazuron) is used for short-term soaking of cotyledon explant, then low-concentration Agrobacterium tumefaciens bacterial liquid is used for short-term invasion of the cotyledon explant, so the genetic transformation rate of Agrobacterium tumefaciens can be substantially improved; the invaded explant is subjected to regeneration culture, resistant buds directly undergo PCR identification, and then micro-grafting can be carried out so as to obtain a transgenic plant. The method provided by the invention greatly improves the genetic transformation rate and substantially shortens a period for obtainment of a completely transformed plant.

The invention relates to a method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester), which comprises the following steps: a preprocessing step: collecting jatropha seeds, and preprocessing the jatropha seeds; a mutagenizing step: mutagenizing the jatropha seeds for an appropriate time by using an EMS solution (with appropriate concentration), and in the process of chemical mutagenizing, simultaneously mixing the seeds with the solution so as to ensure that the EMS solution is uniform in concentration and the jatropha seeds can be fully contacted with the EMS solution; a seedling raising and transplanting step: carrying out seedling raising and timely transplanting on the jatropha M_1 seeds subjected to mutagenizing so as to cultivate jatropha M_1 plants; and a self-pollination step: after the cultivated jatropha M_1 plants reach the flowering stage, carrying out self-pollination on the jatropha M_1 plants, after the fruits of the jatropha M_1 plants get ripe, obtaining the jatropha M_2 seeds. The method in the invention has the advantages that an ethylmethane sulfonate is used as a mutagen for mutagenizing the jatropha seeds, the preprocessing step and the mixing and self-pollination are adopted in the process of mutagenizing, the jatropha M_2 seeds processed by EMS can rapidly be obtained.

The invention relates to a jatropha curcas oil acrylate polymer fatliquor prepared based on a miniemulsion polymerization method and a method for preparing the jatropha curcas oil acrylate polymer fatliquor. The method comprises the following steps: epoxidizing jatropha curcas oil by using hydrogen peroxide, glacial acetic acid and concentrated sulfuric acid to obtain epoxy jatropha curcas oil; taking sodium p-toluenesulfonate as a catalyst, hydroquinone as a polymerization inhibitor and acrylic acid as a nucleophilic reagent to carry out ring-opening reaction on the epoxy jatropha curcas oilto obtain jatropha curcas oil acrylate and introducing an active vinyl part into a vegetable oil molecular chain; dissolving sodium dodecyl sulfate and n-butyl alcohol into deionized water, carrying out ultrasonic uniformity to form an aqueous phase, mixing the aqueous phase with an oil phase, carrying out shearing emulsification and ultrasonic operation to obtain faint yellow stable miniemulsion;adding maleic anhydride, a catalyst, acetanilide and acrylic acid into the miniemulsion for carrying out polymerization reaction to obtain the jatropha curcas oil acrylate polymer fatliquor. The fatliquor has better cold water emulsibility and hydrophilicity, and is a green environmental and biodegradable fatliquoring material.

The invention discloses a fluorescent quantum dot marked chitosan DNA nano composite vector and preparation and application thereof, and particularly aims at transduction of jatropha curcas genes. The method comprises the following steps of: preparing CdSe fluorescent quantum dots with good water solubility and good biological affinity, and preparing the chitosan DNA nano vector by using a complex coacervation method. The quantum dots are combined to the chitosan DNA nano vector under the action of static electricity to form a fluorescent nano complex using the vector as a center and encircled by the quantum dots. The preparation method is simple and feasible; the obtained complex is uniform and has enhanced fluorescent property and good stability; and when the complex is used for the transduction of jatropha curcas cells, fluorescent expression signals of intracellular report genes (GFP protein) can be successfully detected, and the complex can be widely used for the transduction of other plant genes.

A process for preparing a food or feed composition from J. curcas is disclosed. The method involves adding an acidified aqueous solution to J. curcas components, to a final pH of between 1 and 5, incubating the acidified mixture for a period for a period of at least 1 hour, and centrifuging the incubated mixture to separate the mixture into three physically distinct fractions: (i) a light, upper fraction containing oil, (ii) an aqueous fraction containing soluble acid-extracted components and breakdown products, and (iii) a substantially detoxified solid cake which forms or is used in forming the food or feed composition. The acidified aqueous solution added may be acidified olive vegetation water having a ratio of hydroxytyrosol to oleuropein of between 5:1 to 100:1. Also disclosed are a food or feed composition, and oil and aqueous fractions formed by the method.

The invention discloses a method for preparing biodiesel by catalyzing jatropha curcas oil with zirconium-based MOFs material loaded ionic liquid. The method comprises the steps of synthesis of zirconium-based MOFs, preparation of ionic liquid, preparation of the zirconium-based MOFs supported ionic liquid and preparation of the biodiesel; the prepared composite catalyst can be conveniently recycled and stored, can also be directly used for preparing biodiesel from the catalyst, is green and pollution-free, and meets the requirements of green chemistry.

The invention discloses a jatropha curcas alkane type diterpenoid compound, a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: firstly extracting euphorbia helioscopia by using an ethanol aqueous solution to obtain a crude extract, and then sequentially carrying out extraction, ODS chromatographic column and HPLC chromatographic multi-stage separation to obtain the jatropha curcas alkane type diterpenoid compound. According to the invention, the preparation method of the jatropha curcas alkane type diterpenoid compound is simple, easy to operate, convenient for industrial large-scale production, and suitable for industrial large-scale production of the jatropha curcas alkane type diterpenoid compound; and the jatropha curcas alkane type diterpenoid compound prepared by the invention has obvious cytotoxicity to a drug-resistant cell line A549 / Taxol, and is expected to be used as a potential drug for treating drug-resistant lung cancer.

The invention belongs to the technical field of plant biology, and particularly discloses a method for promoting a jatropha curcas explant to regenerate adventitious buds. The traditional jatropha curcas explant adventitious bud regeneration method is changed by the method, that is hormone is not added to a culture medium for inducing the adventitious buds to regenerate any longer, while a high-concentration thidiazuron solution is used for performing short-term soaking treatment on the jatropha curcas explants of various sources, and short-term and high-strength hormone stimulation is applied on explant cells, so that partial cells re-differentiate directly with higher efficiency to form more adventitious buds. The regeneration efficiency and the quality of the adventitious buds of the jatropha curcas explant can be improved significantly by using the method. In addition, the explant in the method is not required to be subjected to conventional caluus formation or an adventitious bud multiplication stage, so that a regeneration culture cycle can be shortened significantly, and the working efficiency of jatropha curcas related biotechnology breeding is improved correspondingly and greatly.

The invention discloses a treatment method for slowing gully erosion, and belongs to the technical field of water and soil conservation. The method comprises four parts, namely gully selection, gully head treatment, gully bottom treatment and gully head wall treatment. In the gully, the slope of the left gully wall or the gully wall is less than or equal to 45 degrees and the height of the gully head wall is less than or equal to 5 meters; jatropha curcas and dodonaea viscose are planted above the gully head to form an inverted U-shaped buffer plant hedge belt above the gully head; timber piles are embedded into the plant hedge belt crookedly; the width of an inverted U-shaped opening is equal to that of the gully head or the width is 1 to 1.5 meters; the top of the inverted U-shaped curve is 3 to 5 meters away from the gully head; leucaena glauca are planted on the gully bottom to form a U-shaped buffer plant hedge belt on the gully bottom; the bottom of the U-shaped curve is 3 to 5 meters away from the gully head; lianas are planted and the timer piles are embedded into the soil between the buffer plant hedge belts above the gully head and the gully bottom; and the two ends of iron wires are respectively fixed to the timber piles at the gully bottom and above the gully head and are attached to the ground surface. In the method, key points of slowing the gully erosion are grasped; and the treatment configuration mode has high comprehensiveness, scientificity and reasonability.

The invention relates to the technical fields of molecular biology and genetic engineering and relates to a JcPSY protein for expressing the Jatropha curcas phytoene synthetase and a nucleotide sequence thereof. Due to the use of the Jatropha curcas PSY protein, substances interacting with the Jatropha curcas PSY protein, or receptors, inhibitors or antagonists, and the like can be screened by various conventional screening methods. The Jatropha curcas PSY protein genes can be used for improving the beta-carotene content in the plants and improving the economic values of the plants through the genetic engineering technology, and have obvious market application prospects.

The invention provides a preparation method and an application of a jatropha curcas leaf extract feed additive. The method comprises the following steps of: drying collected fresh jatropha curcas leaves in the shade; preparing the jatropha curcas leaves dried in the shade into jatropha curcas leaf powder; evenly mixing the jatropha curcas leaf powder and ethyl alcohol in a conical flask (a solid-liquid ratio is 1g:40mL) to obtain the mixed liquor of the jatropha curcas leaf and the ethyl alcohol; putting the mixed liquor of the jatropha curcas leaf and the ethyl alcohol in a constant-temperature water bath shaking table at a temperature of 50DEG C, carrying out suction filtration after the mixed liquor is placed for 80min, and then, carrying out centrifugation by a high-speed refrigeratedcentrifuge at a temperature below 4DEG C for 10min, and collecting supernate; and transferring the supernate to a rotary evaporator, and carrying out concentration processing to obtain concentrated concrete. The jatropha curcas leaf extract is used as the feed additive which has an effect similar to an antibiotic feed additive, the antibiotic feed additive can be replaced, livestock is acceleratedto grow, and the intestinal health of the livestock is regulated and controlled.

The invention discloses a Jatropha Curcas transcription factor, its encoding gene and use in cultivating plants with increased resistance. The transcription factor is the protein having one of the following amino acid residue sequences: (1) SEQ ID No.1 in the sequence table, (2) protein with plants resistance regulation of transcription activation function obtained through subjecting SEQ ID No.1 amino acid sequences in the sequence table to 1-10 amino acid sequences substitution, deletion or addition.

The invention provides jatropha curcas L. ribosome-inactivating protein and a preparation method thereof. The jatropha curcas L. ribosome-inactivating protein is separated and purified from cotyledon and / or endosperm of jatropha curcas L. seedlings which are generated by jatropha curcas L. seeds germinating for 4 to 49 days, relative molecular weight is 31.398kDa, an isoelectric point is 7.12, and a sequence of amino acid residue of an N-terminal of the ribosome-inactivating protein is shown as SEQ ID No: 1 in a sequence table. The invention further provides application of the jatropha curcas L. ribosome-inactivating protein in preparing medicines for treating tumor. The jatropha curcas L. ribosome-inactivating protein has an obvious anti-proliferative effect on human osteosarcoma U20S, human non-small cell lung cancer A549, human non-small cell lung cancer H1975 and human colon cancer HCT116; an in-vitro proliferation inhibition effect of the jatropha curcas L. ribosome-inactivating protein on the human osteosarcoma U20S is superior to that of Curcin and taxol; furthermore, toxicity to human normal kidney cells is lower.

The present invention relates to the field of somatic embryo production, particularly to methods for the regeneration of Jatropha through somatic embryogenesis. More specifically, the present invention relates to a method and media compositions for regeneration of plants of Jatropha curcas. The method is well suited for Jatropha curcas transformation and for producing clonal planting stock useful for large scale Jatropha curcas plantation.

Jatropha curcas plants can be produced that are characterized by an inflorescence with female-only (FO) flowers. Such plants are especially useful for interplanting in order to obtain commercial scale production of new J curcas hybrids derived from the seeds of the FO-type plant.

The invention belongs to the field of pesticides, and specifically relates to a jatropha curcas essence micro-emulsion and a preparation technology thereof. According to the invention, a plurality of emulsifiers, auxiliary solvents, synergists and stabilizing agents are screened, and the jatropha curcas essence micro-emulsion with good stability and good dispersity is prepared. The technology is characterized in that: the jatropha curcas essence micro-emulsion is prepared from components of: 1-40% of jatropha curcas essence (wherein the active component is jatropherol I), 1-30% of an emulsifier, 4-13% of a stabilizing agent, 0.1-5% of a synergist, 10-20% of an auxiliary solvent, 0.01-1% of a pH regulatior, 0.1-5% of an anti-freezing agent, and balance of water. The pesticide provided by the invention is a micro-emulsion with water as a solvent. Therefore, insecurity during production, application, storage and transportation caused by over-commitment of organic solvents is avoided. The micro-emulsion provided by the invention is characterized in high safety and environment friendliness.

The invention discloses Jatropha curcas L. diacylglycerol transferase (Jcdagat) and a coding gene and application thereof and belongs to the technical field of genetic engineering. The amino acid residue sequence of the Jcdagat is shown in SEQ ID No:2, or the amino acid residue sequence has 95% of homology with the amino acid residue sequence shown in SEQ ID No:2 and has the protein which is derived from SEQ ID No:2 and has the same activity or the protein which is derived from SEQ ID No:2 by replacing, deleting or adding one or more of amino acids to the amino acid in SEQ ID No:2 and has the same function. The invention also discloses a gene coding diacylglycerol transferase. Experiments show that the transgene disclosed by the invention can be utilized to significantly increase the oil content of plants or algae, thus the high-quality gene can be provided for the improvement of the oil content of plants or algae and have wide application prospect and important application value.

The invention discloses a compound pickling corrosion inhibitor of jatropha curcas cake extract and a preparation method thereof, which belong to the technical field of corrosion inhibitors. The present invention optimizes the process of extracting acid corrosion inhibitor from jatropha curcas cake, removes toxic protein and phorbol ester, overcomes the problem that the acid corrosion inhibitor extracted from jatropha curcas cake is toxic, and uses pomelo peel extract and leprosy The tree cake extract has a synergistic effect, high corrosion inhibition efficiency, strong adaptability, and obviously inhibits the corrosion effect of acid on steel and aluminum materials, and more importantly, it has a good corrosion inhibition effect on zinc, zinc and aluminum alloys. The corrosion inhibitor of the invention is safe, harmless, biodegradable, and easy to use, and can be applied to cleaning petrochemical equipment, boilers, and even tap water pipelines, and has good development prospects.

The invention discloses an environment-friendly enzyme disinfectant and a preparation method and application thereof. The environment-friendly enzyme disinfectant is prepared from the following components in percentage by weight: 20-35% of citric acid, 10-20% of malic acid, 10-20% of tartaric acid and the balance antibacterial fermentation extract, wherein the antibacterial fermentation extract is prepared by the following steps: (1) taking eucalyptus leaves and stems, leaves and / or barks of jatropha curcas, adding water, and boiling for 3-20 minutes to obtain boiled liquid; (2) after the boiled liquid is cooled, adding a fermentation strain for anaerobic fermentation for 1-3 months to obtain a fermented liquid; and (3) filtering the fermented liquid to obtain the antibacterial fermentation extract. The disinfectant has the advantages of broad-spectrum disinfection effect, no toxic or side effect and stable quality.

The invention provides a jatropha curcas ribosome inactivation protein JcRIP12 coding gene, a codon-optimized jatropha curcas ribosome inactivation protein JcRIP12 coding gene, and a jatropha curcas ribosome inactivation protein JcRIP12 coded by the two coding genes. The jatropha curcas ribosome-inactivating protein JcRIP12 is used for preparing the jatropha curcas ribosome-inactivating protein JcRIP12, and an in-vitro anti-tumor experiment proves that the jatropha curcas ribosome-inactivating protein JcRIP12 has good anti-tumor activity on human non-small cell lung cancer cells, human osteosarcoma cells, human liver cancer cells or human gastric cancer cells. Therefore, the invention also provides application of the jatropha curcas ribosome-inactivating protein JcRIP12 in preparation of medicines for treating tumors. According to the invention, the variety of jatropha curcas ribosome inactivating proteins with anti-tumor activity is enriched, and support can be provided for research and development of anti-tumor drugs; and the problems of tedious separation and purification process, complex process, low production efficiency and relatively high production cost of separation and purification of jatropha curcas ribosome inactivated protein from jatropha curcas in the prior art are solved.