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Method for direct regeneration of adventitious bud from Jatropha curcas petiole explant

A technology of explants and tree petioles, applied in the field of plant biology, can solve the problems of low regeneration rate, poor quality of regenerated buds, long regeneration cycle, etc., achieve the effect of simple method, reduce negative impact, and shorten the cultivation cycle

Inactive Publication Date: 2013-09-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a method for directly regenerating adventitious buds from Jatropha curcas petiole explants in order to overcome the defects of low regeneration rate, poor quality of regenerated buds and long regeneration cycle in the prior art when explants of Jatropha curcas petiole regenerate adventitious buds

Method used

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  • Method for direct regeneration of adventitious bud from Jatropha curcas petiole explant
  • Method for direct regeneration of adventitious bud from Jatropha curcas petiole explant
  • Method for direct regeneration of adventitious bud from Jatropha curcas petiole explant

Examples

Experimental program
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Effect test

Embodiment 1

[0030] S1. Preparation of Vessels for Short-Term Cytokinin Solution Treatment of Explants

[0031] Select a petri dish with a diameter of 9 cm, place it in a high-pressure steam sterilizer, and sterilize it at 121 °C and 0.1 MPa for 20 minutes.

[0032] S2. Preparation of phenylthiadiazolyl urea (TDZ) treatment solution.

[0033] Accurately weigh a certain amount of TDZ powder, fully dissolve it with 1 N NaOH solution, and then dilute it with deionized water to prepare 0, 0.5, 1, 2, 3, 6, 12 mg / L TDZ treatment solutions. Use 1 N HCl solution to adjust the pH of the TDZ treatment solution to 5.8-6.0; filter and sterilize the prepared TDZ treatment solution with a 0.22 micron water filter before use.

[0034] S3. Surface sterilization of Jatropha curcas petiole and acquisition of petiole explant

[0035] Use scissors to cut a number of petioles of similar length near the top of the stem from an adult Jatropha curcas tree. After rinsing the petioles with tap water for 3...

Embodiment 2

[0051] S1. Preparation of the container for short-term cytokinin solution treatment of explants: same as in Example 1.

[0052] S2. Prepare a 3 mg / L phenylthiadiazolyl urea (TDZ) solution, and the specific preparation method is the same as that in Example 1.

[0053] S3. Surface sterilization of Jatropha curcas petioles and acquisition of petiole explants: the specific method is the same as in Example 1.

[0054] S4. Cytokinin (TDZ) solution treatment of Jatropha curcas petiole explants

[0055] In the ultra-clean workbench, place the cut petiole explants in the sterilized petri dishes prepared in the above step S1, and pour the 3 mg / L of the above-mentioned step S2 into each of the petri dishes. TDZ solution until the petiole explants were submerged, covered with the lid of the petri dish, and left to stand for 0, 5, 10, 20, 40, and 80 minutes respectively (the soaking treatment time of the control group was 0 minutes means that the petiole explants were not ...

Embodiment 3

[0064] S1. Preparation of the container for short-term cytokinin solution treatment of explants: same as in Example 1.

[0065] S2. Prepare a 3 mg / L phenylthiadiazolyl urea (TDZ) solution, and the specific preparation method is the same as in Example 1.

[0066] S3. Surface sterilization of Jatropha curcas petioles and acquisition of petiole explants: the specific method is the same as in Example 1.

[0067] S4. Cytokinin (TDZ) solution treatment of Jatropha curcas petiole explants

[0068] In the ultra-clean workbench, place the cut petiole explants in the sterilized petri dishes prepared in the above step S1, and pour the 3 mg / L TDZ prepared in the above step S2 into each of the petri dishes. Solution until the petiole explants are immersed in the petiole explants, cover the petri dish, and after standing for 5 minutes, pour off the TDZ solution, keep the petiole, take out all the petiole explants from the petri dish with sterilized tweezers, and place them ...

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Abstract

The invention specifically discloses a method for direct regeneration of an adventitious bud from a Jatropha curcas petiole explant, which belongs to the field of plant biotechnology. The method improves a traditional method for regeneration of the adventitious bud from the Jatropha curcas petiole explant; i.e., instead of addition of hormone into a culture medium which induces regeneration of the adventitious bud, a high-concentration thidiazuron solution is used for short-period soaking treatment of the Jatropha curcas petiole explant so as to give short-period but high-intensity hormone stimulation to explant cells, thereby allowing a part of the cells to undergo more highly efficiently direct redifferentiation so as to form more adventitious buds. With the method provided by the invention, regeneration efficiency and regeneration quality of the adventitious bud from the Jatropha curcas petiole explant are substantially improved. Moreover, the explant used in the method does not need to undergo routine stages of callus formation and adventitious bud propagation, so a regeneration culture period is substantially shortened, and the efficiency of operations related to biotechnological breeding of Jatropha curcas is greatly improved accordingly.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for directly regenerating adventitious buds from Jatropha curcas petiole explants. Background technique [0002] With the growing global demand for fossil fuels and the depletion of stored fossil fuels, there is growing interest in renewable biodiesel. Among the candidate plants for biodiesel production, Jatropha curcas ( Jatroha curcas L.) has obvious advantages. Its seeds have high oil content, and the oil content of its kernels can reach 40%~60%. Pesticide and pharmaceutical value. [0003] However, vigorously promoting the planting of Jatropha curcas is facing a series of problems. Although the oil content in Jatropha curcas seeds is high, the seed yield is not high; there are many active ingredients in the seed oil, but the composition of the oil still needs to be changed to directly replace fossil fuels ; Jatropha curcas has high environmental requirements, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘颖杨跃生刘振兰庄楚雄冯琪郭丹丹杨柳贤彭昌操陈晓阳
Owner SOUTH CHINA AGRI UNIV
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